Fly associated circular virus 4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus; Porprismacovirus flas1

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346BPB5|A0A346BPB5_9VIRU Putative capsid protein OS=Fly associated circular virus 4 OX=2293284 PE=4 SV=1
MM1 pKa = 7.21FVQVSEE7 pKa = 4.54TYY9 pKa = 10.7DD10 pKa = 3.44LSTQIGKK17 pKa = 8.67MGFVAIHH24 pKa = 6.09TPDD27 pKa = 3.21LQLIKK32 pKa = 10.9KK33 pKa = 6.71MWKK36 pKa = 9.37GLLMNHH42 pKa = 7.22RR43 pKa = 11.84YY44 pKa = 9.96YY45 pKa = 11.15KK46 pKa = 10.54FQSCDD51 pKa = 2.92LAMACASMLPADD63 pKa = 4.27PLQVGVEE70 pKa = 4.12SGEE73 pKa = 4.09IAPQDD78 pKa = 3.59MFNSILYY85 pKa = 9.77RR86 pKa = 11.84AVSNDD91 pKa = 3.03SYY93 pKa = 11.33NTILNRR99 pKa = 11.84IQNKK103 pKa = 7.58YY104 pKa = 8.34QSPVSGVDD112 pKa = 3.29KK113 pKa = 11.23NSVISDD119 pKa = 3.78NSDD122 pKa = 2.96SLADD126 pKa = 3.45VDD128 pKa = 5.81QFDD131 pKa = 4.89LYY133 pKa = 11.28YY134 pKa = 11.12GLLAEE139 pKa = 4.27PSGWRR144 pKa = 11.84KK145 pKa = 10.0AMPQNGLQMSNLYY158 pKa = 10.37PMVFQLVSNVGNNTGANTGSAFSLSKK184 pKa = 10.37LNSMFSSPGLGDD196 pKa = 3.95DD197 pKa = 3.44QASAGGVVQYY207 pKa = 11.87AMFRR211 pKa = 11.84GPSMRR216 pKa = 11.84MPRR219 pKa = 11.84IPTFGISSTSSEE231 pKa = 4.22IIGDD235 pKa = 3.77DD236 pKa = 3.23VTAIGEE242 pKa = 4.31FQSTPITYY250 pKa = 8.6VACIVLPPAKK260 pKa = 10.49LNVLYY265 pKa = 10.86YY266 pKa = 9.86RR267 pKa = 11.84LKK269 pKa = 9.03VTWTIEE275 pKa = 3.79FSEE278 pKa = 5.2PYY280 pKa = 9.48PATEE284 pKa = 3.57IANYY288 pKa = 9.64TSIANIGNQSYY299 pKa = 10.88GSDD302 pKa = 3.41YY303 pKa = 11.05DD304 pKa = 3.66AQSNFMSVKK313 pKa = 9.53TSSVDD318 pKa = 3.26AKK320 pKa = 10.62DD321 pKa = 4.53ANVQKK326 pKa = 10.69IMEE329 pKa = 4.48SGRR332 pKa = 3.55

Molecular weight: 36.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346BPB5|A0A346BPB5_9VIRU Putative capsid protein OS=Fly associated circular virus 4 OX=2293284 PE=4 SV=1
MM1 pKa = 7.06VKK3 pKa = 10.33VYY5 pKa = 10.77LLTVPLVHH13 pKa = 7.45RR14 pKa = 11.84IQFDD18 pKa = 3.57SPLYY22 pKa = 8.97RR23 pKa = 11.84WRR25 pKa = 11.84HH26 pKa = 4.54SSSYY30 pKa = 7.17STKK33 pKa = 9.21RR34 pKa = 11.84AIRR37 pKa = 11.84ILIEE41 pKa = 4.16SLDD44 pKa = 3.78CKK46 pKa = 10.21RR47 pKa = 11.84WIVARR52 pKa = 11.84EE53 pKa = 3.87RR54 pKa = 11.84GRR56 pKa = 11.84GGYY59 pKa = 8.2EE60 pKa = 3.53HH61 pKa = 6.8LQVRR65 pKa = 11.84VEE67 pKa = 4.19TSADD71 pKa = 3.26NFFDD75 pKa = 3.43RR76 pKa = 11.84VSRR79 pKa = 11.84VLPCAHH85 pKa = 6.74IEE87 pKa = 4.17EE88 pKa = 4.78STSAYY93 pKa = 10.08SDD95 pKa = 2.73WYY97 pKa = 8.95EE98 pKa = 4.14RR99 pKa = 11.84KK100 pKa = 8.93EE101 pKa = 3.93GRR103 pKa = 11.84YY104 pKa = 7.19VTSSDD109 pKa = 3.83LLPGRR114 pKa = 11.84TKK116 pKa = 10.69VLKK119 pKa = 10.32QRR121 pKa = 11.84FGCFYY126 pKa = 9.86PFQKK130 pKa = 10.23RR131 pKa = 11.84VLRR134 pKa = 11.84ALQSTNDD141 pKa = 3.49RR142 pKa = 11.84EE143 pKa = 4.44VVVWYY148 pKa = 9.56DD149 pKa = 3.2QTGNAGKK156 pKa = 9.92SWLKK160 pKa = 9.6GALWEE165 pKa = 4.73RR166 pKa = 11.84GLAYY170 pKa = 9.62IIQPMSSISVMVQDD184 pKa = 4.78VADD187 pKa = 4.02EE188 pKa = 4.25YY189 pKa = 11.32LRR191 pKa = 11.84HH192 pKa = 6.82GYY194 pKa = 9.01RR195 pKa = 11.84QYY197 pKa = 11.69LVIDD201 pKa = 4.22IPRR204 pKa = 11.84TWKK207 pKa = 9.79WSEE210 pKa = 4.01DD211 pKa = 3.82LYY213 pKa = 11.44CAIEE217 pKa = 4.27TIKK220 pKa = 11.03DD221 pKa = 3.63GLLKK225 pKa = 10.54DD226 pKa = 3.47PRR228 pKa = 11.84YY229 pKa = 10.44NSEE232 pKa = 4.03TVNIAGVQVLILCNTLPKK250 pKa = 9.99LDD252 pKa = 4.24KK253 pKa = 10.94LSVDD257 pKa = 2.76RR258 pKa = 11.84WIILNRR264 pKa = 11.84DD265 pKa = 3.19GTRR268 pKa = 11.84RR269 pKa = 11.84GSFSS273 pKa = 3.05

Molecular weight: 31.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

605

273

332

302.5

34.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.95 ± 1.015

1.322 ± 0.332

6.116 ± 0.073

3.967 ± 0.758

3.306 ± 0.484

6.281 ± 0.513

1.157 ± 0.44

6.116 ± 0.073

4.628 ± 0.326

8.43 ± 0.953

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.14 ± 1.332

4.628 ± 1.347

4.298 ± 0.653

4.628 ± 0.63

6.116 ± 2.702

10.248 ± 1.19

4.793 ± 0.021

7.603 ± 0.536

1.983 ± 0.857

5.289 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski