Anaerosporomusa subterranea

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; Anaerosporomusa

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3585 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A154BSH0|A0A154BSH0_ANASB GDSL family lipase OS=Anaerosporomusa subterranea OX=1794912 GN=AXX12_02080 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.53LGDD5 pKa = 4.1LSAKK9 pKa = 10.29YY10 pKa = 9.86EE11 pKa = 4.59SNGDD15 pKa = 3.48PGAISSGEE23 pKa = 3.75GDD25 pKa = 3.43AGGVSYY31 pKa = 10.7GAYY34 pKa = 9.8QFAANAGVPGQFVAWLKK51 pKa = 10.62QIGYY55 pKa = 10.01LYY57 pKa = 10.87ADD59 pKa = 4.19EE60 pKa = 4.6LAEE63 pKa = 4.62AGVPGCDD70 pKa = 3.27EE71 pKa = 5.88FSDD74 pKa = 3.22AWLRR78 pKa = 11.84AAARR82 pKa = 11.84DD83 pKa = 3.57PDD85 pKa = 3.9GFLAAQHH92 pKa = 5.58EE93 pKa = 5.0FVRR96 pKa = 11.84QSYY99 pKa = 8.87YY100 pKa = 10.88EE101 pKa = 3.97PARR104 pKa = 11.84EE105 pKa = 3.65QALAAGINIDD115 pKa = 3.56GCSFALQNVVWSAAVQYY132 pKa = 9.93GAYY135 pKa = 9.03YY136 pKa = 10.41VKK138 pKa = 10.68EE139 pKa = 4.17LFEE142 pKa = 4.99DD143 pKa = 3.89AATQLGVTSAADD155 pKa = 3.59ADD157 pKa = 4.13DD158 pKa = 4.12AALIQAIYY166 pKa = 10.23DD167 pKa = 3.53VRR169 pKa = 11.84ASDD172 pKa = 3.37EE173 pKa = 4.22WTTGSPEE180 pKa = 4.08LRR182 pKa = 11.84PGLIARR188 pKa = 11.84FEE190 pKa = 4.28AEE192 pKa = 4.65CRR194 pKa = 11.84DD195 pKa = 3.55ALAALDD201 pKa = 4.12SEE203 pKa = 4.82

Molecular weight:
21.53 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A154BP56|A0A154BP56_ANASB 4-hydroxy-tetrahydrodipicolinate synthase OS=Anaerosporomusa subterranea OX=1794912 GN=dapA PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.52RR3 pKa = 11.84TFQPNVSWRR12 pKa = 11.84KK13 pKa = 6.99KK14 pKa = 6.42THH16 pKa = 5.13GFRR19 pKa = 11.84EE20 pKa = 4.31RR21 pKa = 11.84MSSLGGRR28 pKa = 11.84QVLKK32 pKa = 10.45RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.19GRR39 pKa = 11.84KK40 pKa = 8.79KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight:
5.26 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3585

0

3585

1103724

37

1441

307.9

33.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.548 ± 0.045

1.162 ± 0.017

4.949 ± 0.031

5.992 ± 0.035

3.895 ± 0.028

7.666 ± 0.037

1.875 ± 0.017

7.248 ± 0.035

5.298 ± 0.032

10.162 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.739 ± 0.021

3.702 ± 0.024

4.07 ± 0.024

3.851 ± 0.028

4.937 ± 0.031

5.817 ± 0.028

5.454 ± 0.026

7.698 ± 0.032

0.942 ± 0.016

2.994 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski