Hubei sobemo-like virus 37

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEI4|A0A1L3KEI4_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 37 OX=1923224 PE=4 SV=1
MM1 pKa = 8.21DD2 pKa = 4.34STPGFPFCRR11 pKa = 11.84HH12 pKa = 5.15YY13 pKa = 9.88KK14 pKa = 9.62TNAEE18 pKa = 3.89LLGFNGLNVDD28 pKa = 3.48HH29 pKa = 7.51SKK31 pKa = 8.37TQIVVSSFLKK41 pKa = 9.54WLEE44 pKa = 4.3DD45 pKa = 3.24PVEE48 pKa = 3.99YY49 pKa = 10.42NFRR52 pKa = 11.84MFIKK56 pKa = 10.41DD57 pKa = 3.67EE58 pKa = 3.84PHH60 pKa = 6.51KK61 pKa = 10.21PSKK64 pKa = 10.29VDD66 pKa = 3.13EE67 pKa = 4.55GRR69 pKa = 11.84WRR71 pKa = 11.84LIFSAPLFYY80 pKa = 10.69QILEE84 pKa = 4.22HH85 pKa = 7.18LLLDD89 pKa = 4.69PLDD92 pKa = 4.21EE93 pKa = 5.18LEE95 pKa = 6.28KK96 pKa = 11.01DD97 pKa = 4.19NQWSLPTKK105 pKa = 10.43LGWHH109 pKa = 6.44PFWGGAQMCRR119 pKa = 11.84STFDD123 pKa = 4.14KK124 pKa = 10.24PCSMDD129 pKa = 3.39KK130 pKa = 10.94KK131 pKa = 10.24CWDD134 pKa = 3.29WTLTHH139 pKa = 6.46YY140 pKa = 10.74LVEE143 pKa = 5.67LDD145 pKa = 3.37TQLRR149 pKa = 11.84KK150 pKa = 9.93RR151 pKa = 11.84LVIAPDD157 pKa = 2.57IWYY160 pKa = 9.54DD161 pKa = 3.96LLDD164 pKa = 4.41KK165 pKa = 9.69LTSMSFYY172 pKa = 9.56TASFQFSSGHH182 pKa = 5.3VFRR185 pKa = 11.84QTKK188 pKa = 8.97EE189 pKa = 4.15GIMKK193 pKa = 10.31SGLVRR198 pKa = 11.84TLSTNSHH205 pKa = 5.47CQYY208 pKa = 10.37FIHH211 pKa = 7.27LLACNKK217 pKa = 9.85CGHH220 pKa = 6.01NHH222 pKa = 4.87VCWIIGDD229 pKa = 4.05DD230 pKa = 4.26VIVSDD235 pKa = 4.16PCSEE239 pKa = 3.91YY240 pKa = 11.19LEE242 pKa = 4.5YY243 pKa = 10.35TSHH246 pKa = 6.31YY247 pKa = 10.12CLLKK251 pKa = 10.93DD252 pKa = 4.37VDD254 pKa = 4.08WNYY257 pKa = 10.78HH258 pKa = 4.71FAGFDD263 pKa = 3.5LLKK266 pKa = 10.67NVPLYY271 pKa = 9.6WSKK274 pKa = 10.69HH275 pKa = 4.02VSRR278 pKa = 11.84LLYY281 pKa = 10.82SSDD284 pKa = 3.23EE285 pKa = 4.43VIPEE289 pKa = 3.84ILDD292 pKa = 3.42NYY294 pKa = 8.75QRR296 pKa = 11.84LYY298 pKa = 11.23VYY300 pKa = 10.11DD301 pKa = 3.4QSKK304 pKa = 10.74FNFFQRR310 pKa = 11.84LLAEE314 pKa = 4.27YY315 pKa = 9.87DD316 pKa = 3.47LSKK319 pKa = 10.83LRR321 pKa = 11.84SRR323 pKa = 11.84DD324 pKa = 3.42YY325 pKa = 10.95LLRR328 pKa = 11.84WSVQKK333 pKa = 10.56PSNFKK338 pKa = 10.99FMFKK342 pKa = 9.39TT343 pKa = 3.48

Molecular weight: 40.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEI3|A0A1L3KEI3_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 37 OX=1923224 PE=4 SV=1
MM1 pKa = 7.18VLNVMLVLGALEE13 pKa = 4.52TILLQHH19 pKa = 6.42HH20 pKa = 6.43MLYY23 pKa = 9.77PVLMVGYY30 pKa = 8.38MFQQLIILLKK40 pKa = 9.36YY41 pKa = 10.08VIIGLYY47 pKa = 9.57IIMMKK52 pKa = 10.16FLSWKK57 pKa = 9.59FLLVFLLLYY66 pKa = 10.76KK67 pKa = 10.39FDD69 pKa = 4.39LLKK72 pKa = 10.73LVPPMIRR79 pKa = 11.84WFVLPDD85 pKa = 3.48VKK87 pKa = 10.74LSNPPLPEE95 pKa = 3.43YY96 pKa = 10.47SYY98 pKa = 10.62PQMYY102 pKa = 10.16LGFIGIVEE110 pKa = 4.02QLSRR114 pKa = 11.84GILEE118 pKa = 4.0LPIWSVIIQLLPCIFLVAILVICVYY143 pKa = 11.16LLVTYY148 pKa = 9.86KK149 pKa = 9.96WLSSSSLSLLDD160 pKa = 4.49LKK162 pKa = 10.48TNLKK166 pKa = 10.98SMVLYY171 pKa = 9.41WLLRR175 pKa = 11.84ILRR178 pKa = 11.84CLVMLVMNLFFRR190 pKa = 11.84GFVLIYY196 pKa = 9.76QGSRR200 pKa = 11.84LLFLKK205 pKa = 10.69AKK207 pKa = 10.43AKK209 pKa = 9.91IKK211 pKa = 10.85RR212 pKa = 11.84EE213 pKa = 4.05DD214 pKa = 2.65VWYY217 pKa = 11.05RR218 pKa = 11.84MIGMKK223 pKa = 10.16KK224 pKa = 9.83IYY226 pKa = 10.28LKK228 pKa = 10.58LKK230 pKa = 10.02KK231 pKa = 10.14YY232 pKa = 9.82VLAVLVLILKK242 pKa = 8.19ITSSNLVNIIILLMMLHH259 pKa = 6.42LVVSKK264 pKa = 10.48SWLVINLFQFILVMLGLMVEE284 pKa = 4.63VSAFLMKK291 pKa = 10.42MMMNLLFF298 pKa = 4.6

Molecular weight: 34.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

922

281

343

307.3

35.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.82 ± 0.591

1.952 ± 0.488

5.206 ± 1.719

4.23 ± 1.107

5.315 ± 0.745

4.664 ± 0.993

2.386 ± 0.802

6.074 ± 1.63

6.616 ± 0.368

14.751 ± 4.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.796 ± 1.584

3.688 ± 0.492

4.013 ± 0.291

3.362 ± 0.319

5.098 ± 1.479

7.484 ± 0.786

3.579 ± 1.004

7.592 ± 1.33

2.278 ± 0.661

5.098 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski