Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849) (Mycobacterium hassiacum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycolicibacterium; Mycolicibacterium hassiacum

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4959 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K5BKG8|K5BKG8_MYCHD PucR C-terminal helix-turn-helix domain protein OS=Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849) OX=1122247 GN=pmfR_1 PE=3 SV=1
MM1 pKa = 8.11RR2 pKa = 11.84DD3 pKa = 3.51QLPPGLPPDD12 pKa = 4.82PFADD16 pKa = 4.67DD17 pKa = 3.72PWDD20 pKa = 3.88PSAALEE26 pKa = 4.63AIEE29 pKa = 5.42PGQQLDD35 pKa = 3.76PEE37 pKa = 4.07EE38 pKa = 4.28RR39 pKa = 11.84AAVEE43 pKa = 4.58ADD45 pKa = 3.48LADD48 pKa = 3.99LAVYY52 pKa = 8.4EE53 pKa = 4.77ALLAHH58 pKa = 6.71KK59 pKa = 9.46GIRR62 pKa = 11.84GLVVACDD69 pKa = 3.77EE70 pKa = 4.47CQQDD74 pKa = 4.77HH75 pKa = 5.22YY76 pKa = 11.02HH77 pKa = 6.67DD78 pKa = 4.0WDD80 pKa = 3.57MLRR83 pKa = 11.84ANLLQLLVDD92 pKa = 4.02GTVRR96 pKa = 11.84PHH98 pKa = 6.32EE99 pKa = 4.41PAYY102 pKa = 10.49DD103 pKa = 4.05PQPDD107 pKa = 4.14AYY109 pKa = 9.77VTWDD113 pKa = 3.35YY114 pKa = 11.62CRR116 pKa = 11.84GYY118 pKa = 11.36ADD120 pKa = 3.87AALNEE125 pKa = 4.31ATPDD129 pKa = 3.75PDD131 pKa = 3.58SFRR134 pKa = 5.07

Molecular weight: 14.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K5BIF4|K5BIF4_MYCHD Uncharacterized protein OS=Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849) OX=1122247 GN=C731_4738 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVANRR37 pKa = 11.84RR38 pKa = 11.84AKK40 pKa = 10.04GRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84LTAA47 pKa = 4.0

Molecular weight: 5.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4959

0

4959

1513784

32

3928

305.3

32.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.943 ± 0.047

0.837 ± 0.01

6.265 ± 0.028

5.683 ± 0.033

3.004 ± 0.019

8.753 ± 0.03

2.162 ± 0.016

4.293 ± 0.021

2.159 ± 0.024

9.794 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.925 ± 0.014

2.178 ± 0.018

6.186 ± 0.034

2.867 ± 0.019

7.905 ± 0.037

4.759 ± 0.017

5.916 ± 0.025

8.665 ± 0.03

1.496 ± 0.015

2.209 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski