Enterococcus phage phiFL4A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2J065|D2J065_9CAUD Uncharacterized protein gp43 OS=Enterococcus phage phiFL4A OX=673839 GN=gp43 PE=4 SV=1
MM1 pKa = 7.52YY2 pKa = 10.47SPQTFFLQEE11 pKa = 3.95VKK13 pKa = 10.55GQKK16 pKa = 9.6PDD18 pKa = 3.44GLGGLIDD25 pKa = 3.78DD26 pKa = 3.97WGLFKK31 pKa = 10.72EE32 pKa = 4.37VSGYY36 pKa = 10.16IDD38 pKa = 4.81LVTGTDD44 pKa = 3.13EE45 pKa = 4.37TTKK48 pKa = 10.68QNAFVEE54 pKa = 4.82EE55 pKa = 4.45STHH58 pKa = 7.71ILIIPEE64 pKa = 4.71FIEE67 pKa = 5.59GITDD71 pKa = 3.15NMRR74 pKa = 11.84VVDD77 pKa = 3.87QTNRR81 pKa = 11.84YY82 pKa = 8.96YY83 pKa = 10.93DD84 pKa = 3.03ITYY87 pKa = 10.07SDD89 pKa = 3.7NPVGIKK95 pKa = 9.35HH96 pKa = 6.41HH97 pKa = 6.1NEE99 pKa = 3.28IYY101 pKa = 10.77LKK103 pKa = 10.88FEE105 pKa = 4.2GVLSGEE111 pKa = 4.41EE112 pKa = 3.83

Molecular weight: 12.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2J050|D2J050_9CAUD DNA helicase OS=Enterococcus phage phiFL4A OX=673839 GN=gp28 PE=4 SV=1
MM1 pKa = 7.39QIEE4 pKa = 4.21NDD6 pKa = 3.13IEE8 pKa = 4.51KK9 pKa = 11.02YY10 pKa = 10.52LIRR13 pKa = 11.84QIKK16 pKa = 8.07RR17 pKa = 11.84TGALCYY23 pKa = 10.35KK24 pKa = 8.45FTSPGTRR31 pKa = 11.84GVPDD35 pKa = 5.07RR36 pKa = 11.84IILYY40 pKa = 9.11QGNVFFVEE48 pKa = 4.73LKK50 pKa = 10.71RR51 pKa = 11.84PGGKK55 pKa = 8.7PRR57 pKa = 11.84KK58 pKa = 8.8DD59 pKa = 3.14QLKK62 pKa = 9.96IIEE65 pKa = 4.48KK66 pKa = 10.23FKK68 pKa = 10.73EE69 pKa = 3.55QLIPVFVIDD78 pKa = 4.2SKK80 pKa = 11.42QGVDD84 pKa = 3.25TLIYY88 pKa = 10.87AMQSGIARR96 pKa = 11.84VMPDD100 pKa = 3.11MPRR103 pKa = 11.84KK104 pKa = 9.98KK105 pKa = 10.07EE106 pKa = 3.77

Molecular weight: 12.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11804

44

1077

214.6

24.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.091 ± 0.529

0.517 ± 0.094

6.108 ± 0.328

8.48 ± 0.611

4.236 ± 0.264

5.812 ± 0.362

1.339 ± 0.187

6.955 ± 0.369

8.938 ± 0.401

8.412 ± 0.435

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.177 ± 0.117

5.202 ± 0.333

3.101 ± 0.237

3.821 ± 0.184

3.77 ± 0.36

5.795 ± 0.27

6.455 ± 0.34

6.422 ± 0.387

1.389 ± 0.202

3.982 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski