Panicum miliaceum (Proso millet) (Broomcorn millet)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Panicodae; Paniceae; Panicinae; Panicum

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55732 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3L6Q1F1|A0A3L6Q1F1_PANMI Zingipain-2-like OS=Panicum miliaceum OX=4540 GN=C2845_PM17G08540 PE=3 SV=1
MM1 pKa = 7.39IADD4 pKa = 3.83SGTIFTILVEE14 pKa = 4.16SAFRR18 pKa = 11.84VIVDD22 pKa = 3.59SVAGVLGQPVVNASSLDD39 pKa = 3.99SPCFPAPPGEE49 pKa = 4.22WPLPDD54 pKa = 3.74MPDD57 pKa = 3.48MVLHH61 pKa = 6.44FADD64 pKa = 4.65GADD67 pKa = 3.24MRR69 pKa = 11.84LPRR72 pKa = 11.84DD73 pKa = 3.05NYY75 pKa = 9.67MSFNQEE81 pKa = 3.14EE82 pKa = 4.35AAFCLTIVGTTYY94 pKa = 10.79SVSVLGNFQQQNIQMLYY111 pKa = 10.45DD112 pKa = 3.29ITVGQLSFVPTDD124 pKa = 3.48CSKK127 pKa = 11.31LL128 pKa = 3.65

Molecular weight: 13.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3L6SEQ0|A0A3L6SEQ0_PANMI Fe2OG dioxygenase domain-containing protein OS=Panicum miliaceum OX=4540 GN=C2845_PM02G21880 PE=3 SV=1
MM1 pKa = 7.65AWRR4 pKa = 11.84GASSRR9 pKa = 11.84SLLAAVRR16 pKa = 11.84GRR18 pKa = 11.84ASSSTAAAASRR29 pKa = 11.84VRR31 pKa = 11.84AAAPIPAAPRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84VPAFAFATASVV54 pKa = 3.4

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55732

0

55732

21762937

29

5288

390.5

42.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.848 ± 0.016

1.886 ± 0.005

5.398 ± 0.008

6.022 ± 0.011

3.604 ± 0.006

7.505 ± 0.01

2.428 ± 0.005

4.165 ± 0.008

4.806 ± 0.01

9.448 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.358 ± 0.004

3.345 ± 0.008

5.806 ± 0.01

3.429 ± 0.007

6.551 ± 0.009

8.128 ± 0.011

4.681 ± 0.006

6.769 ± 0.008

1.31 ± 0.004

2.513 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski