Faecalibacterium phage FP_Brigit

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Brigitvirus; Faecalibacterium virus Brigit

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9V3M3|A0A2K9V3M3_9CAUD Holin OS=Faecalibacterium phage FP_Brigit OX=2070181 PE=4 SV=1
MM1 pKa = 7.45TDD3 pKa = 2.97EE4 pKa = 5.07KK5 pKa = 10.85KK6 pKa = 10.88FEE8 pKa = 3.88VHH10 pKa = 7.07AEE12 pKa = 3.61ITVRR16 pKa = 11.84LTQQDD21 pKa = 2.99VDD23 pKa = 4.8DD24 pKa = 4.91IMVSALEE31 pKa = 4.27GGICYY36 pKa = 9.98WSDD39 pKa = 3.24CAEE42 pKa = 4.06VVGCYY47 pKa = 9.84LSEE50 pKa = 4.17YY51 pKa = 10.88ASGQISRR58 pKa = 11.84GGKK61 pKa = 9.77LKK63 pKa = 10.39IHH65 pKa = 6.23VDD67 pKa = 3.71EE68 pKa = 5.62PFDD71 pKa = 3.56EE72 pKa = 6.13ANTEE76 pKa = 3.88WYY78 pKa = 9.37EE79 pKa = 4.24LDD81 pKa = 3.46MEE83 pKa = 4.76KK84 pKa = 10.21FAQGFRR90 pKa = 11.84LWLEE94 pKa = 4.13NGGDD98 pKa = 3.48RR99 pKa = 11.84YY100 pKa = 10.91GAVSNGEE107 pKa = 3.93VDD109 pKa = 3.05TCEE112 pKa = 4.55IDD114 pKa = 4.98GEE116 pKa = 4.32MADD119 pKa = 6.05LIIQYY124 pKa = 11.01ALFGEE129 pKa = 4.85VVYY132 pKa = 10.83GG133 pKa = 3.74

Molecular weight: 14.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9V3P4|A0A2K9V3P4_9CAUD Tail protein OS=Faecalibacterium phage FP_Brigit OX=2070181 PE=4 SV=1
MM1 pKa = 7.48EE2 pKa = 5.48NKK4 pKa = 10.27NMTAARR10 pKa = 11.84EE11 pKa = 4.25WEE13 pKa = 3.88NDD15 pKa = 3.4PNCFLRR21 pKa = 11.84MLNSPAQQRR30 pKa = 11.84SRR32 pKa = 11.84AARR35 pKa = 11.84RR36 pKa = 11.84QKK38 pKa = 10.32DD39 pKa = 3.25AYY41 pKa = 10.24RR42 pKa = 11.84EE43 pKa = 3.95RR44 pKa = 11.84FNNVLNAVAIGAAAFAVTLLVICFVLL70 pKa = 4.32

Molecular weight: 7.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

19007

37

828

202.2

22.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.228 ± 0.349

1.563 ± 0.127

6.871 ± 0.238

7.192 ± 0.29

3.194 ± 0.152

7.045 ± 0.246

1.405 ± 0.088

5.314 ± 0.203

6.513 ± 0.207

7.729 ± 0.236

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.138

3.941 ± 0.181

3.778 ± 0.207

3.483 ± 0.149

5.177 ± 0.289

6.224 ± 0.286

6.271 ± 0.325

7.176 ± 0.274

1.352 ± 0.117

3.688 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski