Amino acid dipepetide frequency for Thioclava sp. SK-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.667AlaAla: 15.667 ± 0.17	1.155AlaCys: 1.155 ± 0.04	7.007AlaAsp: 7.007 ± 0.102	6.642AlaGlu: 6.642 ± 0.114	3.966AlaPhe: 3.966 ± 0.064	10.115AlaGly: 10.115 ± 0.184	2.381AlaHis: 2.381 ± 0.052	6.142AlaIle: 6.142 ± 0.077	4.07AlaLys: 4.07 ± 0.069	13.587AlaLeu: 13.587 ± 0.134	3.874AlaMet: 3.874 ± 0.062	2.796AlaAsn: 2.796 ± 0.053	5.859AlaPro: 5.859 ± 0.081	6.792AlaGln: 6.792 ± 0.116	7.962AlaArg: 7.962 ± 0.102	5.635AlaSer: 5.635 ± 0.089	6.191AlaThr: 6.191 ± 0.105	8.214AlaVal: 8.214 ± 0.106	1.406AlaTrp: 1.406 ± 0.038	2.432AlaTyr: 2.432 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.247CysAla: 1.247 ± 0.039	0.116CysCys: 0.116 ± 0.01	0.702CysAsp: 0.702 ± 0.027	0.441CysGlu: 0.441 ± 0.018	0.338CysPhe: 0.338 ± 0.017	0.916CysGly: 0.916 ± 0.032	0.282CysHis: 0.282 ± 0.016	0.439CysIle: 0.439 ± 0.018	0.211CysLys: 0.211 ± 0.011	0.916CysLeu: 0.916 ± 0.033	0.165CysMet: 0.165 ± 0.011	0.198CysAsn: 0.198 ± 0.013	0.525CysPro: 0.525 ± 0.02	0.29CysGln: 0.29 ± 0.015	0.539CysArg: 0.539 ± 0.024	0.463CysSer: 0.463 ± 0.021	0.484CysThr: 0.484 ± 0.021	0.685CysVal: 0.685 ± 0.027	0.118CysTrp: 0.118 ± 0.012	0.203CysTyr: 0.203 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.357AspAla: 7.357 ± 0.086	0.525AspCys: 0.525 ± 0.023	3.609AspAsp: 3.609 ± 0.07	3.173AspGlu: 3.173 ± 0.07	2.254AspPhe: 2.254 ± 0.051	5.706AspGly: 5.706 ± 0.119	1.498AspHis: 1.498 ± 0.043	3.416AspIle: 3.416 ± 0.076	1.67AspLys: 1.67 ± 0.049	6.949AspLeu: 6.949 ± 0.12	1.847AspMet: 1.847 ± 0.038	1.32AspAsn: 1.32 ± 0.037	3.681AspPro: 3.681 ± 0.066	2.257AspGln: 2.257 ± 0.045	4.038AspArg: 4.038 ± 0.056	2.372AspSer: 2.372 ± 0.05	3.294AspThr: 3.294 ± 0.088	4.244AspVal: 4.244 ± 0.085	1.105AspTrp: 1.105 ± 0.033	1.566AspTyr: 1.566 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	6.708GluAla: 6.708 ± 0.114	0.35GluCys: 0.35 ± 0.017	3.205GluAsp: 3.205 ± 0.065	2.909GluGlu: 2.909 ± 0.069	1.902GluPhe: 1.902 ± 0.044	3.96GluGly: 3.96 ± 0.078	1.101GluHis: 1.101 ± 0.033	3.258GluIle: 3.258 ± 0.061	1.941GluLys: 1.941 ± 0.053	5.022GluLeu: 5.022 ± 0.077	1.638GluMet: 1.638 ± 0.039	1.677GluAsn: 1.677 ± 0.038	2.253GluPro: 2.253 ± 0.061	2.007GluGln: 2.007 ± 0.048	3.807GluArg: 3.807 ± 0.077	1.938GluSer: 1.938 ± 0.042	3.438GluThr: 3.438 ± 0.055	3.6GluVal: 3.6 ± 0.067	0.683GluTrp: 0.683 ± 0.03	1.059GluTyr: 1.059 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.441PheAla: 4.441 ± 0.064	0.432PheCys: 0.432 ± 0.021	2.955PheAsp: 2.955 ± 0.052	1.944PheGlu: 1.944 ± 0.043	1.374PhePhe: 1.374 ± 0.041	3.625PheGly: 3.625 ± 0.063	0.753PheHis: 0.753 ± 0.028	1.674PheIle: 1.674 ± 0.039	0.981PheLys: 0.981 ± 0.029	3.302PheLeu: 3.302 ± 0.064	0.87PheMet: 0.87 ± 0.03	1.114PheAsn: 1.114 ± 0.03	1.486PhePro: 1.486 ± 0.042	1.067PheGln: 1.067 ± 0.032	1.957PheArg: 1.957 ± 0.049	2.2PheSer: 2.2 ± 0.043	2.167PheThr: 2.167 ± 0.051	2.599PheVal: 2.599 ± 0.049	0.557PheTrp: 0.557 ± 0.026	0.915PheTyr: 0.915 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	9.931GlyAla: 9.931 ± 0.226	0.87GlyCys: 0.87 ± 0.032	4.632GlyAsp: 4.632 ± 0.138	4.084GlyGlu: 4.084 ± 0.069	3.588GlyPhe: 3.588 ± 0.06	6.914GlyGly: 6.914 ± 0.131	1.952GlyHis: 1.952 ± 0.04	4.444GlyIle: 4.444 ± 0.068	2.981GlyLys: 2.981 ± 0.057	9.164GlyLeu: 9.164 ± 0.097	2.605GlyMet: 2.605 ± 0.059	2.166GlyAsn: 2.166 ± 0.069	3.515GlyPro: 3.515 ± 0.063	3.567GlyGln: 3.567 ± 0.066	5.266GlyArg: 5.266 ± 0.071	4.22GlySer: 4.22 ± 0.11	4.85GlyThr: 4.85 ± 0.147	6.359GlyVal: 6.359 ± 0.091	1.448GlyTrp: 1.448 ± 0.04	2.274GlyTyr: 2.274 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.342HisAla: 2.342 ± 0.052	0.256HisCys: 0.256 ± 0.014	1.448HisAsp: 1.448 ± 0.036	1.093HisGlu: 1.093 ± 0.034	0.8HisPhe: 0.8 ± 0.027	1.906HisGly: 1.906 ± 0.043	0.622HisHis: 0.622 ± 0.024	1.123HisIle: 1.123 ± 0.036	0.556HisLys: 0.556 ± 0.025	2.188HisLeu: 2.188 ± 0.052	0.646HisMet: 0.646 ± 0.028	0.503HisAsn: 0.503 ± 0.02	1.348HisPro: 1.348 ± 0.039	0.699HisGln: 0.699 ± 0.025	1.376HisArg: 1.376 ± 0.04	1.09HisSer: 1.09 ± 0.03	0.869HisThr: 0.869 ± 0.03	1.512HisVal: 1.512 ± 0.035	0.346HisTrp: 0.346 ± 0.018	0.543HisTyr: 0.543 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.307IleAla: 7.307 ± 0.081	0.763IleCys: 0.763 ± 0.03	3.839IleAsp: 3.839 ± 0.059	3.29IleGlu: 3.29 ± 0.061	1.891IlePhe: 1.891 ± 0.051	5.166IleGly: 5.166 ± 0.078	1.022IleHis: 1.022 ± 0.026	2.41IleIle: 2.41 ± 0.048	1.408IleLys: 1.408 ± 0.035	4.897IleLeu: 4.897 ± 0.087	1.169IleMet: 1.169 ± 0.041	1.464IleAsn: 1.464 ± 0.036	2.337IlePro: 2.337 ± 0.053	1.33IleGln: 1.33 ± 0.033	3.165IleArg: 3.165 ± 0.052	3.366IleSer: 3.366 ± 0.064	3.303IleThr: 3.303 ± 0.088	3.961IleVal: 3.961 ± 0.066	0.779IleTrp: 0.779 ± 0.026	1.249IleTyr: 1.249 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	3.653LysAla: 3.653 ± 0.071	0.189LysCys: 0.189 ± 0.012	1.742LysAsp: 1.742 ± 0.047	1.361LysGlu: 1.361 ± 0.043	1.024LysPhe: 1.024 ± 0.028	2.596LysGly: 2.596 ± 0.048	0.638LysHis: 0.638 ± 0.024	1.986LysIle: 1.986 ± 0.044	1.172LysLys: 1.172 ± 0.038	3.118LysLeu: 3.118 ± 0.054	1.046LysMet: 1.046 ± 0.032	0.867LysAsn: 0.867 ± 0.03	1.753LysPro: 1.753 ± 0.052	1.009LysGln: 1.009 ± 0.03	2.153LysArg: 2.153 ± 0.049	1.877LysSer: 1.877 ± 0.053	2.12LysThr: 2.12 ± 0.046	2.208LysVal: 2.208 ± 0.054	0.362LysTrp: 0.362 ± 0.018	0.618LysTyr: 0.618 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	12.465LeuAla: 12.465 ± 0.13	1.097LeuCys: 1.097 ± 0.035	5.969LeuAsp: 5.969 ± 0.082	5.03LeuGlu: 5.03 ± 0.075	3.362LeuPhe: 3.362 ± 0.065	8.463LeuGly: 8.463 ± 0.095	2.072LeuHis: 2.072 ± 0.043	5.568LeuIle: 5.568 ± 0.088	3.148LeuLys: 3.148 ± 0.062	8.489LeuLeu: 8.489 ± 0.124	2.792LeuMet: 2.792 ± 0.058	2.779LeuAsn: 2.779 ± 0.049	5.625LeuPro: 5.625 ± 0.086	3.294LeuGln: 3.294 ± 0.05	7.246LeuArg: 7.246 ± 0.095	7.085LeuSer: 7.085 ± 0.115	6.285LeuThr: 6.285 ± 0.114	6.475LeuVal: 6.475 ± 0.083	1.24LeuTrp: 1.24 ± 0.035	1.964LeuTyr: 1.964 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	3.542MetAla: 3.542 ± 0.07	0.199MetCys: 0.199 ± 0.014	1.448MetAsp: 1.448 ± 0.041	1.289MetGlu: 1.289 ± 0.04	0.855MetPhe: 0.855 ± 0.033	2.338MetGly: 2.338 ± 0.047	0.482MetHis: 0.482 ± 0.02	1.747MetIle: 1.747 ± 0.042	1.053MetLys: 1.053 ± 0.034	2.644MetLeu: 2.644 ± 0.061	0.853MetMet: 0.853 ± 0.03	0.906MetAsn: 0.906 ± 0.029	1.576MetPro: 1.576 ± 0.037	1.152MetGln: 1.152 ± 0.03	2.013MetArg: 2.013 ± 0.048	1.817MetSer: 1.817 ± 0.041	2.205MetThr: 2.205 ± 0.046	2.035MetVal: 2.035 ± 0.038	0.254MetTrp: 0.254 ± 0.015	0.369MetTyr: 0.369 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.254AsnAla: 3.254 ± 0.064	0.271AsnCys: 0.271 ± 0.017	1.652AsnAsp: 1.652 ± 0.056	1.206AsnGlu: 1.206 ± 0.034	1.021AsnPhe: 1.021 ± 0.027	2.629AsnGly: 2.629 ± 0.073	0.547AsnHis: 0.547 ± 0.024	1.424AsnIle: 1.424 ± 0.038	0.693AsnLys: 0.693 ± 0.025	2.527AsnLeu: 2.527 ± 0.047	0.769AsnMet: 0.769 ± 0.026	0.64AsnAsn: 0.64 ± 0.025	1.786AsnPro: 1.786 ± 0.045	0.788AsnGln: 0.788 ± 0.024	1.726AsnArg: 1.726 ± 0.043	1.283AsnSer: 1.283 ± 0.041	1.499AsnThr: 1.499 ± 0.042	1.814AsnVal: 1.814 ± 0.04	0.459AsnTrp: 0.459 ± 0.018	0.69AsnTyr: 0.69 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	5.664ProAla: 5.664 ± 0.084	0.375ProCys: 0.375 ± 0.019	3.97ProAsp: 3.97 ± 0.069	3.576ProGlu: 3.576 ± 0.067	1.893ProPhe: 1.893 ± 0.047	4.165ProGly: 4.165 ± 0.069	1.054ProHis: 1.054 ± 0.028	2.483ProIle: 2.483 ± 0.05	1.789ProLys: 1.789 ± 0.047	4.485ProLeu: 4.485 ± 0.078	1.452ProMet: 1.452 ± 0.039	1.485ProAsn: 1.485 ± 0.039	2.204ProPro: 2.204 ± 0.055	2.077ProGln: 2.077 ± 0.041	2.695ProArg: 2.695 ± 0.061	2.717ProSer: 2.717 ± 0.048	2.457ProThr: 2.457 ± 0.053	4.028ProVal: 4.028 ± 0.061	0.62ProTrp: 0.62 ± 0.025	1.097ProTyr: 1.097 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	5.228GlnAla: 5.228 ± 0.082	0.241GlnCys: 0.241 ± 0.013	2.081GlnAsp: 2.081 ± 0.042	1.726GlnGlu: 1.726 ± 0.039	1.214GlnPhe: 1.214 ± 0.038	3.042GlnGly: 3.042 ± 0.064	0.7GlnHis: 0.7 ± 0.025	2.655GlnIle: 2.655 ± 0.065	1.242GlnLys: 1.242 ± 0.036	3.544GlnLeu: 3.544 ± 0.065	1.348GlnMet: 1.348 ± 0.038	1.089GlnAsn: 1.089 ± 0.03	2.001GlnPro: 2.001 ± 0.047	1.353GlnGln: 1.353 ± 0.042	2.357GlnArg: 2.357 ± 0.046	2.187GlnSer: 2.187 ± 0.046	2.3GlnThr: 2.3 ± 0.063	2.789GlnVal: 2.789 ± 0.051	0.503GlnTrp: 0.503 ± 0.019	0.669GlnTyr: 0.669 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	7.762ArgAla: 7.762 ± 0.1	0.503ArgCys: 0.503 ± 0.021	4.243ArgAsp: 4.243 ± 0.066	3.314ArgGlu: 3.314 ± 0.073	2.559ArgPhe: 2.559 ± 0.047	4.376ArgGly: 4.376 ± 0.066	1.617ArgHis: 1.617 ± 0.043	3.575ArgIle: 3.575 ± 0.052	2.263ArgLys: 2.263 ± 0.048	7.011ArgLeu: 7.011 ± 0.096	1.919ArgMet: 1.919 ± 0.041	1.785ArgAsn: 1.785 ± 0.035	3.051ArgPro: 3.051 ± 0.066	2.351ArgGln: 2.351 ± 0.047	4.572ArgArg: 4.572 ± 0.09	3.442ArgSer: 3.442 ± 0.065	2.798ArgThr: 2.798 ± 0.057	4.389ArgVal: 4.389 ± 0.071	0.894ArgTrp: 0.894 ± 0.028	1.568ArgTyr: 1.568 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	6.343SerAla: 6.343 ± 0.112	0.462SerCys: 0.462 ± 0.018	3.559SerAsp: 3.559 ± 0.067	2.797SerGlu: 2.797 ± 0.057	2.245SerPhe: 2.245 ± 0.049	5.644SerGly: 5.644 ± 0.165	1.113SerHis: 1.113 ± 0.032	2.555SerIle: 2.555 ± 0.052	1.584SerLys: 1.584 ± 0.04	5.219SerLeu: 5.219 ± 0.078	1.364SerMet: 1.364 ± 0.036	1.47SerAsn: 1.47 ± 0.037	2.451SerPro: 2.451 ± 0.051	2.022SerGln: 2.022 ± 0.045	3.24SerArg: 3.24 ± 0.052	2.88SerSer: 2.88 ± 0.061	2.758SerThr: 2.758 ± 0.056	3.996SerVal: 3.996 ± 0.101	0.716SerTrp: 0.716 ± 0.025	1.457SerTyr: 1.457 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	6.621ThrAla: 6.621 ± 0.13	0.508ThrCys: 0.508 ± 0.022	3.334ThrAsp: 3.334 ± 0.068	2.784ThrGlu: 2.784 ± 0.048	1.939ThrPhe: 1.939 ± 0.041	5.24ThrGly: 5.24 ± 0.122	1.194ThrHis: 1.194 ± 0.04	2.93ThrIle: 2.93 ± 0.067	1.527ThrLys: 1.527 ± 0.038	6.1ThrLeu: 6.1 ± 0.13	1.32ThrMet: 1.32 ± 0.034	1.392ThrAsn: 1.392 ± 0.043	3.606ThrPro: 3.606 ± 0.061	2.426ThrGln: 2.426 ± 0.068	3.609ThrArg: 3.609 ± 0.052	2.832ThrSer: 2.832 ± 0.06	3.08ThrThr: 3.08 ± 0.092	4.545ThrVal: 4.545 ± 0.135	0.686ThrTrp: 0.686 ± 0.024	1.301ThrTyr: 1.301 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	8.541ValAla: 8.541 ± 0.114	0.601ValCys: 0.601 ± 0.022	4.047ValAsp: 4.047 ± 0.082	4.04ValGlu: 4.04 ± 0.067	2.758ValPhe: 2.758 ± 0.056	5.006ValGly: 5.006 ± 0.072	1.344ValHis: 1.344 ± 0.039	4.565ValIle: 4.565 ± 0.074	2.2ValLys: 2.2 ± 0.055	7.375ValLeu: 7.375 ± 0.095	2.21ValMet: 2.21 ± 0.046	1.984ValAsn: 1.984 ± 0.044	3.479ValPro: 3.479 ± 0.052	2.437ValGln: 2.437 ± 0.057	3.719ValArg: 3.719 ± 0.06	4.366ValSer: 4.366 ± 0.118	4.997ValThr: 4.997 ± 0.121	5.318ValVal: 5.318 ± 0.076	0.908ValTrp: 0.908 ± 0.031	1.351ValTyr: 1.351 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.364TrpAla: 1.364 ± 0.041	0.136TrpCys: 0.136 ± 0.011	0.757TrpAsp: 0.757 ± 0.028	0.589TrpGlu: 0.589 ± 0.025	0.541TrpPhe: 0.541 ± 0.021	0.988TrpGly: 0.988 ± 0.028	0.38TrpHis: 0.38 ± 0.017	0.711TrpIle: 0.711 ± 0.026	0.444TrpLys: 0.444 ± 0.024	1.624TrpLeu: 1.624 ± 0.042	0.396TrpMet: 0.396 ± 0.019	0.413TrpAsn: 0.413 ± 0.02	0.694TrpPro: 0.694 ± 0.027	0.594TrpGln: 0.594 ± 0.022	1.049TrpArg: 1.049 ± 0.033	0.85TrpSer: 0.85 ± 0.027	0.734TrpThr: 0.734 ± 0.029	0.945TrpVal: 0.945 ± 0.032	0.214TrpTrp: 0.214 ± 0.014	0.252TrpTyr: 0.252 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.457TyrAla: 2.457 ± 0.042	0.23TyrCys: 0.23 ± 0.015	1.685TyrAsp: 1.685 ± 0.04	1.166TyrGlu: 1.166 ± 0.028	0.889TyrPhe: 0.889 ± 0.033	2.035TyrGly: 2.035 ± 0.046	0.515TyrHis: 0.515 ± 0.02	1.011TyrIle: 1.011 ± 0.031	0.557TyrLys: 0.557 ± 0.023	2.285TyrLeu: 2.285 ± 0.045	0.493TyrMet: 0.493 ± 0.02	0.632TyrAsn: 0.632 ± 0.024	1.054TyrPro: 1.054 ± 0.035	0.756TyrGln: 0.756 ± 0.025	1.504TyrArg: 1.504 ± 0.038	1.257TyrSer: 1.257 ± 0.034	1.143TyrThr: 1.143 ± 0.027	1.529TyrVal: 1.529 ± 0.033	0.376TyrTrp: 0.376 ± 0.015	0.577TyrTyr: 0.577 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3819 proteins (1238436 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---