Mycobacterium phage SirPhilip

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222ZLD7|A0A222ZLD7_9CAUD Head-to-tail adaptor OS=Mycobacterium phage SirPhilip OX=2015824 GN=13 PE=4 SV=1
MM1 pKa = 6.94TAPRR5 pKa = 11.84MGTTVEE11 pKa = 3.92DD12 pKa = 4.73AIALVEE18 pKa = 4.17AQTIGYY24 pKa = 8.01VQAFDD29 pKa = 3.44VALIVALSEE38 pKa = 4.1QVSDD42 pKa = 4.22DD43 pKa = 3.57GRR45 pKa = 11.84GAAYY49 pKa = 10.26SDD51 pKa = 4.01PAIQALAEE59 pKa = 4.15RR60 pKa = 11.84FDD62 pKa = 3.84EE63 pKa = 4.73VVAEE67 pKa = 4.26VAEE70 pKa = 4.45HH71 pKa = 6.91FGLTT75 pKa = 3.56

Molecular weight: 7.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222ZM77|A0A222ZM77_9CAUD Membrane protein OS=Mycobacterium phage SirPhilip OX=2015824 GN=79 PE=4 SV=1
MM1 pKa = 7.64CGAPANSTDD10 pKa = 4.72LFSKK14 pKa = 10.24ACGSARR20 pKa = 11.84VARR23 pKa = 11.84VRR25 pKa = 11.84TASKK29 pKa = 10.73NRR31 pKa = 11.84IVLASAQVKK40 pKa = 9.24YY41 pKa = 9.88PFRR44 pKa = 11.84VVSTDD49 pKa = 2.65STDD52 pKa = 2.82IYY54 pKa = 10.55RR55 pKa = 11.84LSSRR59 pKa = 11.84VEE61 pKa = 3.82IPTVSPGRR69 pKa = 11.84LSPSALCGADD79 pKa = 2.63IRR81 pKa = 11.84EE82 pKa = 4.45SVLSVLVSPVANAAGRR98 pKa = 11.84RR99 pKa = 11.84APP101 pKa = 3.65

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

19021

24

1301

196.1

21.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.164 ± 0.467

1.067 ± 0.14

6.566 ± 0.26

5.773 ± 0.352

2.629 ± 0.165

8.88 ± 0.486

2.229 ± 0.181

3.712 ± 0.166

3.254 ± 0.205

8.433 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.182 ± 0.123

2.639 ± 0.163

5.725 ± 0.24

3.002 ± 0.14

7.019 ± 0.391

5.231 ± 0.191

6.041 ± 0.282

8.017 ± 0.245

2.056 ± 0.102

2.382 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski