Providence virus (isolate Helicoverpa zea/United States/vFLM1/-) (PRVFL)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Carmotetraviridae; Alphacarmotetravirus; Providence virus

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q80IX5|Q80IX5_PRVFL p81 OS=Providence virus (isolate Helicoverpa zea/United States/vFLM1/-) OX=1289469 PE=1 SV=1
MM1 pKa = 7.37QNLPVPNGEE10 pKa = 4.65SPPKK14 pKa = 8.88TDD16 pKa = 2.84EE17 pKa = 4.06VRR19 pKa = 11.84DD20 pKa = 3.69SRR22 pKa = 11.84RR23 pKa = 11.84NSDD26 pKa = 3.81DD27 pKa = 3.85EE28 pKa = 4.15EE29 pKa = 5.41PEE31 pKa = 4.13YY32 pKa = 10.67PRR34 pKa = 11.84GDD36 pKa = 4.23PIEE39 pKa = 5.5DD40 pKa = 3.59LTDD43 pKa = 4.3DD44 pKa = 4.72GDD46 pKa = 3.81IEE48 pKa = 4.72KK49 pKa = 11.07NPGPASANQAGKK61 pKa = 10.31RR62 pKa = 11.84LEE64 pKa = 3.85PRR66 pKa = 11.84KK67 pKa = 8.37RR68 pKa = 11.84TRR70 pKa = 11.84SIRR73 pKa = 11.84SRR75 pKa = 11.84QDD77 pKa = 3.18LDD79 pKa = 3.28TMHH82 pKa = 6.88RR83 pKa = 11.84VVVSVDD89 pKa = 3.03VDD91 pKa = 3.82SLTTLMGNIMTLAGSGGRR109 pKa = 11.84GSLLTAGDD117 pKa = 3.84VEE119 pKa = 4.97KK120 pKa = 11.35NPGPQMTRR128 pKa = 11.84EE129 pKa = 3.84NKK131 pKa = 9.57APNMSVRR138 pKa = 11.84AQNVTVSNSRR148 pKa = 11.84RR149 pKa = 11.84RR150 pKa = 11.84RR151 pKa = 11.84RR152 pKa = 11.84KK153 pKa = 8.25NRR155 pKa = 11.84KK156 pKa = 7.54PRR158 pKa = 11.84KK159 pKa = 8.38QVEE162 pKa = 3.88QATILKK168 pKa = 8.76PQLLPGEE175 pKa = 4.5SVAPSGGRR183 pKa = 11.84GTMDD187 pKa = 3.57PPVHH191 pKa = 6.63EE192 pKa = 5.12ICAQSIDD199 pKa = 3.88PSEE202 pKa = 4.37GAVGWFYY209 pKa = 11.64KK210 pKa = 11.03YY211 pKa = 9.53MDD213 pKa = 4.17PAGAVEE219 pKa = 4.25SGKK222 pKa = 10.73ALGEE226 pKa = 3.96FSKK229 pKa = 11.34VPDD232 pKa = 3.53GLLRR236 pKa = 11.84YY237 pKa = 9.91SVDD240 pKa = 3.15AEE242 pKa = 3.78QRR244 pKa = 11.84PIVTIEE250 pKa = 4.18CPTVSEE256 pKa = 4.69SDD258 pKa = 3.65LPLDD262 pKa = 3.88GKK264 pKa = 10.02LWRR267 pKa = 11.84VSFISFPAFRR277 pKa = 11.84LNFIALANINNEE289 pKa = 3.79ALTLEE294 pKa = 4.27TRR296 pKa = 11.84NTFIQTLNNITNWRR310 pKa = 11.84DD311 pKa = 3.0LGTGQWAQFAPGWYY325 pKa = 8.96YY326 pKa = 10.96SIYY329 pKa = 10.55VLPNTYY335 pKa = 11.18AMAEE339 pKa = 3.87IGDD342 pKa = 4.01RR343 pKa = 11.84TDD345 pKa = 3.43SVTQFRR351 pKa = 11.84KK352 pKa = 9.02VYY354 pKa = 10.37KK355 pKa = 10.72GITFEE360 pKa = 4.91FNAPTLIDD368 pKa = 3.42QGWWVGAHH376 pKa = 6.22IPVKK380 pKa = 9.47PQSEE384 pKa = 4.68TIPAAEE390 pKa = 4.17RR391 pKa = 11.84FSAGSMTVSASNAIFQPSNTVARR414 pKa = 11.84IVWSITPLPVATVALTTGTGGTNNTSGKK442 pKa = 9.34FFSVEE447 pKa = 2.89IDD449 pKa = 3.62GNVNSVWTFTAPASILAEE467 pKa = 3.9GEE469 pKa = 4.09PFAEE473 pKa = 4.67EE474 pKa = 4.32GDD476 pKa = 3.86TTSFSMTTITADD488 pKa = 3.4TVVYY492 pKa = 10.14SVSSSLTGSSVIVRR506 pKa = 11.84GVTKK510 pKa = 10.64GSGVSITPVTVGIDD524 pKa = 3.42TEE526 pKa = 4.4AVNRR530 pKa = 11.84LSIEE534 pKa = 4.1MPALTTEE541 pKa = 4.34EE542 pKa = 4.27VTTNVPKK549 pKa = 10.94YY550 pKa = 9.86EE551 pKa = 3.92QFLCKK556 pKa = 10.06EE557 pKa = 4.0SGGAYY562 pKa = 8.28IVHH565 pKa = 6.29YY566 pKa = 10.69KK567 pKa = 9.43MNNPVFEE574 pKa = 4.34MTGEE578 pKa = 4.05EE579 pKa = 4.14NFGGFQFHH587 pKa = 6.58YY588 pKa = 10.08PGYY591 pKa = 10.35DD592 pKa = 3.35PEE594 pKa = 4.94NNALGLRR601 pKa = 11.84GIVDD605 pKa = 3.41TFEE608 pKa = 5.34NNFSSAVVHH617 pKa = 6.46FWGISQSATIVCKK630 pKa = 10.02TYY632 pKa = 10.82DD633 pKa = 3.23GWEE636 pKa = 4.15GTTNAGSTVGQFAHH650 pKa = 6.62TGAEE654 pKa = 4.09EE655 pKa = 3.88EE656 pKa = 4.66DD657 pKa = 4.07EE658 pKa = 4.58VVQLANRR665 pKa = 11.84LQMEE669 pKa = 4.59LTGVYY674 pKa = 9.74QADD677 pKa = 3.83DD678 pKa = 3.77NFAGTVSALASIGLGLLGKK697 pKa = 10.38SSATPSVIKK706 pKa = 10.73GIAQQAVGAVQANPGILEE724 pKa = 4.26GAVKK728 pKa = 10.79AIGSVGARR736 pKa = 11.84LVGSIKK742 pKa = 10.27ARR744 pKa = 11.84RR745 pKa = 11.84ARR747 pKa = 11.84RR748 pKa = 11.84RR749 pKa = 11.84ARR751 pKa = 11.84RR752 pKa = 11.84AKK754 pKa = 10.36

Molecular weight: 81.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q80IX5|Q80IX5_PRVFL p81 OS=Providence virus (isolate Helicoverpa zea/United States/vFLM1/-) OX=1289469 PE=1 SV=1
MM1 pKa = 7.85CEE3 pKa = 3.99PLSLRR8 pKa = 11.84LCISCKK14 pKa = 9.77ATTSSSEE21 pKa = 3.97SRR23 pKa = 11.84GEE25 pKa = 3.9EE26 pKa = 3.88LLSGSKK32 pKa = 10.17SPPPPKK38 pKa = 9.96PSLTQTLLRR47 pKa = 11.84YY48 pKa = 9.33LSSMLTHH55 pKa = 7.02LGAAIMSPLSVVTGRR70 pKa = 11.84IIPSGNTILRR80 pKa = 11.84ASLALTSATCFYY92 pKa = 10.22THH94 pKa = 6.65LRR96 pKa = 11.84GYY98 pKa = 10.47RR99 pKa = 11.84YY100 pKa = 9.53ILRR103 pKa = 11.84FCRR106 pKa = 11.84TDD108 pKa = 3.45LVLFTQSVLNLLLQRR123 pKa = 11.84IGLKK127 pKa = 8.89SLRR130 pKa = 11.84LSLDD134 pKa = 3.32TTISLPFVIPQFVNIVDD151 pKa = 3.85YY152 pKa = 11.14AQLSQALQHH161 pKa = 5.98TRR163 pKa = 11.84THH165 pKa = 5.48TRR167 pKa = 11.84SATFLNVSHH176 pKa = 7.26IPDD179 pKa = 3.2QLLRR183 pKa = 11.84FAEE186 pKa = 4.4KK187 pKa = 10.23SWIRR191 pKa = 11.84QQLRR195 pKa = 11.84HH196 pKa = 5.74VDD198 pKa = 3.43LMGPAEE204 pKa = 4.25VVEE207 pKa = 4.74FQHH210 pKa = 6.14WLSNRR215 pKa = 11.84LVDD218 pKa = 6.29LYY220 pKa = 9.47PTWRR224 pKa = 11.84PLLAALGLIALAWCLTPVARR244 pKa = 11.84VLLEE248 pKa = 4.05EE249 pKa = 5.05GEE251 pKa = 4.39EE252 pKa = 3.97KK253 pKa = 11.11DD254 pKa = 3.46PVVRR258 pKa = 11.84NLTAVLRR265 pKa = 11.84SHH267 pKa = 7.66AIFCHH272 pKa = 5.23RR273 pKa = 11.84TAGTRR278 pKa = 11.84RR279 pKa = 11.84MLADD283 pKa = 4.24RR284 pKa = 11.84ASAWLRR290 pKa = 11.84KK291 pKa = 7.46EE292 pKa = 3.98HH293 pKa = 6.87PNMEE297 pKa = 4.27EE298 pKa = 3.77HH299 pKa = 6.4MKK301 pKa = 9.9PALVARR307 pKa = 11.84AVALAMIPCRR317 pKa = 11.84EE318 pKa = 4.09EE319 pKa = 3.85VEE321 pKa = 4.81SLIDD325 pKa = 3.54QQKK328 pKa = 10.7NSASLWWVTNLLRR341 pKa = 11.84SGWNSPWEE349 pKa = 3.96WLLGRR354 pKa = 11.84TIPTKK359 pKa = 10.72

Molecular weight: 40.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3248

359

1220

812.0

89.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.22 ± 0.599

1.817 ± 0.341

3.972 ± 0.229

5.45 ± 0.242

2.494 ± 0.375

7.512 ± 1.601

2.679 ± 0.479

3.941 ± 0.703

3.725 ± 0.131

9.483 ± 2.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.97 ± 0.573

3.756 ± 0.45

6.435 ± 0.206

4.126 ± 0.195

7.112 ± 0.363

8.621 ± 0.429

6.866 ± 0.437

7.574 ± 0.434

1.817 ± 0.425

2.401 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski