Proteus phage PM 75

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Novosibovirus; Proteus virus PM75

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6NY97|A0A0F6NY97_9CAUD Endolysin OS=Proteus phage PM 75 OX=1560282 GN=PM75_0024 PE=4 SV=1
MM1 pKa = 7.5NNTMNITTMGEE12 pKa = 3.67LLEE15 pKa = 4.68AYY17 pKa = 9.85RR18 pKa = 11.84EE19 pKa = 4.25VVNEE23 pKa = 3.69YY24 pKa = 9.72EE25 pKa = 4.08PRR27 pKa = 11.84VVVQGFEE34 pKa = 4.28FEE36 pKa = 4.03PSEE39 pKa = 4.14VLEE42 pKa = 4.35EE43 pKa = 4.22LDD45 pKa = 3.63PVAFRR50 pKa = 11.84QGYY53 pKa = 10.56LDD55 pKa = 3.87FADD58 pKa = 5.01FMGIDD63 pKa = 3.74VDD65 pKa = 4.54EE66 pKa = 5.92LEE68 pKa = 5.71DD69 pKa = 4.54DD70 pKa = 3.98LL71 pKa = 6.97

Molecular weight: 8.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6NYE2|A0A0F6NYE2_9CAUD Tail tubular protein A OS=Proteus phage PM 75 OX=1560282 GN=PM75_0016 PE=4 SV=1
MM1 pKa = 7.48SNYY4 pKa = 10.19RR5 pKa = 11.84KK6 pKa = 8.44ITRR9 pKa = 11.84GQVRR13 pKa = 11.84STSLKK18 pKa = 10.2LLKK21 pKa = 10.36EE22 pKa = 3.99QGGLCAICLKK32 pKa = 10.67PIDD35 pKa = 4.51LSIKK39 pKa = 10.17GEE41 pKa = 3.99MVLDD45 pKa = 4.36HH46 pKa = 6.75SHH48 pKa = 6.66DD49 pKa = 3.44TGLIRR54 pKa = 11.84GVLHH58 pKa = 6.82RR59 pKa = 11.84SCNSSEE65 pKa = 3.99GKK67 pKa = 9.4VANAAGRR74 pKa = 11.84WGAKK78 pKa = 9.94SMDD81 pKa = 3.68YY82 pKa = 11.06KK83 pKa = 11.26DD84 pKa = 3.51IIQYY88 pKa = 10.07LKK90 pKa = 10.72QLIQYY95 pKa = 9.43LEE97 pKa = 4.23KK98 pKa = 10.84EE99 pKa = 4.44PTNLIYY105 pKa = 10.85YY106 pKa = 9.0NHH108 pKa = 6.42LTEE111 pKa = 4.31EE112 pKa = 4.39EE113 pKa = 4.15KK114 pKa = 10.82RR115 pKa = 11.84LQRR118 pKa = 11.84NAKK121 pKa = 7.3EE122 pKa = 3.92RR123 pKa = 11.84KK124 pKa = 8.79RR125 pKa = 11.84RR126 pKa = 11.84AEE128 pKa = 3.82RR129 pKa = 11.84KK130 pKa = 9.31ARR132 pKa = 11.84EE133 pKa = 3.88AVKK136 pKa = 10.79AKK138 pKa = 10.67VGG140 pKa = 3.44

Molecular weight: 16.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

10618

71

1293

424.7

47.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.544 ± 0.54

0.998 ± 0.199

5.971 ± 0.256

5.877 ± 0.3

3.4 ± 0.184

7.29 ± 0.258

1.902 ± 0.19

5.105 ± 0.286

6.046 ± 0.294

9.079 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.646 ± 0.211

4.408 ± 0.226

3.692 ± 0.225

5.02 ± 0.465

4.803 ± 0.284

7.45 ± 0.326

6.687 ± 0.298

7.026 ± 0.273

1.149 ± 0.107

3.908 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski