Pelobacter acetylenicus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Syntrophotaleaceae; Syntrophotalea

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2796 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3GEX8|A0A1L3GEX8_PELAE RNA polymerase subunit sigma OS=Pelobacter acetylenicus OX=29542 GN=A7E75_05310 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 9.77KK3 pKa = 9.7WSVLLVSVFMLAVGFASQAQAKK25 pKa = 9.84IEE27 pKa = 4.1VEE29 pKa = 3.81GDD31 pKa = 3.39VYY33 pKa = 11.44AGIWDD38 pKa = 3.67KK39 pKa = 11.75YY40 pKa = 6.29MWRR43 pKa = 11.84GFNLSDD49 pKa = 4.46SRR51 pKa = 11.84PTIQAGIDD59 pKa = 3.48LTLGSGWTLSTWHH72 pKa = 6.08NWQLTSGPNWNAGEE86 pKa = 4.12LNEE89 pKa = 4.23TDD91 pKa = 4.56VILTYY96 pKa = 11.11AFDD99 pKa = 4.65LGDD102 pKa = 4.57MISMSVGDD110 pKa = 3.83IWYY113 pKa = 8.69MIEE116 pKa = 5.12GEE118 pKa = 4.28DD119 pKa = 3.73TNEE122 pKa = 3.84LFVTFTLNTLLSPNLKK138 pKa = 9.72ISYY141 pKa = 9.61DD142 pKa = 3.24WDD144 pKa = 3.58RR145 pKa = 11.84AEE147 pKa = 4.51EE148 pKa = 4.64DD149 pKa = 3.63GLFYY153 pKa = 11.08SFDD156 pKa = 3.37ISHH159 pKa = 6.54TFDD162 pKa = 3.39LGQWVPNTALNLGALVSYY180 pKa = 9.58NQHH183 pKa = 6.5ADD185 pKa = 3.37GTVADD190 pKa = 3.8YY191 pKa = 11.27AGWHH195 pKa = 5.95NYY197 pKa = 7.78EE198 pKa = 4.27LSASIDD204 pKa = 3.59YY205 pKa = 10.98ALNDD209 pKa = 3.5QMTISPIFIFSSPISSAAKK228 pKa = 10.25DD229 pKa = 4.08LIDD232 pKa = 4.13TEE234 pKa = 4.31TAAALNLTFAFF245 pKa = 4.57

Molecular weight: 27.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3GCS0|A0A1L3GCS0_PELAE Uncharacterized protein OS=Pelobacter acetylenicus OX=29542 GN=A7E75_00875 PE=4 SV=1
MM1 pKa = 6.89TAVPVAVPVAVPVAVPAVVRR21 pKa = 11.84AAVPVVVPVVVPVAVPAVVRR41 pKa = 11.84AAVPVVVPVAVPVAVPVAVPVAVPAVVPAVVPAVVRR77 pKa = 11.84AVVRR81 pKa = 11.84AVVPAVVPAVVPAVVPAAVPVAVPVAVPVAVPVAVPAVVPAVVPVVVPVVVPVAVPVAVPAVVRR145 pKa = 11.84AAVRR149 pKa = 11.84AAVRR153 pKa = 11.84AAVRR157 pKa = 11.84VAVPVAVPVAVPVV170 pKa = 3.41

Molecular weight: 16.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2796

0

2796

902541

38

2902

322.8

35.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.93 ± 0.059

1.447 ± 0.023

5.547 ± 0.041

6.258 ± 0.048

3.972 ± 0.031

8.144 ± 0.047

2.167 ± 0.019

5.543 ± 0.038

4.03 ± 0.046

11.066 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.486 ± 0.019

2.975 ± 0.025

4.66 ± 0.033

3.767 ± 0.032

7.199 ± 0.047

5.268 ± 0.035

4.735 ± 0.026

7.139 ± 0.039

1.097 ± 0.017

2.569 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski