Eggerthella sp. CAG:298

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Eggerthella; environmental samples

Average proteome isoelectric point is 5.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1581 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7B5E6|R7B5E6_9ACTN CRISPR-associated endonuclease Cas1 OS=Eggerthella sp. CAG:298 OX=1262876 GN=cas1 PE=3 SV=1
MM1 pKa = 7.43KK2 pKa = 10.21KK3 pKa = 9.77ISKK6 pKa = 9.95AKK8 pKa = 10.08NSFVAHH14 pKa = 6.94AIVVAVSLFLVAFSLSAPGVAFAEE38 pKa = 4.33DD39 pKa = 3.94VEE41 pKa = 4.79VQSSEE46 pKa = 4.0PTQTLIDD53 pKa = 3.9QPAVAAEE60 pKa = 3.85ATPAVEE66 pKa = 4.15AVSVPSDD73 pKa = 3.39PAQVSGVEE81 pKa = 4.02AVPGNSSTQKK91 pKa = 10.65SEE93 pKa = 4.14GASSALNNDD102 pKa = 2.78KK103 pKa = 9.28NTNVEE108 pKa = 4.08NSASDD113 pKa = 3.56PAASNDD119 pKa = 3.49SNATEE124 pKa = 4.58FSTTQVEE131 pKa = 4.65EE132 pKa = 3.99KK133 pKa = 10.43ATIKK137 pKa = 10.26PAADD141 pKa = 3.27SPHH144 pKa = 6.27RR145 pKa = 11.84VNLSFKK151 pKa = 10.25ILYY154 pKa = 9.28QGQEE158 pKa = 3.82FTDD161 pKa = 5.09GYY163 pKa = 10.81EE164 pKa = 4.01IGHH167 pKa = 6.28SDD169 pKa = 3.3SVSFVCTMGDD179 pKa = 3.28SHH181 pKa = 7.79ASTAASHH188 pKa = 6.48MISMGDD194 pKa = 3.39VLIEE198 pKa = 3.88RR199 pKa = 11.84DD200 pKa = 3.65ALAKK204 pKa = 9.83FLRR207 pKa = 11.84EE208 pKa = 4.16GYY210 pKa = 9.75EE211 pKa = 3.46IRR213 pKa = 11.84GWTKK217 pKa = 10.74SLLAGAEE224 pKa = 4.16VYY226 pKa = 10.87GFDD229 pKa = 4.81RR230 pKa = 11.84DD231 pKa = 4.72GIEE234 pKa = 4.36CMDD237 pKa = 4.16GEE239 pKa = 4.73TIYY242 pKa = 10.48IVATYY247 pKa = 10.42GPAANQFTVNFDD259 pKa = 3.32AMGGSNAPEE268 pKa = 4.23SLTEE272 pKa = 3.78VSTEE276 pKa = 3.65NEE278 pKa = 4.1YY279 pKa = 9.71TFTISDD285 pKa = 3.8AVPTRR290 pKa = 11.84EE291 pKa = 4.2GYY293 pKa = 10.94NFVGWSTNSSLLPPYY308 pKa = 10.18LKK310 pKa = 10.84AGDD313 pKa = 3.67TVTVSEE319 pKa = 4.27NDD321 pKa = 3.24SYY323 pKa = 11.89AITLYY328 pKa = 10.87AVWEE332 pKa = 4.12IANKK336 pKa = 10.05VEE338 pKa = 4.0LVYY341 pKa = 10.95AVDD344 pKa = 3.9YY345 pKa = 10.91VQFGDD350 pKa = 4.02AEE352 pKa = 4.6TYY354 pKa = 10.39AQGSSAVVKK363 pKa = 10.44GCDD366 pKa = 3.65YY367 pKa = 11.31VKK369 pKa = 10.56EE370 pKa = 4.49GYY372 pKa = 10.42KK373 pKa = 10.52FIGWSLDD380 pKa = 3.14PMSDD384 pKa = 2.85HH385 pKa = 7.37ASYY388 pKa = 11.29KK389 pKa = 10.45EE390 pKa = 3.88GDD392 pKa = 4.24SIIMDD397 pKa = 3.92STSGSIWLYY406 pKa = 9.52AVWVRR411 pKa = 11.84TYY413 pKa = 10.51TVTYY417 pKa = 10.02TDD419 pKa = 3.81GQGGTVFEE427 pKa = 4.32NQVISGVEE435 pKa = 4.19SGTSTPTFPVSRR447 pKa = 11.84PSSIVIDD454 pKa = 3.64GNTYY458 pKa = 10.21VFSGWDD464 pKa = 3.3NNAEE468 pKa = 4.17VVLGDD473 pKa = 4.1MIINAIWKK481 pKa = 9.78LDD483 pKa = 3.66ANGNGVADD491 pKa = 3.77EE492 pKa = 5.07DD493 pKa = 3.89EE494 pKa = 5.03AKK496 pKa = 10.84FSVTYY501 pKa = 9.59TDD503 pKa = 3.93GVGGTAFGDD512 pKa = 3.91YY513 pKa = 10.53VISNLLVNTATPRR526 pKa = 11.84YY527 pKa = 8.14RR528 pKa = 11.84APEE531 pKa = 3.55RR532 pKa = 11.84AGYY535 pKa = 9.47IFQGWSPTVADD546 pKa = 5.18IITGNAIYY554 pKa = 8.87TAIWAEE560 pKa = 4.02DD561 pKa = 3.52KK562 pKa = 11.27NNNNIPDD569 pKa = 4.21SEE571 pKa = 4.49EE572 pKa = 3.54EE573 pKa = 4.41TYY575 pKa = 10.58TIIFINDD582 pKa = 3.0VTGATLQTTPGVLGGLDD599 pKa = 3.48TPQFAGPAPTMTGYY613 pKa = 10.98VFVGWSPALAQTVDD627 pKa = 3.47PATADD632 pKa = 3.32EE633 pKa = 4.99FGNIYY638 pKa = 10.45YY639 pKa = 9.97IARR642 pKa = 11.84WEE644 pKa = 3.78VDD646 pKa = 2.99ANNNGIADD654 pKa = 3.75VDD656 pKa = 3.67EE657 pKa = 4.58ARR659 pKa = 11.84YY660 pKa = 9.94SVIYY664 pKa = 10.48SDD666 pKa = 4.02GANGSVFADD675 pKa = 3.39QTTTGLLSGLATPQFTGEE693 pKa = 4.1TPVRR697 pKa = 11.84SGYY700 pKa = 10.09IFAGWSSSPAGTIDD714 pKa = 3.3PTAADD719 pKa = 3.36SLGRR723 pKa = 11.84IIYY726 pKa = 6.66TALWAADD733 pKa = 3.51VNGNGVADD741 pKa = 4.1SEE743 pKa = 4.65EE744 pKa = 3.92PTYY747 pKa = 10.73TITYY751 pKa = 8.76TDD753 pKa = 3.94GANGAAFADD762 pKa = 4.13QVSSGLLGGVATPQFSGTPTRR783 pKa = 11.84TGYY786 pKa = 11.15KK787 pKa = 9.15FVGWSTEE794 pKa = 3.86PASTVSGDD802 pKa = 3.78VVYY805 pKa = 9.1TALWEE810 pKa = 4.2AVEE813 pKa = 4.42PDD815 pKa = 4.21PTPGTDD821 pKa = 5.1DD822 pKa = 3.58PTPTPGPDD830 pKa = 3.84DD831 pKa = 4.26PAPTPGDD838 pKa = 4.13DD839 pKa = 5.3DD840 pKa = 6.33DD841 pKa = 4.19NTGGNGSATTPGTNEE856 pKa = 4.33PGNVTPNPGPANGTTTPGEE875 pKa = 4.01TTIADD880 pKa = 4.29DD881 pKa = 4.06EE882 pKa = 4.79TPLAGGAGEE891 pKa = 4.6TEE893 pKa = 4.53DD894 pKa = 5.61DD895 pKa = 3.97PAEE898 pKa = 4.39GDD900 pKa = 3.9VADD903 pKa = 4.94DD904 pKa = 3.95AAVADD909 pKa = 4.6EE910 pKa = 4.32AAGAIDD916 pKa = 3.75EE917 pKa = 4.64AADD920 pKa = 3.72TEE922 pKa = 4.4ILDD925 pKa = 5.18DD926 pKa = 4.56EE927 pKa = 4.98NPLAAADD934 pKa = 4.78HH935 pKa = 6.07SSSWLSWLLTVGLLLVVCFGVFFLVRR961 pKa = 11.84RR962 pKa = 11.84RR963 pKa = 11.84KK964 pKa = 7.85NTRR967 pKa = 11.84EE968 pKa = 3.78QNQQ971 pKa = 3.04

Molecular weight: 102.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7B7V5|R7B7V5_9ACTN Membrane-flanked domain protein OS=Eggerthella sp. CAG:298 OX=1262876 GN=BN592_00099 PE=4 SV=1
MM1 pKa = 7.48LSKK4 pKa = 10.58DD5 pKa = 3.66ARR7 pKa = 11.84AQPMNRR13 pKa = 11.84SNPGVFDD20 pKa = 3.62HH21 pKa = 6.76EE22 pKa = 4.36RR23 pKa = 11.84LFIEE27 pKa = 4.88ALLLKK32 pKa = 10.24KK33 pKa = 9.22RR34 pKa = 11.84THH36 pKa = 6.08ARR38 pKa = 11.84FHH40 pKa = 6.43LSCTRR45 pKa = 11.84FAEE48 pKa = 4.85GSRR51 pKa = 11.84KK52 pKa = 9.81YY53 pKa = 10.69FIDD56 pKa = 5.26AIDD59 pKa = 3.47EE60 pKa = 4.67RR61 pKa = 11.84IFRR64 pKa = 11.84ARR66 pKa = 11.84FTHH69 pKa = 6.75

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1581

0

1581

518525

35

4679

328.0

35.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.414 ± 0.065

1.481 ± 0.028

5.883 ± 0.054

7.132 ± 0.069

4.029 ± 0.041

7.344 ± 0.06

1.978 ± 0.032

6.316 ± 0.053

4.513 ± 0.051

9.3 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.031

3.522 ± 0.043

4.179 ± 0.033

3.252 ± 0.029

5.076 ± 0.053

6.049 ± 0.054

5.552 ± 0.073

7.512 ± 0.054

0.939 ± 0.021

2.953 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski