Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) (Kluyveromyces polysporus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Vanderwaltozyma; Vanderwaltozyma polyspora

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5353 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7TFA3|A7TFA3_VANPO Uncharacterized protein OS=Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) OX=436907 GN=Kpol_2000p96 PE=4 SV=1
MM1 pKa = 7.35QDD3 pKa = 3.29HH4 pKa = 7.03EE5 pKa = 4.39EE6 pKa = 5.02HH7 pKa = 6.93EE8 pKa = 5.25DD9 pKa = 4.22GPIDD13 pKa = 3.5QYY15 pKa = 11.66GSKK18 pKa = 10.68EE19 pKa = 3.99EE20 pKa = 5.29AIDD23 pKa = 4.8EE24 pKa = 4.39YY25 pKa = 11.05FQSQLNPEE33 pKa = 4.17QNPLSVGYY41 pKa = 10.25ISIKK45 pKa = 10.67DD46 pKa = 3.44FAYY49 pKa = 10.44DD50 pKa = 3.35VTNPLHH56 pKa = 6.03YY57 pKa = 10.3GYY59 pKa = 10.83FEE61 pKa = 4.65DD62 pKa = 5.22AEE64 pKa = 4.1EE65 pKa = 4.19RR66 pKa = 11.84ADD68 pKa = 3.59NEE70 pKa = 4.49LYY72 pKa = 11.07NEE74 pKa = 4.99DD75 pKa = 4.23EE76 pKa = 5.36HH77 pKa = 9.53DD78 pKa = 6.2DD79 pKa = 5.87DD80 pKa = 7.53DD81 pKa = 7.68DD82 pKa = 6.75DD83 pKa = 7.2DD84 pKa = 6.36NDD86 pKa = 3.65DD87 pKa = 4.08TKK89 pKa = 10.8RR90 pKa = 11.84QSIILPTNYY99 pKa = 9.35IVNQRR104 pKa = 11.84AVALYY109 pKa = 10.78DD110 pKa = 3.66FVPEE114 pKa = 4.1NDD116 pKa = 3.69SEE118 pKa = 4.64LGLEE122 pKa = 4.1EE123 pKa = 4.99GDD125 pKa = 4.02IVFISYY131 pKa = 10.25KK132 pKa = 10.27HH133 pKa = 4.96GQGWLVAEE141 pKa = 4.19NQGRR145 pKa = 11.84TKK147 pKa = 10.34TGLVPEE153 pKa = 4.53EE154 pKa = 3.88FVSFLEE160 pKa = 4.87DD161 pKa = 3.66DD162 pKa = 3.82EE163 pKa = 6.51DD164 pKa = 5.68YY165 pKa = 11.51EE166 pKa = 6.55DD167 pKa = 4.28EE168 pKa = 5.93DD169 pKa = 4.05EE170 pKa = 5.03DD171 pKa = 4.14KK172 pKa = 11.25ARR174 pKa = 11.84PFYY177 pKa = 9.85LTQFITQSINSTSTNTSYY195 pKa = 11.73NEE197 pKa = 3.75VDD199 pKa = 3.62GGEE202 pKa = 4.02DD203 pKa = 4.86DD204 pKa = 3.3EE205 pKa = 5.4WEE207 pKa = 4.76DD208 pKa = 3.9VDD210 pKa = 4.82QLEE213 pKa = 4.58SDD215 pKa = 3.43LHH217 pKa = 6.1EE218 pKa = 4.56SLKK221 pKa = 9.59ITDD224 pKa = 3.43QQ225 pKa = 3.52

Molecular weight: 26.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7TLD1|A7TLD1_VANPO RF_PROK_I domain-containing protein OS=Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) OX=436907 GN=Kpol_1020p20 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.09WRR6 pKa = 11.84KK7 pKa = 8.87KK8 pKa = 8.73RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.71RR15 pKa = 11.84KK16 pKa = 8.27RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.19VRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 10.98

Molecular weight: 3.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5314

39

5353

2697547

13

8837

503.9

56.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.681 ± 0.04

1.103 ± 0.016

6.018 ± 0.028

6.883 ± 0.048

4.255 ± 0.034

4.9 ± 0.029

1.954 ± 0.015

7.035 ± 0.046

7.254 ± 0.064

9.104 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.964 ± 0.02

6.747 ± 0.058

4.013 ± 0.028

3.632 ± 0.039

3.901 ± 0.034

9.465 ± 0.079

6.754 ± 0.188

5.786 ± 0.06

0.95 ± 0.011

3.601 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski