Moraxella phage Mcat14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R6PIB4|A0A0R6PIB4_9CAUD Coat protein OS=Moraxella phage Mcat14 OX=1647529 PE=4 SV=1
MM1 pKa = 8.07LGEE4 pKa = 4.3LVNDD8 pKa = 3.66RR9 pKa = 11.84VYY11 pKa = 11.12PLFVPEE17 pKa = 4.74TADD20 pKa = 3.26STPPFMVYY28 pKa = 9.4TLVSNVPDD36 pKa = 3.65NTLDD40 pKa = 3.51GATGHH45 pKa = 6.12EE46 pKa = 4.34WVGVQIDD53 pKa = 4.68IYY55 pKa = 11.07AADD58 pKa = 4.0YY59 pKa = 11.55DD60 pKa = 4.13EE61 pKa = 5.35TIALAQEE68 pKa = 3.92AVKK71 pKa = 10.44RR72 pKa = 11.84LNTIKK77 pKa = 10.09PSEE80 pKa = 4.32YY81 pKa = 9.31GGVVYY86 pKa = 10.9VHH88 pKa = 7.72DD89 pKa = 5.59DD90 pKa = 3.19GLYY93 pKa = 9.37RR94 pKa = 11.84AIIEE98 pKa = 4.2YY99 pKa = 9.78EE100 pKa = 4.07FWQTIII106 pKa = 4.15

Molecular weight: 11.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R6PI74|A0A0R6PI74_9CAUD N(6)-L-threonylcarbamoyladenine synthase OS=Moraxella phage Mcat14 OX=1647529 PE=3 SV=1
MM1 pKa = 7.2MFYY4 pKa = 10.59VWGWSHH10 pKa = 6.09EE11 pKa = 4.39KK12 pKa = 10.24IAVRR16 pKa = 11.84YY17 pKa = 9.1LSAIGYY23 pKa = 8.4RR24 pKa = 11.84GKK26 pKa = 10.63SRR28 pKa = 11.84ASRR31 pKa = 11.84HH32 pKa = 5.37DD33 pKa = 3.4VRR35 pKa = 11.84SALIKK40 pKa = 10.73AEE42 pKa = 4.06SFLAGLLHH50 pKa = 6.76KK51 pKa = 10.43LYY53 pKa = 11.03SFTT56 pKa = 4.73

Molecular weight: 6.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

5223

37

1080

217.6

24.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.879 ± 0.903

0.785 ± 0.266

6.337 ± 0.552

6.337 ± 0.401

3.408 ± 0.163

7.141 ± 0.427

1.685 ± 0.361

6.452 ± 0.562

7.601 ± 0.697

9.324 ± 0.42

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.968 ± 0.317

4.691 ± 0.341

3.121 ± 0.403

4.27 ± 0.332

4.27 ± 0.629

5.954 ± 0.389

5.437 ± 0.303

5.993 ± 0.351

1.053 ± 0.226

3.293 ± 0.349
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski