Enterococcus phage EFP01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Brockvirinae; Schiekvirus; Enterococcus virus EFP01

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 193 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A288TXP4|A0A288TXP4_9CAUD Uncharacterized protein OS=Enterococcus phage EFP01 OX=1926594 GN=EFP01_016 PE=4 SV=1
MM1 pKa = 7.35TNKK4 pKa = 10.03NSNIQLALANVTRR17 pKa = 11.84YY18 pKa = 10.69KK19 pKa = 11.32DD20 pKa = 3.37MNDD23 pKa = 2.92YY24 pKa = 10.19KK25 pKa = 10.55QAHH28 pKa = 5.54EE29 pKa = 4.4TPEE32 pKa = 3.7EE33 pKa = 3.94AQADD37 pKa = 4.31FNEE40 pKa = 4.9KK41 pKa = 10.06IADD44 pKa = 4.14GYY46 pKa = 9.27IQNITLSTYY55 pKa = 11.0NGATQKK61 pKa = 9.15NTFFEE66 pKa = 4.45LTDD69 pKa = 3.71EE70 pKa = 5.09NILEE74 pKa = 4.65AIYY77 pKa = 10.67TDD79 pKa = 3.39LHH81 pKa = 5.75EE82 pKa = 4.57TDD84 pKa = 4.12SYY86 pKa = 12.0SMIDD90 pKa = 3.6EE91 pKa = 4.5MEE93 pKa = 4.21EE94 pKa = 4.15STDD97 pKa = 3.56LTKK100 pKa = 10.09WDD102 pKa = 3.98YY103 pKa = 11.04IRR105 pKa = 11.84AYY107 pKa = 8.92TEE109 pKa = 3.59IYY111 pKa = 10.88NVFTLDD117 pKa = 3.12GLVYY121 pKa = 10.12IEE123 pKa = 5.02RR124 pKa = 11.84DD125 pKa = 3.15

Molecular weight: 14.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A288TY61|A0A288TY61_9CAUD Uncharacterized protein OS=Enterococcus phage EFP01 OX=1926594 GN=EFP01_081 PE=4 SV=1
MM1 pKa = 7.2TNKK4 pKa = 9.61AQHH7 pKa = 5.77IAKK10 pKa = 8.61MAKK13 pKa = 9.5EE14 pKa = 4.14MYY16 pKa = 8.84GTDD19 pKa = 4.32KK20 pKa = 10.34ITTAQLAYY28 pKa = 8.28ITDD31 pKa = 3.81MLTPSTYY38 pKa = 11.02LLRR41 pKa = 11.84NHH43 pKa = 6.35SVRR46 pKa = 11.84NHH48 pKa = 7.6PITFMISGRR57 pKa = 11.84DD58 pKa = 3.46QQKK61 pKa = 10.5AQAHH65 pKa = 6.1RR66 pKa = 11.84PWQVKK71 pKa = 9.89IINDD75 pKa = 3.31QHH77 pKa = 5.27RR78 pKa = 11.84TKK80 pKa = 10.97AVIKK84 pKa = 10.27SRR86 pKa = 11.84QLGLRR91 pKa = 11.84LAPLL95 pKa = 3.65

Molecular weight: 10.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

193

0

193

44767

42

2488

232.0

26.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.112 ± 0.226

0.918 ± 0.092

6.199 ± 0.129

7.99 ± 0.305

3.804 ± 0.115

6.248 ± 0.202

1.651 ± 0.107

6.214 ± 0.119

7.925 ± 0.211

8.312 ± 0.192

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.473 ± 0.075

5.504 ± 0.163

3.221 ± 0.138

3.824 ± 0.159

4.398 ± 0.144

5.92 ± 0.158

6.679 ± 0.263

6.882 ± 0.168

1.092 ± 0.069

4.633 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski