Mycobacterium phage Demsculpinboyz

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Avanivirus; unclassified Avanivirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 117 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1G9Y5|A0A2D1G9Y5_9CAUD Uncharacterized protein OS=Mycobacterium phage Demsculpinboyz OX=2041528 GN=SEA_DEMSCULPINBOYZ_96 PE=4 SV=1
MM1 pKa = 7.71SDD3 pKa = 5.03CLLCDD8 pKa = 3.92HH9 pKa = 7.15PRR11 pKa = 11.84STHH14 pKa = 5.11TPQCRR19 pKa = 11.84TRR21 pKa = 11.84LGVDD25 pKa = 2.95ADD27 pKa = 3.66DD28 pKa = 3.59MTRR31 pKa = 11.84YY32 pKa = 6.71TQCLCPGFEE41 pKa = 4.22GTEE44 pKa = 4.11EE45 pKa = 5.26EE46 pKa = 4.28EE47 pKa = 4.68DD48 pKa = 3.38TWLDD52 pKa = 3.26

Molecular weight: 5.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GA55|A0A2D1GA55_9CAUD Uncharacterized protein OS=Mycobacterium phage Demsculpinboyz OX=2041528 GN=SEA_DEMSCULPINBOYZ_94 PE=4 SV=1
MM1 pKa = 7.33TGRR4 pKa = 11.84VLTPVEE10 pKa = 4.17VEE12 pKa = 3.91KK13 pKa = 11.13VAWLTRR19 pKa = 11.84MGWTATQIAEE29 pKa = 4.36QLGCHH34 pKa = 6.02HH35 pKa = 6.59RR36 pKa = 11.84TVQRR40 pKa = 11.84ARR42 pKa = 11.84AKK44 pKa = 10.14TGVAKK49 pKa = 9.89PKK51 pKa = 10.23PPALSTEE58 pKa = 4.0ILEE61 pKa = 4.32EE62 pKa = 4.03AARR65 pKa = 11.84MLADD69 pKa = 3.78GASQNEE75 pKa = 4.29VARR78 pKa = 11.84TLGVGQATISAHH90 pKa = 5.79FRR92 pKa = 11.84GQGWTRR98 pKa = 11.84EE99 pKa = 3.98QSIEE103 pKa = 3.92WISFIRR109 pKa = 11.84RR110 pKa = 11.84YY111 pKa = 10.31RR112 pKa = 11.84GVAA115 pKa = 2.99

Molecular weight: 12.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

117

0

117

18612

25

1181

159.1

17.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.337 ± 0.352

1.37 ± 0.173

6.372 ± 0.237

6.356 ± 0.282

2.864 ± 0.13

8.736 ± 0.554

2.262 ± 0.146

4.562 ± 0.161

3.707 ± 0.211

7.844 ± 0.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.181 ± 0.109

3.277 ± 0.146

5.738 ± 0.218

3.713 ± 0.195

6.678 ± 0.369

5.899 ± 0.326

6.265 ± 0.28

7.076 ± 0.25

2.359 ± 0.156

2.402 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski