Mariprofundus sp. EBB-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Zetaproteobacteria; Mariprofundales; Mariprofundaceae; Mariprofundus; unclassified Mariprofundus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2630 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A498DK54|A0A498DK54_9PROT Thioredoxin OS=Mariprofundus sp. EBB-1 OX=2650971 GN=D8Y20_06350 PE=4 SV=1
MM1 pKa = 7.36QIDD4 pKa = 3.47NHH6 pKa = 6.22KK7 pKa = 10.43VVSFHH12 pKa = 6.0YY13 pKa = 9.82TLTNDD18 pKa = 2.88SGTVIDD24 pKa = 4.5ASEE27 pKa = 4.24GRR29 pKa = 11.84DD30 pKa = 3.35PLVYY34 pKa = 10.51LHH36 pKa = 6.89GEE38 pKa = 4.01EE39 pKa = 4.54NIIPGLEE46 pKa = 3.7LALEE50 pKa = 4.69GKK52 pKa = 10.21AVGDD56 pKa = 3.76KK57 pKa = 11.18LKK59 pKa = 11.08VSLDD63 pKa = 3.19AADD66 pKa = 4.46AYY68 pKa = 11.06GEE70 pKa = 4.13FDD72 pKa = 3.43PAMVEE77 pKa = 4.23IVSSDD82 pKa = 3.09MFDD85 pKa = 3.59DD86 pKa = 4.12VEE88 pKa = 4.4SVEE91 pKa = 4.51VGMEE95 pKa = 4.2FQAEE99 pKa = 4.21LPDD102 pKa = 4.26GEE104 pKa = 4.6GLQMVRR110 pKa = 11.84ITDD113 pKa = 3.37VDD115 pKa = 3.68GDD117 pKa = 3.94NVTVDD122 pKa = 3.91GNHH125 pKa = 7.05PLAGQQLNFDD135 pKa = 3.97VAIVDD140 pKa = 3.86IRR142 pKa = 11.84DD143 pKa = 3.43ATADD147 pKa = 3.76EE148 pKa = 4.91LEE150 pKa = 4.78HH151 pKa = 6.69GHH153 pKa = 4.96VHH155 pKa = 7.13GDD157 pKa = 3.65EE158 pKa = 4.37EE159 pKa = 5.28CCGEE163 pKa = 4.39GGCGSDD169 pKa = 4.36DD170 pKa = 3.68CCSS173 pKa = 3.11

Molecular weight: 18.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A498DKK1|A0A498DKK1_9PROT ATP-dependent dethiobiotin synthetase BioD OS=Mariprofundus sp. EBB-1 OX=2650971 GN=bioD PE=3 SV=1
MM1 pKa = 7.5SFTYY5 pKa = 9.9KK6 pKa = 10.15PSRR9 pKa = 11.84IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84SGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.5GRR39 pKa = 11.84VRR41 pKa = 11.84LSTT44 pKa = 3.52

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2630

0

2630

848503

32

4336

322.6

35.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.608 ± 0.061

1.113 ± 0.018

5.81 ± 0.037

5.903 ± 0.065

3.737 ± 0.028

7.142 ± 0.049

2.74 ± 0.032

6.368 ± 0.039

4.839 ± 0.044

10.278 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.097 ± 0.028

3.708 ± 0.04

3.966 ± 0.029

4.175 ± 0.038

5.195 ± 0.048

6.521 ± 0.042

5.07 ± 0.059

6.84 ± 0.049

1.285 ± 0.022

2.605 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski