bacterium J17

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 527 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A202DUX6|A0A202DUX6_9BACT Uncharacterized protein OS=bacterium J17 OX=1932695 GN=BVY02_02200 PE=4 SV=1
KKK2 pKa = 10.07KK3 pKa = 10.55NDDD6 pKa = 4.06AVGARR11 pKa = 11.84TDDD14 pKa = 3.35DDD16 pKa = 3.54GSGNGAVYYY25 pKa = 10.35LFLNTTGVVDDD36 pKa = 4.5YYY38 pKa = 11.81KKK40 pKa = 10.67SDDD43 pKa = 3.91EEE45 pKa = 4.35SFTAILDDD53 pKa = 3.74GQFGMSVTNTGDDD66 pKa = 3.27DDD68 pKa = 3.74DDD70 pKa = 3.97VNDDD74 pKa = 4.64GVGAYYY80 pKa = 10.17DDD82 pKa = 5.8DD83 pKa = 5.71DD84 pKa = 4.42GDDD87 pKa = 3.38RR88 pKa = 11.84GAFYYY93 pKa = 11.17LFMQTTGTVSSFQKKK108 pKa = 10.77SDDD111 pKa = 3.56AGSFTATLDDD121 pKa = 3.25TDDD124 pKa = 3.67LGVSISNAGDDD135 pKa = 3.76DDD137 pKa = 4.2DDD139 pKa = 4.42VDDD142 pKa = 4.56EE143 pKa = 4.63ASGAWNDDD151 pKa = 4.72DD152 pKa = 3.4DD153 pKa = 6.03GTNRR157 pKa = 11.84GAFYYY162 pKa = 10.58LNLDDD167 pKa = 3.84PNGNSIGTLTINSHHH182 pKa = 6.74FLSIPTFSVSNNLTLTFTTEEE203 pKa = 3.51AIPANGDDD211 pKa = 2.54DDD213 pKa = 4.31IFPIGFDDD221 pKa = 3.36TSIGSSDDD229 pKa = 3.48SEEE232 pKa = 4.65GGDDD236 pKa = 3.57GTLTTSINGHHH247 pKa = 5.95MTINTGSATSASTQFALTILNLVNPVTVGTYYY279 pKa = 8.6TFLIEEE285 pKa = 4.24QDDD288 pKa = 3.47EE289 pKa = 4.38NAVIDDD295 pKa = 3.77GVGNAVDDD303 pKa = 4.12VSDDD307 pKa = 3.5YYY309 pKa = 11.11YYY311 pKa = 10.17EE312 pKa = 3.86SKKK315 pKa = 11.6EEE317 pKa = 4.82DD318 pKa = 4.07NNSYYY323 pKa = 10.97KKK325 pKa = 10.75DD326 pKa = 3.35DDD328 pKa = 3.8SLRR331 pKa = 11.84EEE333 pKa = 4.53ITAANAVGSAVTITFDDD350 pKa = 2.99TSCYYY355 pKa = 11.01STCVIAPTSTLPSLTSNYYY374 pKa = 9.54NIVGSSFLSGISRR387 pKa = 11.84HHH389 pKa = 5.66IFALVSCARR398 pKa = 11.84ICV

Molecular weight: 41.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A202DVV5|A0A202DVV5_9BACT Aminomethyltransferase OS=bacterium J17 OX=1932695 GN=gcvT PE=3 SV=1
MM1 pKa = 7.36SRR3 pKa = 11.84HH4 pKa = 4.91EE5 pKa = 4.56SNRR8 pKa = 11.84NRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 10.19NGGARR18 pKa = 11.84LSPTQRR24 pKa = 11.84SAHH27 pKa = 6.15LPLLTRR33 pKa = 11.84QKK35 pKa = 10.67EE36 pKa = 4.04LRR38 pKa = 11.84DD39 pKa = 3.61NEE41 pKa = 4.5LADD44 pKa = 4.69DD45 pKa = 4.26PLNRR49 pKa = 11.84IIAAEE54 pKa = 4.06KK55 pKa = 10.02ARR57 pKa = 11.84AGRR60 pKa = 11.84EE61 pKa = 3.81STINILMIFSSVGALVVWLGFFVLFQQIINLNWGEE96 pKa = 4.1GGQLMSRR103 pKa = 11.84RR104 pKa = 11.84VLDD107 pKa = 3.95GSSIILAQKK116 pKa = 7.57QHH118 pKa = 6.6PAAGGLSASNSLKK131 pKa = 9.92EE132 pKa = 4.0SGYY135 pKa = 8.54MRR137 pKa = 11.84GIVGEE142 pKa = 4.25VGTKK146 pKa = 7.99QVYY149 pKa = 8.74YY150 pKa = 9.93QAPPPQCLSHH160 pKa = 7.95LVMPII165 pKa = 2.98

Molecular weight: 18.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

527

0

527

156075

50

1856

296.2

32.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.427 ± 0.105

1.007 ± 0.044

5.365 ± 0.088

7.311 ± 0.116

4.55 ± 0.086

7.094 ± 0.099

1.674 ± 0.047

6.023 ± 0.071

5.56 ± 0.112

10.813 ± 0.133

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.858 ± 0.04

3.657 ± 0.078

4.061 ± 0.055

3.299 ± 0.057

5.429 ± 0.092

8.367 ± 0.109

4.564 ± 0.075

7.383 ± 0.121

0.948 ± 0.033

2.611 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski