Clostridium sp. CAG:7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2911 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5J0U2|R5J0U2_9CLOT BTAD domain-containing protein OS=Clostridium sp. CAG:7 OX=1262832 GN=BN757_02283 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 9.9KK3 pKa = 9.22WKK5 pKa = 10.23SKK7 pKa = 10.09LFPVITLAVCLGLAACGGGAEE28 pKa = 4.73SEE30 pKa = 4.48GSSDD34 pKa = 3.65DD35 pKa = 3.73QEE37 pKa = 5.94DD38 pKa = 4.05YY39 pKa = 11.75EE40 pKa = 4.38EE41 pKa = 4.83TEE43 pKa = 3.89NSAPEE48 pKa = 4.21SFEE51 pKa = 5.12GMVKK55 pKa = 9.61DD56 pKa = 3.72TDD58 pKa = 3.17ITAVYY63 pKa = 10.72DD64 pKa = 4.0MYY66 pKa = 10.08WGTWIGEE73 pKa = 4.18DD74 pKa = 3.91DD75 pKa = 3.96SEE77 pKa = 6.24LIVGLNGSGEE87 pKa = 4.58DD88 pKa = 3.16RR89 pKa = 11.84FEE91 pKa = 4.72WYY93 pKa = 10.08DD94 pKa = 3.5EE95 pKa = 4.66DD96 pKa = 5.1DD97 pKa = 3.82NLMASGYY104 pKa = 8.58IQLVPEE110 pKa = 4.1YY111 pKa = 10.01EE112 pKa = 3.51ADD114 pKa = 3.49YY115 pKa = 10.23FYY117 pKa = 11.44NEE119 pKa = 3.87HH120 pKa = 7.35DD121 pKa = 3.62GWAHH125 pKa = 5.54HH126 pKa = 5.72VWRR129 pKa = 11.84DD130 pKa = 3.01EE131 pKa = 4.64DD132 pKa = 3.93GALHH136 pKa = 6.7IDD138 pKa = 3.55SFGVFTKK145 pKa = 10.56KK146 pKa = 10.29PLGRR150 pKa = 11.84DD151 pKa = 3.37VSEE154 pKa = 4.41SDD156 pKa = 3.71CEE158 pKa = 3.99EE159 pKa = 4.06LAGVWFLDD167 pKa = 3.83GEE169 pKa = 4.25ADD171 pKa = 3.28AASVIEE177 pKa = 3.99IEE179 pKa = 5.47KK180 pKa = 10.73DD181 pKa = 3.81GSWSLSEE188 pKa = 5.27RR189 pKa = 11.84PDD191 pKa = 3.45GDD193 pKa = 3.55GDD195 pKa = 3.92LAEE198 pKa = 4.42VDD200 pKa = 3.85SGILQVNEE208 pKa = 4.65RR209 pKa = 11.84GDD211 pKa = 3.6DD212 pKa = 3.33QYY214 pKa = 11.88FAVSEE219 pKa = 4.11QFDD222 pKa = 3.99DD223 pKa = 3.63VFYY226 pKa = 10.09PITLAEE232 pKa = 4.59PGAMYY237 pKa = 9.82WGYY240 pKa = 8.93TNEE243 pKa = 3.89YY244 pKa = 8.96YY245 pKa = 10.39EE246 pKa = 4.63RR247 pKa = 11.84SEE249 pKa = 3.94

Molecular weight: 27.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5IXC2|R5IXC2_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:7 OX=1262832 GN=BN757_01673 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.44VHH16 pKa = 5.59GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.5TAGGRR28 pKa = 11.84KK29 pKa = 9.01VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.3GRR39 pKa = 11.84AKK41 pKa = 9.64LTAA44 pKa = 4.21

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2911

0

2911

951847

29

3741

327.0

36.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.031 ± 0.052

1.514 ± 0.018

5.55 ± 0.037

7.503 ± 0.055

4.044 ± 0.033

7.556 ± 0.042

1.769 ± 0.019

6.911 ± 0.035

6.744 ± 0.036

9.091 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.278 ± 0.023

3.994 ± 0.027

3.542 ± 0.029

3.241 ± 0.029

4.245 ± 0.032

5.672 ± 0.035

5.415 ± 0.033

6.996 ± 0.041

0.977 ± 0.015

3.922 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski