Microlunatus sp. KUDC0627

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Microlunatus; unclassified Microlunatus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4484 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516Q565|A0A516Q565_9ACTN Carbohydrate ABC transporter permease OS=Microlunatus sp. KUDC0627 OX=2596828 GN=FOE78_05040 PE=3 SV=1
MM1 pKa = 7.83AIARR5 pKa = 11.84YY6 pKa = 6.2PTVVLDD12 pKa = 4.54CPDD15 pKa = 3.99PAALADD21 pKa = 5.39FYY23 pKa = 11.71AAMLDD28 pKa = 3.71WKK30 pKa = 10.89VEE32 pKa = 4.17PGDD35 pKa = 3.5DD36 pKa = 3.5WVDD39 pKa = 3.1IRR41 pKa = 11.84ADD43 pKa = 3.58YY44 pKa = 10.35GQGISFQQVKK54 pKa = 10.5DD55 pKa = 3.74YY56 pKa = 10.33TPPDD60 pKa = 3.3WPGQQQPQQFHH71 pKa = 6.96LDD73 pKa = 3.7VVVDD77 pKa = 4.45DD78 pKa = 5.73LDD80 pKa = 3.84TAEE83 pKa = 4.14QAVLEE88 pKa = 4.46LGATKK93 pKa = 10.02HH94 pKa = 5.74DD95 pKa = 4.13HH96 pKa = 6.2QPGTTFRR103 pKa = 11.84VFLDD107 pKa = 3.71PAGHH111 pKa = 7.03PFCLCQSS118 pKa = 3.57

Molecular weight: 13.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516PUA8|A0A516PUA8_9ACTN DUF3592 domain-containing protein OS=Microlunatus sp. KUDC0627 OX=2596828 GN=FOE78_01615 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84THH17 pKa = 6.24GFRR20 pKa = 11.84HH21 pKa = 6.29RR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.61GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4484

0

4484

1478000

28

3173

329.6

35.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.097 ± 0.045

0.733 ± 0.01

6.519 ± 0.033

5.16 ± 0.034

2.941 ± 0.02

8.861 ± 0.031

2.158 ± 0.017

4.811 ± 0.023

2.263 ± 0.03

10.149 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.883 ± 0.012

2.197 ± 0.02

5.611 ± 0.026

3.468 ± 0.02

7.653 ± 0.038

5.684 ± 0.027

5.898 ± 0.035

8.115 ± 0.034

1.602 ± 0.016

2.198 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski