Mycobacterium phage DRBy19

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3T787|A0A6M3T787_9CAUD Uncharacterized protein OS=Mycobacterium phage DRBy19 OX=2725612 GN=93 PE=4 SV=1
MM1 pKa = 7.52NNPEE5 pKa = 3.95LRR7 pKa = 11.84AVLTEE12 pKa = 4.1ALAGHH17 pKa = 5.59QPEE20 pKa = 4.6NYY22 pKa = 9.94GFNCSGCNWEE32 pKa = 4.16PANPGVTDD40 pKa = 3.57AAEE43 pKa = 4.27FAAHH47 pKa = 6.31QLDD50 pKa = 3.9VLDD53 pKa = 4.69AVPGVAVIQLPEE65 pKa = 4.56PDD67 pKa = 4.52ADD69 pKa = 4.06HH70 pKa = 7.35GDD72 pKa = 4.64GIAFWTAQEE81 pKa = 4.11VGVADD86 pKa = 3.82GMVVARR92 pKa = 11.84FDD94 pKa = 3.88PGNEE98 pKa = 3.83AIYY101 pKa = 10.33YY102 pKa = 7.43EE103 pKa = 4.59PSEE106 pKa = 4.0AWEE109 pKa = 4.29TVAALAAAAVVATGEE124 pKa = 4.23EE125 pKa = 4.33EE126 pKa = 3.93

Molecular weight: 13.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3T6P6|A0A6M3T6P6_9CAUD Helix-turn-helix DNA binding domain protein OS=Mycobacterium phage DRBy19 OX=2725612 GN=61 PE=4 SV=1
MM1 pKa = 7.46ARR3 pKa = 11.84LGTTWQAWLGMARR16 pKa = 11.84LGRR19 pKa = 11.84AWLGAARR26 pKa = 11.84HH27 pKa = 5.19GRR29 pKa = 11.84RR30 pKa = 11.84GGAWRR35 pKa = 11.84GEE37 pKa = 4.02ARR39 pKa = 11.84PGTARR44 pKa = 11.84RR45 pKa = 11.84GMAGAARR52 pKa = 11.84QGG54 pKa = 3.23

Molecular weight: 5.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

18541

29

1182

178.3

19.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.485 ± 0.478

1.176 ± 0.147

6.812 ± 0.253

5.992 ± 0.295

2.983 ± 0.177

8.813 ± 0.565

2.157 ± 0.176

4.293 ± 0.176

3.468 ± 0.196

7.27 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.14

3.366 ± 0.152

5.847 ± 0.217

3.409 ± 0.181

7.006 ± 0.402

5.927 ± 0.317

6.672 ± 0.209

7.319 ± 0.279

2.244 ± 0.157

2.503 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski