Gabek Forest virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Gabek phlebovirus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8JX57|W8JX57_9VIRU Nonstructural protein OS=Gabek Forest virus OX=629736 PE=4 SV=1
MM1 pKa = 6.63TTRR4 pKa = 11.84FLYY7 pKa = 10.55DD8 pKa = 3.18RR9 pKa = 11.84LYY11 pKa = 10.62IMQSEE16 pKa = 4.59GQKK19 pKa = 10.17LQAVFLAHH27 pKa = 6.49NNFVGDD33 pKa = 3.96DD34 pKa = 3.69VSSYY38 pKa = 11.85DD39 pKa = 3.22HH40 pKa = 6.59MEE42 pKa = 4.2VVLSKK47 pKa = 10.63YY48 pKa = 9.88KK49 pKa = 10.5QSFDD53 pKa = 3.56YY54 pKa = 10.96RR55 pKa = 11.84EE56 pKa = 4.19SLSDD60 pKa = 3.96FYY62 pKa = 11.82SRR64 pKa = 11.84GEE66 pKa = 3.87LPFRR70 pKa = 11.84WGSIMWCSRR79 pKa = 11.84VTGEE83 pKa = 4.53EE84 pKa = 4.15YY85 pKa = 10.83LSFMALMMDD94 pKa = 4.59LLKK97 pKa = 10.69LDD99 pKa = 3.61SSEE102 pKa = 4.66LKK104 pKa = 10.74GDD106 pKa = 3.21EE107 pKa = 4.31HH108 pKa = 7.29PNIRR112 pKa = 11.84EE113 pKa = 3.99ALSWPTGSPTLGFIKK128 pKa = 10.36LNCRR132 pKa = 11.84SSDD135 pKa = 2.82WSINFQKK142 pKa = 10.77SRR144 pKa = 11.84TATLLLRR151 pKa = 11.84AGGSDD156 pKa = 3.32EE157 pKa = 4.15GLEE160 pKa = 3.89EE161 pKa = 5.92AIVKK165 pKa = 6.04THH167 pKa = 6.23KK168 pKa = 10.38KK169 pKa = 10.02IIMEE173 pKa = 3.93SSIRR177 pKa = 11.84GFDD180 pKa = 3.29PKK182 pKa = 10.63FFPGLDD188 pKa = 4.19LIRR191 pKa = 11.84EE192 pKa = 4.38VACLQCVRR200 pKa = 11.84LMNASCFDD208 pKa = 4.18TVHH211 pKa = 6.04TAYY214 pKa = 9.95PSRR217 pKa = 11.84LLDD220 pKa = 3.81LLAMHH225 pKa = 7.41RR226 pKa = 11.84STYY229 pKa = 10.14TMISSKK235 pKa = 10.93LLGNRR240 pKa = 11.84KK241 pKa = 5.11WTPVQGTHH249 pKa = 6.48FDD251 pKa = 4.05EE252 pKa = 5.52PDD254 pKa = 2.99ATYY257 pKa = 11.05SFSSDD262 pKa = 3.14SEE264 pKa = 4.24

Molecular weight: 30.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8JDL6|W8JDL6_9VIRU Membrane glycoprotein OS=Gabek Forest virus OX=629736 PE=4 SV=1
MM1 pKa = 7.7SDD3 pKa = 3.29YY4 pKa = 11.22AQIAIEE10 pKa = 4.22FGNSEE15 pKa = 3.88VDD17 pKa = 3.18GDD19 pKa = 5.52LIEE22 pKa = 4.9AWVKK26 pKa = 9.49EE27 pKa = 3.89FEE29 pKa = 4.46YY30 pKa = 10.74QGFDD34 pKa = 3.18PRR36 pKa = 11.84IVVKK40 pKa = 10.49LVSEE44 pKa = 4.4VEE46 pKa = 4.14GWQTDD51 pKa = 3.73VKK53 pKa = 11.29KK54 pKa = 10.46MIILALTRR62 pKa = 11.84GNKK65 pKa = 7.72PEE67 pKa = 3.99KK68 pKa = 9.83MVTKK72 pKa = 9.73MSAKK76 pKa = 9.86GRR78 pKa = 11.84EE79 pKa = 4.02EE80 pKa = 3.75VAKK83 pKa = 10.47LVKK86 pKa = 10.05KK87 pKa = 10.85YY88 pKa = 10.36KK89 pKa = 10.34LKK91 pKa = 10.78SGNPGRR97 pKa = 11.84NDD99 pKa = 3.09LTLSRR104 pKa = 11.84VAAAFASWTCNAIYY118 pKa = 9.89HH119 pKa = 4.86VQYY122 pKa = 10.51YY123 pKa = 10.51LPVTGNHH130 pKa = 5.71MDD132 pKa = 5.16AISKK136 pKa = 9.78NYY138 pKa = 7.95PRR140 pKa = 11.84PMMHH144 pKa = 7.22PSFAGLIDD152 pKa = 3.45PAMRR156 pKa = 11.84DD157 pKa = 3.37RR158 pKa = 11.84QILIEE163 pKa = 4.05AHH165 pKa = 6.27SLFLIEE171 pKa = 5.02FAQKK175 pKa = 10.53INVNLRR181 pKa = 11.84GKK183 pKa = 10.12SKK185 pKa = 11.49AEE187 pKa = 3.45ICLSFDD193 pKa = 3.33QPLNAAINSNFLTSAQKK210 pKa = 10.75LKK212 pKa = 11.04ALTALGIIDD221 pKa = 4.76DD222 pKa = 4.14HH223 pKa = 6.6QKK225 pKa = 10.29PSKK228 pKa = 10.69DD229 pKa = 3.5VVNAADD235 pKa = 3.4AFRR238 pKa = 11.84RR239 pKa = 11.84II240 pKa = 3.89

Molecular weight: 26.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

4034

240

2102

1008.5

113.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.801 ± 0.636

2.553 ± 0.594

5.156 ± 0.522

7.412 ± 0.494

4.388 ± 0.589

6.197 ± 0.255

2.305 ± 0.121

6.916 ± 0.56

6.743 ± 0.5

8.528 ± 0.467

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.123 ± 0.45

4.09 ± 0.415

3.867 ± 0.197

2.876 ± 0.302

5.578 ± 0.227

9.048 ± 0.402

5.751 ± 0.595

5.925 ± 0.243

1.19 ± 0.158

2.553 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski