Mycobacterium phage AltPhacts

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Pegunavirus; unclassified Pegunavirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1JW52|A0A2P1JW52_9CAUD Portal protein OS=Mycobacterium phage AltPhacts OX=2094128 GN=8 PE=4 SV=1
MM1 pKa = 8.05ADD3 pKa = 2.86QDD5 pKa = 4.35FYY7 pKa = 11.87FEE9 pKa = 4.3VDD11 pKa = 3.13GQRR14 pKa = 11.84FDD16 pKa = 3.68QRR18 pKa = 11.84VEE20 pKa = 4.12AMAHH24 pKa = 5.96CDD26 pKa = 3.1LYY28 pKa = 10.97GYY30 pKa = 10.06HH31 pKa = 6.61YY32 pKa = 11.03SAIQWVDD39 pKa = 3.74PNAPWAA45 pKa = 4.04

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1JW51|A0A2P1JW51_9CAUD Uncharacterized protein OS=Mycobacterium phage AltPhacts OX=2094128 GN=5 PE=4 SV=1
MM1 pKa = 7.57RR2 pKa = 11.84VAIPPIEE9 pKa = 4.15RR10 pKa = 11.84FYY12 pKa = 11.56GLTRR16 pKa = 11.84RR17 pKa = 11.84EE18 pKa = 4.3GACLVWTGHH27 pKa = 6.08CVGTKK32 pKa = 7.94SRR34 pKa = 11.84RR35 pKa = 11.84PRR37 pKa = 11.84FRR39 pKa = 11.84PTTSQHH45 pKa = 6.19DD46 pKa = 3.79PSVYY50 pKa = 7.91AHH52 pKa = 6.22RR53 pKa = 11.84WIWEE57 pKa = 4.05QVRR60 pKa = 11.84GPIPEE65 pKa = 4.61GYY67 pKa = 10.15EE68 pKa = 3.63IDD70 pKa = 3.66HH71 pKa = 6.85LCRR74 pKa = 11.84NGLCVEE80 pKa = 4.26ITHH83 pKa = 6.85LEE85 pKa = 3.94PVPPSINAEE94 pKa = 3.95RR95 pKa = 11.84TRR97 pKa = 11.84LGVCRR102 pKa = 11.84SGRR105 pKa = 11.84HH106 pKa = 6.39DD107 pKa = 3.53LTDD110 pKa = 3.65PRR112 pKa = 11.84NVQWDD117 pKa = 3.58EE118 pKa = 3.54KK119 pKa = 10.41GRR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84GCLSCRR129 pKa = 11.84RR130 pKa = 11.84DD131 pKa = 3.35RR132 pKa = 11.84EE133 pKa = 4.08RR134 pKa = 11.84KK135 pKa = 8.28RR136 pKa = 11.84QRR138 pKa = 11.84LRR140 pKa = 11.84RR141 pKa = 11.84GGQQ144 pKa = 2.85

Molecular weight: 16.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99

0

99

21424

32

1991

216.4

23.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.127 ± 0.355

1.125 ± 0.155

6.465 ± 0.23

5.937 ± 0.323

2.273 ± 0.104

9.713 ± 0.66

2.105 ± 0.192

4.626 ± 0.143

2.913 ± 0.149

7.944 ± 0.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.068 ± 0.092

2.917 ± 0.187

6.035 ± 0.238

3.067 ± 0.115

6.81 ± 0.321

4.91 ± 0.197

7.1 ± 0.2

7.543 ± 0.174

1.839 ± 0.147

2.483 ± 0.107
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski