Flammeovirga sp. SJP92

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Flammeovirgaceae; Flammeovirga; unclassified Flammeovirga

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A150ARR8|A0A150ARR8_9BACT Uncharacterized protein OS=Flammeovirga sp. SJP92 OX=1775430 GN=AVL50_06405 PE=4 SV=1
MM1 pKa = 7.5LFATAVEE8 pKa = 4.24AQTTFRR14 pKa = 11.84PNKK17 pKa = 10.14LGTLNISSTGDD28 pKa = 2.82WQTASNWDD36 pKa = 3.64CGGCGSFPGSGDD48 pKa = 2.75IVLFGNDD55 pKa = 3.02NIYY58 pKa = 10.32WGDD61 pKa = 3.14AAIIIEE67 pKa = 4.26NVTLSGITFTASAEE81 pKa = 4.27GAALTIGSGATVNATSINEE100 pKa = 3.82IGKK103 pKa = 8.37NTISINSGGTLTINNSLTLDD123 pKa = 3.65NDD125 pKa = 3.53GTAFNVDD132 pKa = 2.74GDD134 pKa = 4.0VTINGDD140 pKa = 4.22LILSGGAAQTINSSGTLTVNGDD162 pKa = 3.45LSTEE166 pKa = 4.2GGSLNVTGGNVGITGDD182 pKa = 3.3WTIPSDD188 pKa = 4.13QANGNNGISISSGASVAIGGDD209 pKa = 3.11IAFLGNNSIPNPDD222 pKa = 3.22SQINIDD228 pKa = 4.16GSFTFNGSCTNNDD241 pKa = 3.82GGNDD245 pKa = 3.49LCQTFQDD252 pKa = 4.33INDD255 pKa = 3.96DD256 pKa = 3.87SLPVVLISLFGIQTEE271 pKa = 4.25EE272 pKa = 4.1GFLVKK277 pKa = 8.59WTTASEE283 pKa = 4.25QNNSHH288 pKa = 7.42FIIEE292 pKa = 4.71GSNSTDD298 pKa = 2.57NWKK301 pKa = 10.54EE302 pKa = 3.46IAEE305 pKa = 4.08VDD307 pKa = 3.79GQGNTNTSTDD317 pKa = 3.57YY318 pKa = 11.34EE319 pKa = 4.22IVLKK323 pKa = 10.87GEE325 pKa = 3.76QYY327 pKa = 10.78SYY329 pKa = 11.65YY330 pKa = 10.85RR331 pKa = 11.84LIQYY335 pKa = 10.33DD336 pKa = 3.57FDD338 pKa = 4.74GSFEE342 pKa = 3.99IFGILNQDD350 pKa = 2.73ISNTDD355 pKa = 3.05RR356 pKa = 11.84SVHH359 pKa = 5.7IFPNEE364 pKa = 3.69VNQGQFINLLFANGNADD381 pKa = 4.75LSITLQLYY389 pKa = 10.33DD390 pKa = 5.06LIGNVLDD397 pKa = 4.0YY398 pKa = 11.38QSVPNYY404 pKa = 10.3NSSLYY409 pKa = 9.72QYY411 pKa = 10.43KK412 pKa = 9.75VPNTAKK418 pKa = 10.77DD419 pKa = 3.1GGYY422 pKa = 10.55YY423 pKa = 9.76ILSVLIGNKK432 pKa = 9.51RR433 pKa = 11.84FHH435 pKa = 6.5QKK437 pKa = 10.29VLIKK441 pKa = 10.73

Molecular weight: 46.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A150ADA6|A0A150ADA6_9BACT Uncharacterized protein OS=Flammeovirga sp. SJP92 OX=1775430 GN=AVL50_25135 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 8.89RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 10.04HH16 pKa = 4.44GFRR19 pKa = 11.84QRR21 pKa = 11.84MLTANGRR28 pKa = 11.84NVLAARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.6KK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.81SLTVSDD46 pKa = 4.03SRR48 pKa = 11.84KK49 pKa = 10.05HH50 pKa = 5.27KK51 pKa = 10.79AA52 pKa = 3.07

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6269

0

6269

2376931

52

6570

379.2

42.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.755 ± 0.034

0.718 ± 0.011

5.826 ± 0.025

7.165 ± 0.029

5.122 ± 0.02

6.501 ± 0.031

1.908 ± 0.017

7.491 ± 0.031

7.514 ± 0.046

8.987 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.272 ± 0.015

5.977 ± 0.029

3.332 ± 0.019

3.65 ± 0.018

3.35 ± 0.018

6.8 ± 0.03

5.735 ± 0.041

6.262 ± 0.026

1.213 ± 0.011

4.422 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski