Amino acid dipepetide frequency for Psilocybe cyanescens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.799AlaAla: 7.799 ± 0.057
0.989AlaCys: 0.989 ± 0.013
3.882AlaAsp: 3.882 ± 0.03
4.309AlaGlu: 4.309 ± 0.035
3.21AlaPhe: 3.21 ± 0.026
5.211AlaGly: 5.211 ± 0.039
1.968AlaHis: 1.968 ± 0.019
4.3AlaIle: 4.3 ± 0.03
3.671AlaLys: 3.671 ± 0.029
7.801AlaLeu: 7.801 ± 0.047
1.739AlaMet: 1.739 ± 0.017
3.067AlaAsn: 3.067 ± 0.025
4.781AlaPro: 4.781 ± 0.038
3.172AlaGln: 3.172 ± 0.029
4.475AlaArg: 4.475 ± 0.029
7.548AlaSer: 7.548 ± 0.042
5.084AlaThr: 5.084 ± 0.032
5.092AlaVal: 5.092 ± 0.035
1.027AlaTrp: 1.027 ± 0.014
2.164AlaTyr: 2.164 ± 0.017
0.004AlaXaa: 0.004 ± 0.001
Cys
0.895CysAla: 0.895 ± 0.012
0.246CysCys: 0.246 ± 0.007
0.645CysAsp: 0.645 ± 0.011
0.554CysGlu: 0.554 ± 0.01
0.51CysPhe: 0.51 ± 0.008
0.814CysGly: 0.814 ± 0.012
0.339CysHis: 0.339 ± 0.007
0.753CysIle: 0.753 ± 0.012
0.527CysLys: 0.527 ± 0.01
1.239CysLeu: 1.239 ± 0.015
0.273CysMet: 0.273 ± 0.008
0.451CysAsn: 0.451 ± 0.01
0.716CysPro: 0.716 ± 0.013
0.428CysGln: 0.428 ± 0.009
0.678CysArg: 0.678 ± 0.012
0.996CysSer: 0.996 ± 0.015
0.76CysThr: 0.76 ± 0.013
0.778CysVal: 0.778 ± 0.011
0.181CysTrp: 0.181 ± 0.005
0.328CysTyr: 0.328 ± 0.007
0.001CysXaa: 0.001 ± 0.0
Asp
4.391AspAla: 4.391 ± 0.03
0.605AspCys: 0.605 ± 0.01
4.251AspAsp: 4.251 ± 0.039
4.121AspGlu: 4.121 ± 0.032
2.065AspPhe: 2.065 ± 0.017
3.803AspGly: 3.803 ± 0.032
1.288AspHis: 1.288 ± 0.016
3.43AspIle: 3.43 ± 0.025
2.355AspLys: 2.355 ± 0.027
4.862AspLeu: 4.862 ± 0.033
1.216AspMet: 1.216 ± 0.014
2.122AspAsn: 2.122 ± 0.019
3.23AspPro: 3.23 ± 0.023
1.674AspGln: 1.674 ± 0.016
2.715AspArg: 2.715 ± 0.024
4.352AspSer: 4.352 ± 0.027
3.07AspThr: 3.07 ± 0.022
3.717AspVal: 3.717 ± 0.027
0.836AspTrp: 0.836 ± 0.012
1.461AspTyr: 1.461 ± 0.018
0.002AspXaa: 0.002 ± 0.001
Glu
4.522GluAla: 4.522 ± 0.033
0.64GluCys: 0.64 ± 0.011
4.129GluAsp: 4.129 ± 0.042
5.228GluGlu: 5.228 ± 0.058
1.801GluPhe: 1.801 ± 0.016
3.485GluGly: 3.485 ± 0.031
1.439GluHis: 1.439 ± 0.017
3.005GluIle: 3.005 ± 0.025
3.241GluLys: 3.241 ± 0.028
4.864GluLeu: 4.864 ± 0.034
1.283GluMet: 1.283 ± 0.016
2.135GluAsn: 2.135 ± 0.018
2.454GluPro: 2.454 ± 0.026
2.146GluGln: 2.146 ± 0.022
3.656GluArg: 3.656 ± 0.039
4.037GluSer: 4.037 ± 0.03
3.026GluThr: 3.026 ± 0.02
3.297GluVal: 3.297 ± 0.028
0.843GluTrp: 0.843 ± 0.012
1.589GluTyr: 1.589 ± 0.016
0.002GluXaa: 0.002 ± 0.001
Phe
2.926PheAla: 2.926 ± 0.026
0.577PheCys: 0.577 ± 0.01
2.267PheAsp: 2.267 ± 0.021
2.03PheGlu: 2.03 ± 0.023
1.745PhePhe: 1.745 ± 0.019
2.588PheGly: 2.588 ± 0.025
0.953PheHis: 0.953 ± 0.011
2.138PheIle: 2.138 ± 0.021
1.685PheLys: 1.685 ± 0.017
3.722PheLeu: 3.722 ± 0.031
0.793PheMet: 0.793 ± 0.01
1.641PheAsn: 1.641 ± 0.017
2.067PhePro: 2.067 ± 0.018
1.297PheGln: 1.297 ± 0.014
1.903PheArg: 1.903 ± 0.018
3.465PheSer: 3.465 ± 0.028
2.217PheThr: 2.217 ± 0.02
2.479PheVal: 2.479 ± 0.023
0.59PheTrp: 0.59 ± 0.01
1.1PheTyr: 1.1 ± 0.014
0.002PheXaa: 0.002 ± 0.001
Gly
4.821GlyAla: 4.821 ± 0.037
0.774GlyCys: 0.774 ± 0.012
3.256GlyAsp: 3.256 ± 0.025
3.27GlyGlu: 3.27 ± 0.031
2.458GlyPhe: 2.458 ± 0.022
5.628GlyGly: 5.628 ± 0.059
1.725GlyHis: 1.725 ± 0.02
3.272GlyIle: 3.272 ± 0.022
3.324GlyLys: 3.324 ± 0.029
5.432GlyLeu: 5.432 ± 0.031
1.466GlyMet: 1.466 ± 0.018
2.51GlyAsn: 2.51 ± 0.026
3.159GlyPro: 3.159 ± 0.028
2.307GlyGln: 2.307 ± 0.021
3.611GlyArg: 3.611 ± 0.028
5.646GlySer: 5.646 ± 0.037
3.896GlyThr: 3.896 ± 0.03
4.089GlyVal: 4.089 ± 0.031
1.015GlyTrp: 1.015 ± 0.014
1.881GlyTyr: 1.881 ± 0.019
0.003GlyXaa: 0.003 ± 0.001
His
1.995HisAla: 1.995 ± 0.018
0.36HisCys: 0.36 ± 0.008
1.368HisAsp: 1.368 ± 0.016
1.236HisGlu: 1.236 ± 0.014
1.03HisPhe: 1.03 ± 0.012
1.582HisGly: 1.582 ± 0.018
0.984HisHis: 0.984 ± 0.017
1.564HisIle: 1.564 ± 0.016
0.958HisLys: 0.958 ± 0.012
2.504HisLeu: 2.504 ± 0.022
0.506HisMet: 0.506 ± 0.008
1.003HisAsn: 1.003 ± 0.014
1.985HisPro: 1.985 ± 0.022
1.016HisGln: 1.016 ± 0.016
1.595HisArg: 1.595 ± 0.017
2.288HisSer: 2.288 ± 0.02
1.535HisThr: 1.535 ± 0.015
1.53HisVal: 1.53 ± 0.016
0.327HisTrp: 0.327 ± 0.006
0.705HisTyr: 0.705 ± 0.011
0.001HisXaa: 0.001 ± 0.0
Ile
4.276IleAla: 4.276 ± 0.029
0.783IleCys: 0.783 ± 0.013
2.988IleAsp: 2.988 ± 0.024
2.783IleGlu: 2.783 ± 0.022
2.249IlePhe: 2.249 ± 0.021
3.058IleGly: 3.058 ± 0.023
1.382IleHis: 1.382 ± 0.015
2.917IleIle: 2.917 ± 0.022
2.284IleLys: 2.284 ± 0.02
5.223IleLeu: 5.223 ± 0.034
1.082IleMet: 1.082 ± 0.013
2.023IleAsn: 2.023 ± 0.019
3.545IlePro: 3.545 ± 0.024
2.025IleGln: 2.025 ± 0.018
2.85IleArg: 2.85 ± 0.022
4.692IleSer: 4.692 ± 0.027
3.148IleThr: 3.148 ± 0.024
3.502IleVal: 3.502 ± 0.027
0.766IleTrp: 0.766 ± 0.012
1.495IleTyr: 1.495 ± 0.015
0.002IleXaa: 0.002 ± 0.0
Lys
4.109LysAla: 4.109 ± 0.031
0.512LysCys: 0.512 ± 0.01
2.781LysAsp: 2.781 ± 0.026
3.263LysGlu: 3.263 ± 0.03
1.556LysPhe: 1.556 ± 0.016
2.84LysGly: 2.84 ± 0.028
1.182LysHis: 1.182 ± 0.016
2.43LysIle: 2.43 ± 0.02
3.254LysLys: 3.254 ± 0.033
3.997LysLeu: 3.997 ± 0.029
0.992LysMet: 0.992 ± 0.013
1.77LysAsn: 1.77 ± 0.019
2.545LysPro: 2.545 ± 0.024
1.667LysGln: 1.667 ± 0.015
3.182LysArg: 3.182 ± 0.026
3.619LysSer: 3.619 ± 0.028
2.743LysThr: 2.743 ± 0.022
2.73LysVal: 2.73 ± 0.021
0.637LysTrp: 0.637 ± 0.01
1.292LysTyr: 1.292 ± 0.015
0.001LysXaa: 0.001 ± 0.0
Leu
7.68LeuAla: 7.68 ± 0.047
1.227LeuCys: 1.227 ± 0.016
5.087LeuAsp: 5.087 ± 0.029
5.24LeuGlu: 5.24 ± 0.037
3.535LeuPhe: 3.535 ± 0.031
5.343LeuGly: 5.343 ± 0.039
2.506LeuHis: 2.506 ± 0.023
4.346LeuIle: 4.346 ± 0.033
4.302LeuLys: 4.302 ± 0.026
8.63LeuLeu: 8.63 ± 0.062
1.755LeuMet: 1.755 ± 0.016
3.444LeuAsn: 3.444 ± 0.024
6.054LeuPro: 6.054 ± 0.036
3.755LeuGln: 3.755 ± 0.028
5.47LeuArg: 5.47 ± 0.032
8.336LeuSer: 8.336 ± 0.042
5.268LeuThr: 5.268 ± 0.034
5.652LeuVal: 5.652 ± 0.037
1.184LeuTrp: 1.184 ± 0.014
2.466LeuTyr: 2.466 ± 0.02
0.003LeuXaa: 0.003 ± 0.001
Met
1.823MetAla: 1.823 ± 0.015
0.24MetCys: 0.24 ± 0.006
1.26MetAsp: 1.26 ± 0.014
1.16MetGlu: 1.16 ± 0.013
0.775MetPhe: 0.775 ± 0.012
1.302MetGly: 1.302 ± 0.016
0.547MetHis: 0.547 ± 0.009
0.985MetIle: 0.985 ± 0.013
1.0MetLys: 1.0 ± 0.013
1.812MetLeu: 1.812 ± 0.018
0.492MetMet: 0.492 ± 0.009
0.815MetAsn: 0.815 ± 0.011
1.29MetPro: 1.29 ± 0.015
0.872MetGln: 0.872 ± 0.012
1.188MetArg: 1.188 ± 0.013
2.011MetSer: 2.011 ± 0.021
1.251MetThr: 1.251 ± 0.014
1.182MetVal: 1.182 ± 0.013
0.266MetTrp: 0.266 ± 0.006
0.541MetTyr: 0.541 ± 0.009
0.001MetXaa: 0.001 ± 0.0
Asn
3.284AsnAla: 3.284 ± 0.026
0.432AsnCys: 0.432 ± 0.009
2.113AsnAsp: 2.113 ± 0.021
1.956AsnGlu: 1.956 ± 0.019
1.462AsnPhe: 1.462 ± 0.017
2.783AsnGly: 2.783 ± 0.027
0.935AsnHis: 0.935 ± 0.013
2.348AsnIle: 2.348 ± 0.021
1.659AsnLys: 1.659 ± 0.018
3.571AsnLeu: 3.571 ± 0.025
0.851AsnMet: 0.851 ± 0.013
1.865AsnAsn: 1.865 ± 0.023
2.614AsnPro: 2.614 ± 0.021
1.328AsnGln: 1.328 ± 0.016
1.884AsnArg: 1.884 ± 0.017
3.234AsnSer: 3.234 ± 0.029
2.527AsnThr: 2.527 ± 0.023
2.561AsnVal: 2.561 ± 0.02
0.502AsnTrp: 0.502 ± 0.008
0.987AsnTyr: 0.987 ± 0.012
0.002AsnXaa: 0.002 ± 0.001
Pro
5.139ProAla: 5.139 ± 0.042
0.529ProCys: 0.529 ± 0.013
3.011ProAsp: 3.011 ± 0.021
3.324ProGlu: 3.324 ± 0.027
2.265ProPhe: 2.265 ± 0.02
3.548ProGly: 3.548 ± 0.029
1.597ProHis: 1.597 ± 0.016
2.984ProIle: 2.984 ± 0.022
2.549ProLys: 2.549 ± 0.023
5.41ProLeu: 5.41 ± 0.034
1.085ProMet: 1.085 ± 0.015
2.407ProAsn: 2.407 ± 0.023
6.223ProPro: 6.223 ± 0.07
2.435ProGln: 2.435 ± 0.03
3.252ProArg: 3.252 ± 0.027
7.216ProSer: 7.216 ± 0.055
4.564ProThr: 4.564 ± 0.031
3.756ProVal: 3.756 ± 0.031
0.669ProTrp: 0.669 ± 0.01
1.574ProTyr: 1.574 ± 0.017
0.003ProXaa: 0.003 ± 0.001
Gln
3.122GlnAla: 3.122 ± 0.025
0.467GlnCys: 0.467 ± 0.01
1.923GlnAsp: 1.923 ± 0.018
2.191GlnGlu: 2.191 ± 0.02
1.333GlnPhe: 1.333 ± 0.015
2.225GlnGly: 2.225 ± 0.023
1.171GlnHis: 1.171 ± 0.016
1.94GlnIle: 1.94 ± 0.017
1.805GlnLys: 1.805 ± 0.017
3.338GlnLeu: 3.338 ± 0.026
0.827GlnMet: 0.827 ± 0.012
1.518GlnAsn: 1.518 ± 0.018
2.383GlnPro: 2.383 ± 0.025
2.462GlnGln: 2.462 ± 0.064
2.331GlnArg: 2.331 ± 0.023
3.135GlnSer: 3.135 ± 0.025
2.343GlnThr: 2.343 ± 0.021
2.069GlnVal: 2.069 ± 0.017
0.515GlnTrp: 0.515 ± 0.01
1.12GlnTyr: 1.12 ± 0.014
0.001GlnXaa: 0.001 ± 0.0
Arg
4.318ArgAla: 4.318 ± 0.028
0.683ArgCys: 0.683 ± 0.012
3.013ArgAsp: 3.013 ± 0.029
3.466ArgGlu: 3.466 ± 0.04
2.075ArgPhe: 2.075 ± 0.021
3.259ArgGly: 3.259 ± 0.029
1.54ArgHis: 1.54 ± 0.015
3.025ArgIle: 3.025 ± 0.024
3.201ArgLys: 3.201 ± 0.028
5.146ArgLeu: 5.146 ± 0.031
1.274ArgMet: 1.274 ± 0.014
2.172ArgAsn: 2.172 ± 0.017
3.39ArgPro: 3.39 ± 0.03
2.26ArgGln: 2.26 ± 0.019
4.583ArgArg: 4.583 ± 0.036
4.889ArgSer: 4.889 ± 0.034
3.467ArgThr: 3.467 ± 0.029
3.375ArgVal: 3.375 ± 0.026
0.834ArgTrp: 0.834 ± 0.011
1.582ArgTyr: 1.582 ± 0.016
0.003ArgXaa: 0.003 ± 0.001
Ser
7.099SerAla: 7.099 ± 0.037
0.942SerCys: 0.942 ± 0.015
4.448SerAsp: 4.448 ± 0.03
4.11SerGlu: 4.11 ± 0.029
3.463SerPhe: 3.463 ± 0.023
5.792SerGly: 5.792 ± 0.039
2.274SerHis: 2.274 ± 0.019
4.563SerIle: 4.563 ± 0.027
3.904SerLys: 3.904 ± 0.027
8.17SerLeu: 8.17 ± 0.044
1.779SerMet: 1.779 ± 0.017
3.496SerAsn: 3.496 ± 0.025
6.277SerPro: 6.277 ± 0.055
3.292SerGln: 3.292 ± 0.029
5.172SerArg: 5.172 ± 0.04
11.295SerSer: 11.295 ± 0.091
6.555SerThr: 6.555 ± 0.044
5.216SerVal: 5.216 ± 0.032
1.075SerTrp: 1.075 ± 0.013
2.186SerTyr: 2.186 ± 0.02
0.002SerXaa: 0.002 ± 0.001
Thr
4.941ThrAla: 4.941 ± 0.034
0.733ThrCys: 0.733 ± 0.011
2.849ThrAsp: 2.849 ± 0.023
2.795ThrGlu: 2.795 ± 0.022
2.498ThrPhe: 2.498 ± 0.021
3.813ThrGly: 3.813 ± 0.031
1.57ThrHis: 1.57 ± 0.016
3.43ThrIle: 3.43 ± 0.025
2.584ThrLys: 2.584 ± 0.02
5.832ThrLeu: 5.832 ± 0.039
1.213ThrMet: 1.213 ± 0.013
2.326ThrAsn: 2.326 ± 0.021
4.82ThrPro: 4.82 ± 0.036
2.155ThrGln: 2.155 ± 0.02
3.382ThrArg: 3.382 ± 0.025
6.252ThrSer: 6.252 ± 0.034
4.707ThrThr: 4.707 ± 0.043
3.861ThrVal: 3.861 ± 0.027
0.809ThrTrp: 0.809 ± 0.01
1.636ThrTyr: 1.636 ± 0.019
0.004ThrXaa: 0.004 ± 0.001
Val
5.013ValAla: 5.013 ± 0.031
0.82ValCys: 0.82 ± 0.011
3.763ValAsp: 3.763 ± 0.023
3.649ValGlu: 3.649 ± 0.026
2.468ValPhe: 2.468 ± 0.023
3.818ValGly: 3.818 ± 0.033
1.58ValHis: 1.58 ± 0.017
3.321ValIle: 3.321 ± 0.026
2.851ValLys: 2.851 ± 0.025
5.983ValLeu: 5.983 ± 0.041
1.281ValMet: 1.281 ± 0.014
2.338ValAsn: 2.338 ± 0.02
3.854ValPro: 3.854 ± 0.03
2.431ValGln: 2.431 ± 0.019
3.216ValArg: 3.216 ± 0.025
4.874ValSer: 4.874 ± 0.03
3.496ValThr: 3.496 ± 0.024
4.494ValVal: 4.494 ± 0.032
0.874ValTrp: 0.874 ± 0.013
1.727ValTyr: 1.727 ± 0.017
0.003ValXaa: 0.003 ± 0.001
Trp
0.978TrpAla: 0.978 ± 0.012
0.184TrpCys: 0.184 ± 0.006
0.905TrpAsp: 0.905 ± 0.014
0.779TrpGlu: 0.779 ± 0.011
0.54TrpPhe: 0.54 ± 0.01
0.831TrpGly: 0.831 ± 0.012
0.315TrpHis: 0.315 ± 0.007
0.798TrpIle: 0.798 ± 0.013
0.817TrpLys: 0.817 ± 0.012
1.174TrpLeu: 1.174 ± 0.015
0.327TrpMet: 0.327 ± 0.007
0.654TrpAsn: 0.654 ± 0.011
0.562TrpPro: 0.562 ± 0.009
0.442TrpGln: 0.442 ± 0.01
0.856TrpArg: 0.856 ± 0.011
1.044TrpSer: 1.044 ± 0.014
0.885TrpThr: 0.885 ± 0.012
0.823TrpVal: 0.823 ± 0.012
0.268TrpTrp: 0.268 ± 0.007
0.4TrpTyr: 0.4 ± 0.009
0.001TrpXaa: 0.001 ± 0.0
Tyr
2.158TyrAla: 2.158 ± 0.02
0.37TyrCys: 0.37 ± 0.008
1.625TyrAsp: 1.625 ± 0.017
1.357TyrGlu: 1.357 ± 0.017
1.144TyrPhe: 1.144 ± 0.014
1.82TyrGly: 1.82 ± 0.019
0.776TyrHis: 0.776 ± 0.01
1.521TyrIle: 1.521 ± 0.015
1.13TyrLys: 1.13 ± 0.013
2.57TyrLeu: 2.57 ± 0.022
0.577TyrMet: 0.577 ± 0.009
1.139TyrAsn: 1.139 ± 0.012
1.591TyrPro: 1.591 ± 0.019
1.051TyrGln: 1.051 ± 0.012
1.551TyrArg: 1.551 ± 0.015
2.177TyrSer: 2.177 ± 0.018
1.666TyrThr: 1.666 ± 0.018
1.648TyrVal: 1.648 ± 0.017
0.363TyrTrp: 0.363 ± 0.007
0.891TyrTyr: 0.891 ± 0.013
0.001TyrXaa: 0.001 ± 0.0
Xaa
0.003XaaAla: 0.003 ± 0.001
0.001XaaCys: 0.001 ± 0.0
0.002XaaAsp: 0.002 ± 0.0
0.002XaaGlu: 0.002 ± 0.001
0.001XaaPhe: 0.001 ± 0.0
0.003XaaGly: 0.003 ± 0.001
0.001XaaHis: 0.001 ± 0.0
0.001XaaIle: 0.001 ± 0.0
0.002XaaLys: 0.002 ± 0.0
0.003XaaLeu: 0.003 ± 0.001
0.002XaaMet: 0.002 ± 0.001
0.002XaaAsn: 0.002 ± 0.001
0.003XaaPro: 0.003 ± 0.001
0.002XaaGln: 0.002 ± 0.0
0.004XaaArg: 0.004 ± 0.001
0.004XaaSer: 0.004 ± 0.001
0.004XaaThr: 0.004 ± 0.001
0.002XaaVal: 0.002 ± 0.001
0.001XaaTrp: 0.001 ± 0.0
0.001XaaTyr: 0.001 ± 0.0
0.002XaaXaa: 0.002 ± 0.001
Statistics based on 15931 proteins (6841066 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski