Microvirga tunisiensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Microvirga

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7944 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N7MX17|A0A5N7MX17_9RHIZ Transposase OS=Microvirga tunisiensis OX=2108360 GN=FS320_43330 PE=4 SV=1
MM1 pKa = 8.17DD2 pKa = 5.26SGDD5 pKa = 3.4ISVPGYY11 pKa = 10.17IWTGDD16 pKa = 3.42AGDD19 pKa = 3.77NAIAGTNGADD29 pKa = 3.44TMAGGAGNDD38 pKa = 3.58EE39 pKa = 4.35YY40 pKa = 11.13TVNHH44 pKa = 6.41SGDD47 pKa = 3.92LVTEE51 pKa = 4.17NAGEE55 pKa = 4.41GTDD58 pKa = 3.75HH59 pKa = 6.88VYY61 pKa = 11.25SSITYY66 pKa = 7.76TLTPNVEE73 pKa = 4.01NLTLTGTGAINGYY86 pKa = 10.89GNGLNNWIVGNAAVNTLKK104 pKa = 10.71SDD106 pKa = 4.5DD107 pKa = 4.65GDD109 pKa = 3.8DD110 pKa = 3.82TLDD113 pKa = 3.79GGGGADD119 pKa = 4.82SLDD122 pKa = 3.75GGNGNDD128 pKa = 3.4TYY130 pKa = 11.55YY131 pKa = 11.16VDD133 pKa = 4.74DD134 pKa = 4.38AADD137 pKa = 3.75VIVEE141 pKa = 4.21KK142 pKa = 10.85DD143 pKa = 3.37SLGLGGYY150 pKa = 8.92DD151 pKa = 3.12HH152 pKa = 6.97VFSSVSYY159 pKa = 9.33VLSAQVEE166 pKa = 4.52EE167 pKa = 4.37LTLTGSANLNATGNTGANVLNGNDD191 pKa = 4.04GDD193 pKa = 4.16NALDD197 pKa = 3.63GHH199 pKa = 6.54GGADD203 pKa = 3.48TMNGGLGNDD212 pKa = 3.76TYY214 pKa = 11.63YY215 pKa = 10.99VDD217 pKa = 3.94HH218 pKa = 7.19SGDD221 pKa = 3.7VVNEE225 pKa = 3.66AAGAGGGVDD234 pKa = 3.56TVISSVSISSTKK246 pKa = 9.84PLAANVEE253 pKa = 4.21NLVLTGLATEE263 pKa = 4.59GTGNGLDD270 pKa = 3.49NVITGRR276 pKa = 11.84PYY278 pKa = 9.17VRR280 pKa = 11.84CRR282 pKa = 11.84RR283 pKa = 11.84RR284 pKa = 11.84WLVHH288 pKa = 4.47TMRR291 pKa = 5.84

Molecular weight: 29.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N7MBB5|A0A5N7MBB5_9RHIZ Type II secretion system F family protein OS=Microvirga tunisiensis OX=2108360 GN=FS320_02615 PE=4 SV=1
MM1 pKa = 6.9MAAATSRR8 pKa = 11.84APPAAPALRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84ARR21 pKa = 11.84RR22 pKa = 11.84PAPARR27 pKa = 11.84PLRR30 pKa = 11.84LPRR33 pKa = 11.84RR34 pKa = 11.84LRR36 pKa = 11.84RR37 pKa = 11.84LPQPRR42 pKa = 11.84RR43 pKa = 11.84ALLPRR48 pKa = 11.84RR49 pKa = 11.84LRR51 pKa = 11.84PTRR54 pKa = 11.84SKK56 pKa = 10.09FASRR60 pKa = 11.84VTLRR64 pKa = 11.84TMGLTSGSGPFLRR77 pKa = 11.84GLPPGSS83 pKa = 3.41

Molecular weight: 9.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7944

0

7944

2184762

24

2831

275.0

30.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.651 ± 0.032

0.854 ± 0.01

5.519 ± 0.025

5.927 ± 0.028

3.612 ± 0.019

8.191 ± 0.028

2.147 ± 0.014

5.213 ± 0.019

3.397 ± 0.024

10.179 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.338 ± 0.013

2.618 ± 0.017

5.296 ± 0.02

3.44 ± 0.017

7.487 ± 0.031

5.765 ± 0.023

5.389 ± 0.024

7.397 ± 0.026

1.36 ± 0.01

2.22 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski