Brevirhabdus pacifica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobactera

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2900 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7DIN3|A0A1U7DIN3_9RHOB 23S rRNA m(5)U-1939 methyltransferase OS=Brevirhabdus pacifica OX=1267768 GN=BV394_09060 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 10.22KK3 pKa = 10.39VLFASTALVATAGVAAAEE21 pKa = 4.33VAVSGKK27 pKa = 10.74AEE29 pKa = 3.75MGIFGGTGIEE39 pKa = 4.12TQFFQDD45 pKa = 3.17LDD47 pKa = 3.85VTFGMSGTTDD57 pKa = 2.91NGLSFGASIDD67 pKa = 5.06LDD69 pKa = 3.97EE70 pKa = 4.88SWQGTNGAAPNGALANNADD89 pKa = 4.54DD90 pKa = 4.41GGASIFVSGDD100 pKa = 2.92FGTLTMGDD108 pKa = 3.38TDD110 pKa = 5.21GALDD114 pKa = 3.47WAMTEE119 pKa = 4.27AGNVGNPGSINDD131 pKa = 4.99DD132 pKa = 3.76EE133 pKa = 4.67TSHH136 pKa = 7.11AGYY139 pKa = 10.39LGAYY143 pKa = 9.98LDD145 pKa = 4.08GSYY148 pKa = 10.76DD149 pKa = 3.83GQIVRR154 pKa = 11.84YY155 pKa = 9.43DD156 pKa = 3.3NTYY159 pKa = 10.84GDD161 pKa = 3.57FGFAVSIEE169 pKa = 4.35ADD171 pKa = 3.24DD172 pKa = 3.78TAPVRR177 pKa = 11.84NVGYY181 pKa = 10.63AVGVKK186 pKa = 10.46YY187 pKa = 10.9NLDD190 pKa = 3.64LGGTTLNLGVGHH202 pKa = 5.67QQATLAAGEE211 pKa = 4.22TSQTGISVGTKK222 pKa = 8.18MAAGVSAGVTYY233 pKa = 11.18TMMNDD238 pKa = 2.61IGGAAGVDD246 pKa = 3.75GKK248 pKa = 10.44HH249 pKa = 6.04IGVGVGYY256 pKa = 10.22SAGAVSLHH264 pKa = 6.21ANYY267 pKa = 10.49GQFDD271 pKa = 4.49PDD273 pKa = 3.54NAGKK277 pKa = 8.82TSGYY281 pKa = 10.29GLAAGYY287 pKa = 10.47DD288 pKa = 3.49LGGGLKK294 pKa = 10.48LLAGYY299 pKa = 9.29GKK301 pKa = 10.01NEE303 pKa = 4.01PAAGASTDD311 pKa = 3.47MYY313 pKa = 11.4SFGASMSFF321 pKa = 3.33

Molecular weight: 32.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7DJP1|A0A1U7DJP1_9RHOB Anti-anti-sigma factor OS=Brevirhabdus pacifica OX=1267768 GN=BV394_11020 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2900

0

2900

930718

41

1941

320.9

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.711 ± 0.052

0.849 ± 0.015

6.022 ± 0.036

6.235 ± 0.048

3.492 ± 0.028

9.095 ± 0.052

2.004 ± 0.024

4.791 ± 0.029

2.838 ± 0.039

10.188 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.024

2.387 ± 0.023

5.339 ± 0.03

2.995 ± 0.024

7.603 ± 0.044

4.971 ± 0.031

5.214 ± 0.028

7.182 ± 0.034

1.324 ± 0.021

2.056 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski