Gynuella sunshinyii YC6258

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Saccharospirillaceae; Gynuella; Gynuella sunshinyii

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5913 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5VHJ8|A0A0C5VHJ8_9GAMM Uncharacterized protein OS=Gynuella sunshinyii YC6258 OX=1445510 GN=YC6258_02106 PE=4 SV=1
MM1 pKa = 7.14THH3 pKa = 6.4QKK5 pKa = 10.9LLLTAACFSIVTLTACNSSDD25 pKa = 4.98SGDD28 pKa = 3.34SDD30 pKa = 3.47NFKK33 pKa = 11.12RR34 pKa = 11.84NLQMNLSDD42 pKa = 3.87STTQALLDD50 pKa = 3.93DD51 pKa = 4.36TLATNAGTTTSLSIAQGDD69 pKa = 4.04LSADD73 pKa = 3.89EE74 pKa = 3.96ISIRR78 pKa = 11.84SLNNSSQVLARR89 pKa = 11.84KK90 pKa = 7.19MARR93 pKa = 11.84TAFNQNDD100 pKa = 3.37KK101 pKa = 10.58SACDD105 pKa = 3.45SGSVSVSISGDD116 pKa = 3.09IADD119 pKa = 5.46DD120 pKa = 3.72EE121 pKa = 4.78TSGNVTAALVASNCRR136 pKa = 11.84SYY138 pKa = 11.3EE139 pKa = 3.86SSDD142 pKa = 3.71GINIEE147 pKa = 3.98GMINGSLSDD156 pKa = 3.69SVSWQTSGSQFTALSSTSTGNLEE179 pKa = 3.51ITYY182 pKa = 10.46VGDD185 pKa = 3.92GSSDD189 pKa = 2.88GHH191 pKa = 6.1NYY193 pKa = 10.42SFGFLYY199 pKa = 10.76DD200 pKa = 4.82LSDD203 pKa = 4.31LNNSMSLNTVNNTLEE218 pKa = 3.62VDD220 pKa = 3.46ANYY223 pKa = 10.84SVYY226 pKa = 10.46IEE228 pKa = 4.57VYY230 pKa = 10.63YY231 pKa = 9.81EE232 pKa = 3.74TDD234 pKa = 3.1NDD236 pKa = 3.74KK237 pKa = 11.24EE238 pKa = 4.21RR239 pKa = 11.84VGGSMKK245 pKa = 10.4VATLDD250 pKa = 3.5SVIYY254 pKa = 9.59DD255 pKa = 3.7APTTTSSLSHH265 pKa = 6.5PVDD268 pKa = 3.06GRR270 pKa = 11.84IQVTAGGQTATVDD283 pKa = 4.67FDD285 pKa = 3.54QTDD288 pKa = 3.72YY289 pKa = 11.18TVTIGNVSNTYY300 pKa = 10.3DD301 pKa = 3.84YY302 pKa = 11.75EE303 pKa = 6.58DD304 pKa = 3.62DD305 pKa = 3.93TVFF308 pKa = 4.52

Molecular weight: 32.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5VDW0|A0A0C5VDW0_9GAMM Transport permease protein OS=Gynuella sunshinyii YC6258 OX=1445510 GN=YC6258_00346 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.9GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5913

0

5913

1865711

37

13859

315.5

35.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.577 ± 0.055

1.089 ± 0.013

5.782 ± 0.046

5.838 ± 0.036

4.03 ± 0.027

7.008 ± 0.045

2.447 ± 0.018

6.184 ± 0.036

4.17 ± 0.049

10.397 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.525 ± 0.025

4.055 ± 0.032

4.173 ± 0.03

5.012 ± 0.038

5.195 ± 0.027

6.706 ± 0.033

5.436 ± 0.038

6.727 ± 0.027

1.447 ± 0.015

3.203 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski