Gottschalkia purinilytica (Clostridium purinilyticum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Gottschalkiaceae; Gottschalkia

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3284 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L0WCL3|A0A0L0WCL3_GOTPU Peroxiredoxin OS=Gottschalkia purinilytica OX=1503 GN=CLPU_4c02650 PE=3 SV=1
MM1 pKa = 7.34TKK3 pKa = 10.54HH4 pKa = 6.31NIKK7 pKa = 10.24FCEE10 pKa = 3.93NNFQYY15 pKa = 9.82EE16 pKa = 3.74TDD18 pKa = 3.99KK19 pKa = 10.92IVKK22 pKa = 9.74ILEE25 pKa = 4.42DD26 pKa = 4.11DD27 pKa = 4.0FSDD30 pKa = 4.17VEE32 pKa = 4.59VEE34 pKa = 4.21VSSCLGYY41 pKa = 10.99CGDD44 pKa = 4.48CATSPFALIDD54 pKa = 3.82DD55 pKa = 4.24VFVNTDD61 pKa = 3.13TQEE64 pKa = 3.98EE65 pKa = 4.32LLEE68 pKa = 4.52EE69 pKa = 3.99IKK71 pKa = 10.87SYY73 pKa = 11.07IEE75 pKa = 3.78

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0W6L6|A0A0L0W6L6_GOTPU DUF3343 domain-containing protein OS=Gottschalkia purinilytica OX=1503 GN=CLPU_23c00150 PE=4 SV=1
MM1 pKa = 7.7AGGRR5 pKa = 11.84KK6 pKa = 8.74FRR8 pKa = 11.84TRR10 pKa = 11.84KK11 pKa = 9.34RR12 pKa = 11.84VCSFCADD19 pKa = 3.11KK20 pKa = 11.14GQEE23 pKa = 3.66ISYY26 pKa = 11.05KK27 pKa = 10.56DD28 pKa = 3.45VNKK31 pKa = 10.27LKK33 pKa = 10.66KK34 pKa = 10.37YY35 pKa = 6.92VTEE38 pKa = 4.26RR39 pKa = 11.84GKK41 pKa = 10.15ILPRR45 pKa = 11.84RR46 pKa = 11.84ISGNCAIHH54 pKa = 6.13QRR56 pKa = 11.84QLTTAIKK63 pKa = 10.16RR64 pKa = 11.84ARR66 pKa = 11.84QIAILPYY73 pKa = 9.77TADD76 pKa = 3.14

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3284

0

3284

939259

39

1916

286.0

32.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.961 ± 0.046

1.004 ± 0.019

5.664 ± 0.033

7.631 ± 0.055

4.19 ± 0.031

6.361 ± 0.044

1.413 ± 0.016

10.48 ± 0.053

9.366 ± 0.055

9.145 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.021

6.143 ± 0.042

2.816 ± 0.027

2.391 ± 0.02

3.507 ± 0.024

6.478 ± 0.035

4.978 ± 0.03

6.364 ± 0.036

0.678 ± 0.015

3.947 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski