Amino acid dipepetide frequency for Sparus aurata (Gilthead sea bream)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.046AlaAla: 6.046 ± 0.026	1.272AlaCys: 1.272 ± 0.008	3.21AlaAsp: 3.21 ± 0.009	4.564AlaGlu: 4.564 ± 0.015	2.429AlaPhe: 2.429 ± 0.009	4.256AlaGly: 4.256 ± 0.014	1.451AlaHis: 1.451 ± 0.006	2.838AlaIle: 2.838 ± 0.009	3.351AlaLys: 3.351 ± 0.013	6.451AlaLeu: 6.451 ± 0.015	1.614AlaMet: 1.614 ± 0.006	2.227AlaAsn: 2.227 ± 0.007	3.328AlaPro: 3.328 ± 0.017	2.846AlaGln: 2.846 ± 0.011	3.091AlaArg: 3.091 ± 0.01	5.188AlaSer: 5.188 ± 0.013	3.453AlaThr: 3.453 ± 0.011	4.95AlaVal: 4.95 ± 0.015	0.654AlaTrp: 0.654 ± 0.004	1.587AlaTyr: 1.587 ± 0.006	0.0AlaXaa: 0.0 ± 0.0
Cys	1.19CysAla: 1.19 ± 0.007	0.643CysCys: 0.643 ± 0.005	1.185CysAsp: 1.185 ± 0.009	1.296CysGlu: 1.296 ± 0.008	0.95CysPhe: 0.95 ± 0.005	1.549CysGly: 1.549 ± 0.01	0.667CysHis: 0.667 ± 0.005	1.059CysIle: 1.059 ± 0.006	1.168CysLys: 1.168 ± 0.006	2.266CysLeu: 2.266 ± 0.01	0.486CysMet: 0.486 ± 0.003	0.847CysAsn: 0.847 ± 0.006	1.261CysPro: 1.261 ± 0.008	1.07CysGln: 1.07 ± 0.007	1.319CysArg: 1.319 ± 0.007	2.139CysSer: 2.139 ± 0.014	1.21CysThr: 1.21 ± 0.007	1.724CysVal: 1.724 ± 0.01	0.307CysTrp: 0.307 ± 0.003	0.648CysTyr: 0.648 ± 0.004	0.0CysXaa: 0.0 ± 0.0
Asp	2.958AspAla: 2.958 ± 0.009	1.19AspCys: 1.19 ± 0.008	3.195AspAsp: 3.195 ± 0.013	3.777AspGlu: 3.777 ± 0.011	2.21AspPhe: 2.21 ± 0.007	3.726AspGly: 3.726 ± 0.013	1.237AspHis: 1.237 ± 0.006	2.853AspIle: 2.853 ± 0.01	2.821AspLys: 2.821 ± 0.009	5.022AspLeu: 5.022 ± 0.012	1.325AspMet: 1.325 ± 0.005	2.045AspAsn: 2.045 ± 0.009	2.868AspPro: 2.868 ± 0.01	1.995AspGln: 1.995 ± 0.007	2.794AspArg: 2.794 ± 0.011	4.354AspSer: 4.354 ± 0.011	2.687AspThr: 2.687 ± 0.009	3.409AspVal: 3.409 ± 0.012	0.716AspTrp: 0.716 ± 0.005	1.629AspTyr: 1.629 ± 0.005	0.0AspXaa: 0.0 ± 0.0
Glu	4.687GluAla: 4.687 ± 0.015	1.303GluCys: 1.303 ± 0.009	4.334GluAsp: 4.334 ± 0.012	7.441GluGlu: 7.441 ± 0.034	2.021GluPhe: 2.021 ± 0.007	4.136GluGly: 4.136 ± 0.012	1.457GluHis: 1.457 ± 0.006	2.964GluIle: 2.964 ± 0.009	4.567GluLys: 4.567 ± 0.02	6.308GluLeu: 6.308 ± 0.017	1.758GluMet: 1.758 ± 0.007	2.697GluAsn: 2.697 ± 0.009	2.701GluPro: 2.701 ± 0.01	3.04GluGln: 3.04 ± 0.012	4.213GluArg: 4.213 ± 0.014	4.166GluSer: 4.166 ± 0.012	3.41GluThr: 3.41 ± 0.011	4.448GluVal: 4.448 ± 0.013	0.735GluTrp: 0.735 ± 0.004	1.697GluTyr: 1.697 ± 0.006	0.0GluXaa: 0.0 ± 0.0
Phe	1.992PheAla: 1.992 ± 0.007	0.985PheCys: 0.985 ± 0.005	1.89PheAsp: 1.89 ± 0.007	1.969PheGlu: 1.969 ± 0.007	1.743PhePhe: 1.743 ± 0.008	2.287PheGly: 2.287 ± 0.009	1.041PheHis: 1.041 ± 0.005	2.079PheIle: 2.079 ± 0.009	1.91PheLys: 1.91 ± 0.008	4.015PheLeu: 4.015 ± 0.013	0.874PheMet: 0.874 ± 0.005	1.58PheAsn: 1.58 ± 0.006	1.809PhePro: 1.809 ± 0.006	1.64PheGln: 1.64 ± 0.006	1.915PheArg: 1.915 ± 0.007	3.417PheSer: 3.417 ± 0.01	2.47PheThr: 2.47 ± 0.009	2.357PheVal: 2.357 ± 0.008	0.498PheTrp: 0.498 ± 0.004	1.293PheTyr: 1.293 ± 0.005	0.0PheXaa: 0.0 ± 0.0
Gly	3.937GlyAla: 3.937 ± 0.015	1.248GlyCys: 1.248 ± 0.006	3.256GlyAsp: 3.256 ± 0.011	4.09GlyGlu: 4.09 ± 0.013	2.462GlyPhe: 2.462 ± 0.009	5.243GlyGly: 5.243 ± 0.022	1.653GlyHis: 1.653 ± 0.007	2.686GlyIle: 2.686 ± 0.01	3.603GlyLys: 3.603 ± 0.011	5.469GlyLeu: 5.469 ± 0.013	1.519GlyMet: 1.519 ± 0.006	2.467GlyAsn: 2.467 ± 0.009	3.139GlyPro: 3.139 ± 0.02	2.722GlyGln: 2.722 ± 0.011	3.62GlyArg: 3.62 ± 0.011	5.451GlySer: 5.451 ± 0.018	3.437GlyThr: 3.437 ± 0.011	4.083GlyVal: 4.083 ± 0.014	0.788GlyTrp: 0.788 ± 0.006	1.919GlyTyr: 1.919 ± 0.008	0.0GlyXaa: 0.0 ± 0.0
His	1.308HisAla: 1.308 ± 0.006	0.789HisCys: 0.789 ± 0.006	1.024HisAsp: 1.024 ± 0.006	1.224HisGlu: 1.224 ± 0.005	1.089HisPhe: 1.089 ± 0.005	1.531HisGly: 1.531 ± 0.007	1.019HisHis: 1.019 ± 0.008	1.388HisIle: 1.388 ± 0.007	1.28HisLys: 1.28 ± 0.006	2.704HisLeu: 2.704 ± 0.009	0.683HisMet: 0.683 ± 0.005	1.05HisAsn: 1.05 ± 0.005	1.546HisPro: 1.546 ± 0.008	1.233HisGln: 1.233 ± 0.007	1.608HisArg: 1.608 ± 0.008	2.353HisSer: 2.353 ± 0.008	1.739HisThr: 1.739 ± 0.012	1.501HisVal: 1.501 ± 0.006	0.35HisTrp: 0.35 ± 0.003	0.871HisTyr: 0.871 ± 0.005	0.0HisXaa: 0.0 ± 0.0
Ile	2.623IleAla: 2.623 ± 0.009	1.104IleCys: 1.104 ± 0.006	2.264IleAsp: 2.264 ± 0.007	2.487IleGlu: 2.487 ± 0.008	1.971IlePhe: 1.971 ± 0.009	2.42IleGly: 2.42 ± 0.009	1.354IleHis: 1.354 ± 0.006	2.58IleIle: 2.58 ± 0.009	2.676IleLys: 2.676 ± 0.009	4.463IleLeu: 4.463 ± 0.014	1.164IleMet: 1.164 ± 0.005	2.107IleAsn: 2.107 ± 0.008	2.456IlePro: 2.456 ± 0.009	2.262IleGln: 2.262 ± 0.008	2.55IleArg: 2.55 ± 0.007	3.838IleSer: 3.838 ± 0.011	2.937IleThr: 2.937 ± 0.01	2.715IleVal: 2.715 ± 0.009	0.484IleTrp: 0.484 ± 0.004	1.498IleTyr: 1.498 ± 0.008	0.0IleXaa: 0.0 ± 0.0
Lys	3.726LysAla: 3.726 ± 0.012	1.038LysCys: 1.038 ± 0.006	3.261LysAsp: 3.261 ± 0.011	4.65LysGlu: 4.65 ± 0.021	1.715LysPhe: 1.715 ± 0.007	3.17LysGly: 3.17 ± 0.011	1.41LysHis: 1.41 ± 0.006	2.57LysIle: 2.57 ± 0.009	4.323LysLys: 4.323 ± 0.02	5.126LysLeu: 5.126 ± 0.014	1.466LysMet: 1.466 ± 0.007	2.291LysAsn: 2.291 ± 0.007	2.77LysPro: 2.77 ± 0.012	2.532LysGln: 2.532 ± 0.011	3.273LysArg: 3.273 ± 0.011	3.716LysSer: 3.716 ± 0.014	3.231LysThr: 3.231 ± 0.011	3.637LysVal: 3.637 ± 0.011	0.583LysTrp: 0.583 ± 0.004	1.562LysTyr: 1.562 ± 0.006	0.0LysXaa: 0.0 ± 0.0
Leu	6.056LeuAla: 6.056 ± 0.015	2.298LeuCys: 2.298 ± 0.009	5.032LeuAsp: 5.032 ± 0.014	6.427LeuGlu: 6.427 ± 0.019	3.64LeuPhe: 3.64 ± 0.014	5.117LeuGly: 5.117 ± 0.013	2.748LeuHis: 2.748 ± 0.009	4.05LeuIle: 4.05 ± 0.012	5.689LeuLys: 5.689 ± 0.015	10.234LeuLeu: 10.234 ± 0.024	2.203LeuMet: 2.203 ± 0.008	3.724LeuAsn: 3.724 ± 0.011	5.107LeuPro: 5.107 ± 0.013	5.429LeuGln: 5.429 ± 0.016	5.667LeuArg: 5.667 ± 0.015	8.322LeuSer: 8.322 ± 0.021	5.419LeuThr: 5.419 ± 0.014	5.543LeuVal: 5.543 ± 0.013	1.074LeuTrp: 1.074 ± 0.006	2.75LeuTyr: 2.75 ± 0.011	0.0LeuXaa: 0.0 ± 0.0
Met	1.876MetAla: 1.876 ± 0.008	0.526MetCys: 0.526 ± 0.004	1.449MetAsp: 1.449 ± 0.006	1.991MetGlu: 1.991 ± 0.008	0.935MetPhe: 0.935 ± 0.005	1.442MetGly: 1.442 ± 0.007	0.495MetHis: 0.495 ± 0.003	0.952MetIle: 0.952 ± 0.005	1.512MetLys: 1.512 ± 0.007	2.163MetLeu: 2.163 ± 0.008	0.714MetMet: 0.714 ± 0.005	0.963MetAsn: 0.963 ± 0.005	1.063MetPro: 1.063 ± 0.006	0.987MetGln: 0.987 ± 0.005	1.222MetArg: 1.222 ± 0.006	1.858MetSer: 1.858 ± 0.006	1.316MetThr: 1.316 ± 0.005	1.621MetVal: 1.621 ± 0.006	0.275MetTrp: 0.275 ± 0.003	0.69MetTyr: 0.69 ± 0.004	0.0MetXaa: 0.0 ± 0.0
Asn	2.143AsnAla: 2.143 ± 0.008	0.925AsnCys: 0.925 ± 0.006	1.773AsnAsp: 1.773 ± 0.009	2.098AsnGlu: 2.098 ± 0.007	1.48AsnPhe: 1.48 ± 0.006	2.756AsnGly: 2.756 ± 0.011	1.016AsnHis: 1.016 ± 0.005	2.273AsnIle: 2.273 ± 0.007	2.255AsnLys: 2.255 ± 0.008	3.736AsnLeu: 3.736 ± 0.009	1.081AsnMet: 1.081 ± 0.005	1.897AsnAsn: 1.897 ± 0.009	2.263AsnPro: 2.263 ± 0.008	1.761AsnGln: 1.761 ± 0.007	2.007AsnArg: 2.007 ± 0.007	3.149AsnSer: 3.149 ± 0.01	2.34AsnThr: 2.34 ± 0.008	2.37AsnVal: 2.37 ± 0.008	0.464AsnTrp: 0.464 ± 0.004	1.203AsnTyr: 1.203 ± 0.005	0.0AsnXaa: 0.0 ± 0.0
Pro	3.938ProAla: 3.938 ± 0.015	1.042ProCys: 1.042 ± 0.008	2.881ProAsp: 2.881 ± 0.009	3.698ProGlu: 3.698 ± 0.01	1.777ProPhe: 1.777 ± 0.008	3.852ProGly: 3.852 ± 0.022	1.451ProHis: 1.451 ± 0.008	1.911ProIle: 1.911 ± 0.007	2.417ProLys: 2.417 ± 0.011	4.688ProLeu: 4.688 ± 0.015	1.016ProMet: 1.016 ± 0.006	1.915ProAsn: 1.915 ± 0.008	4.994ProPro: 4.994 ± 0.03	2.537ProGln: 2.537 ± 0.012	2.583ProArg: 2.583 ± 0.009	5.178ProSer: 5.178 ± 0.018	3.028ProThr: 3.028 ± 0.018	3.681ProVal: 3.681 ± 0.01	0.54ProTrp: 0.54 ± 0.004	1.412ProTyr: 1.412 ± 0.007	0.0ProXaa: 0.0 ± 0.0
Gln	3.15GlnAla: 3.15 ± 0.012	1.014GlnCys: 1.014 ± 0.008	2.316GlnAsp: 2.316 ± 0.009	3.411GlnGlu: 3.411 ± 0.013	1.436GlnPhe: 1.436 ± 0.007	2.693GlnGly: 2.693 ± 0.011	1.346GlnHis: 1.346 ± 0.007	1.988GlnIle: 1.988 ± 0.006	2.547GlnLys: 2.547 ± 0.01	4.514GlnLeu: 4.514 ± 0.014	1.142GlnMet: 1.142 ± 0.005	1.713GlnAsn: 1.713 ± 0.006	2.445GlnPro: 2.445 ± 0.013	3.199GlnGln: 3.199 ± 0.022	3.032GlnArg: 3.032 ± 0.011	3.349GlnSer: 3.349 ± 0.011	2.542GlnThr: 2.542 ± 0.01	2.867GlnVal: 2.867 ± 0.01	0.575GlnTrp: 0.575 ± 0.004	1.306GlnTyr: 1.306 ± 0.006	0.0GlnXaa: 0.0 ± 0.0
Arg	3.366ArgAla: 3.366 ± 0.008	1.289ArgCys: 1.289 ± 0.011	2.968ArgAsp: 2.968 ± 0.01	3.891ArgGlu: 3.891 ± 0.014	2.012ArgPhe: 2.012 ± 0.006	3.428ArgGly: 3.428 ± 0.013	1.519ArgHis: 1.519 ± 0.006	2.492ArgIle: 2.492 ± 0.008	3.421ArgLys: 3.421 ± 0.01	5.384ArgLeu: 5.384 ± 0.014	1.312ArgMet: 1.312 ± 0.006	2.128ArgAsn: 2.128 ± 0.007	2.831ArgPro: 2.831 ± 0.011	2.693ArgGln: 2.693 ± 0.011	4.167ArgArg: 4.167 ± 0.016	4.301ArgSer: 4.301 ± 0.013	2.888ArgThr: 2.888 ± 0.01	3.383ArgVal: 3.383 ± 0.01	0.688ArgTrp: 0.688 ± 0.004	1.614ArgTyr: 1.614 ± 0.006	0.0ArgXaa: 0.0 ± 0.0
Ser	5.389SerAla: 5.389 ± 0.015	2.004SerCys: 2.004 ± 0.009	4.297SerAsp: 4.297 ± 0.012	4.71SerGlu: 4.71 ± 0.014	3.099SerPhe: 3.099 ± 0.009	5.452SerGly: 5.452 ± 0.015	2.194SerHis: 2.194 ± 0.01	3.402SerIle: 3.402 ± 0.01	3.859SerLys: 3.859 ± 0.012	8.19SerLeu: 8.19 ± 0.018	1.788SerMet: 1.788 ± 0.006	2.968SerAsn: 2.968 ± 0.011	5.537SerPro: 5.537 ± 0.023	3.697SerGln: 3.697 ± 0.012	4.314SerArg: 4.314 ± 0.013	9.432SerSer: 9.432 ± 0.032	4.619SerThr: 4.619 ± 0.015	5.459SerVal: 5.459 ± 0.014	0.98SerTrp: 0.98 ± 0.005	2.182SerTyr: 2.182 ± 0.007	0.0SerXaa: 0.0 ± 0.0
Thr	4.043ThrAla: 4.043 ± 0.019	1.442ThrCys: 1.442 ± 0.01	3.021ThrAsp: 3.021 ± 0.009	3.79ThrGlu: 3.79 ± 0.012	2.229ThrPhe: 2.229 ± 0.008	3.797ThrGly: 3.797 ± 0.012	1.519ThrHis: 1.519 ± 0.008	2.533ThrIle: 2.533 ± 0.009	2.693ThrLys: 2.693 ± 0.009	5.514ThrLeu: 5.514 ± 0.012	1.244ThrMet: 1.244 ± 0.006	1.989ThrAsn: 1.989 ± 0.009	3.5ThrPro: 3.5 ± 0.014	2.338ThrGln: 2.338 ± 0.008	2.491ThrArg: 2.491 ± 0.009	4.631ThrSer: 4.631 ± 0.014	3.562ThrThr: 3.562 ± 0.06	4.31ThrVal: 4.31 ± 0.014	0.683ThrTrp: 0.683 ± 0.005	1.523ThrTyr: 1.523 ± 0.007	0.0ThrXaa: 0.0 ± 0.0
Val	4.196ValAla: 4.196 ± 0.012	1.864ValCys: 1.864 ± 0.01	3.313ValAsp: 3.313 ± 0.01	4.127ValGlu: 4.127 ± 0.012	2.827ValPhe: 2.827 ± 0.011	3.561ValGly: 3.561 ± 0.012	1.622ValHis: 1.622 ± 0.006	3.14ValIle: 3.14 ± 0.009	3.69ValLys: 3.69 ± 0.01	6.353ValLeu: 6.353 ± 0.018	1.586ValMet: 1.586 ± 0.007	2.574ValAsn: 2.574 ± 0.008	3.17ValPro: 3.17 ± 0.01	2.805ValGln: 2.805 ± 0.008	3.42ValArg: 3.42 ± 0.01	5.364ValSer: 5.364 ± 0.013	4.091ValThr: 4.091 ± 0.016	4.526ValVal: 4.526 ± 0.016	0.813ValTrp: 0.813 ± 0.005	1.919ValTyr: 1.919 ± 0.008	0.0ValXaa: 0.0 ± 0.0
Trp	0.69TrpAla: 0.69 ± 0.004	0.262TrpCys: 0.262 ± 0.002	0.675TrpAsp: 0.675 ± 0.004	0.763TrpGlu: 0.763 ± 0.005	0.47TrpPhe: 0.47 ± 0.003	0.628TrpGly: 0.628 ± 0.005	0.278TrpHis: 0.278 ± 0.003	0.6TrpIle: 0.6 ± 0.004	0.72TrpLys: 0.72 ± 0.004	1.174TrpLeu: 1.174 ± 0.006	0.357TrpMet: 0.357 ± 0.003	0.534TrpAsn: 0.534 ± 0.004	0.436TrpPro: 0.436 ± 0.003	0.511TrpGln: 0.511 ± 0.004	0.756TrpArg: 0.756 ± 0.004	0.98TrpSer: 0.98 ± 0.005	0.73TrpThr: 0.73 ± 0.006	0.701TrpVal: 0.701 ± 0.005	0.21TrpTrp: 0.21 ± 0.002	0.369TrpTyr: 0.369 ± 0.003	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.49TyrAla: 1.49 ± 0.007	0.75TyrCys: 0.75 ± 0.005	1.467TyrAsp: 1.467 ± 0.006	1.679TyrGlu: 1.679 ± 0.007	1.271TyrPhe: 1.271 ± 0.006	1.747TyrGly: 1.747 ± 0.008	0.814TyrHis: 0.814 ± 0.005	1.595TyrIle: 1.595 ± 0.008	1.547TyrLys: 1.547 ± 0.007	2.753TyrLeu: 2.753 ± 0.008	0.72TyrMet: 0.72 ± 0.005	1.275TyrAsn: 1.275 ± 0.005	1.318TyrPro: 1.318 ± 0.007	1.289TyrGln: 1.289 ± 0.006	1.734TyrArg: 1.734 ± 0.006	2.384TyrSer: 2.384 ± 0.009	1.744TyrThr: 1.744 ± 0.008	1.674TyrVal: 1.674 ± 0.006	0.422TyrTrp: 0.422 ± 0.004	1.031TyrTyr: 1.031 ± 0.006	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.064XaaXaa: 0.064 ± 0.012

Statistics based on 69200 proteins (47700975 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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