Candidatus Borkfalkia ceftriaxoniphila

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Candidatus Borkfalkiales; Candidatus Borkfalkiaceae; Candidatus Borkfalkia

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2214 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2KBA1|A0A4Q2KBA1_9FIRM RNA-binding protein OS=Candidatus Borkfalkia ceftriaxoniphila OX=2508949 GN=ESZ91_02475 PE=4 SV=1
MM1 pKa = 7.47SKK3 pKa = 10.48DD4 pKa = 3.3KK5 pKa = 11.05QMHH8 pKa = 6.94DD9 pKa = 4.27EE10 pKa = 4.33EE11 pKa = 5.81CDD13 pKa = 3.6CCEE16 pKa = 3.91EE17 pKa = 4.4DD18 pKa = 4.33VNIVDD23 pKa = 5.54LVDD26 pKa = 5.42DD27 pKa = 4.15EE28 pKa = 5.54GKK30 pKa = 9.79VHH32 pKa = 6.55KK33 pKa = 9.51CYY35 pKa = 10.49HH36 pKa = 6.32IGTIEE41 pKa = 4.07YY42 pKa = 10.43KK43 pKa = 10.45NGWYY47 pKa = 10.52AFFQPADD54 pKa = 3.85EE55 pKa = 5.06EE56 pKa = 4.48EE57 pKa = 4.63DD58 pKa = 4.83DD59 pKa = 4.18EE60 pKa = 6.35DD61 pKa = 5.12SVTILQIVGEE71 pKa = 4.29EE72 pKa = 4.51GEE74 pKa = 4.39EE75 pKa = 3.83EE76 pKa = 4.28LVPVEE81 pKa = 4.8DD82 pKa = 5.88DD83 pKa = 3.53KK84 pKa = 12.03LLDD87 pKa = 3.54EE88 pKa = 4.69VFEE91 pKa = 4.26EE92 pKa = 5.25FCRR95 pKa = 11.84IMEE98 pKa = 4.3EE99 pKa = 5.6DD100 pKa = 3.5EE101 pKa = 4.94DD102 pKa = 4.78ADD104 pKa = 3.89EE105 pKa = 4.7AASLDD110 pKa = 3.79TEE112 pKa = 4.34FEE114 pKa = 4.26EE115 pKa = 6.14GCGCGCVHH123 pKa = 7.48HH124 pKa = 6.63DD125 pKa = 3.66HH126 pKa = 6.78KK127 pKa = 10.81HH128 pKa = 5.07PKK130 pKa = 8.58EE131 pKa = 4.4DD132 pKa = 3.38KK133 pKa = 10.65

Molecular weight: 15.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q2KD18|A0A4Q2KD18_9FIRM Uncharacterized protein OS=Candidatus Borkfalkia ceftriaxoniphila OX=2508949 GN=ESZ91_05370 PE=4 SV=1
MM1 pKa = 7.79CGQSLFRR8 pKa = 11.84QRR10 pKa = 11.84SLPAAPHH17 pKa = 6.6DD18 pKa = 4.02AQDD21 pKa = 3.26RR22 pKa = 11.84LRR24 pKa = 11.84RR25 pKa = 11.84LGDD28 pKa = 3.17GSVRR32 pKa = 11.84RR33 pKa = 11.84TARR36 pKa = 11.84RR37 pKa = 11.84LAALAVLLAAALVLFLVEE55 pKa = 4.75SLIPPLLPMAPYY67 pKa = 10.81VKK69 pKa = 9.93IGLANSIVLLVIVLFGARR87 pKa = 11.84DD88 pKa = 3.22AFLFVLAKK96 pKa = 10.32NLLGALFGSLFALAFNLAGSLAAYY120 pKa = 10.23AVMVLLYY127 pKa = 10.38RR128 pKa = 11.84FVFPKK133 pKa = 10.37VSLVAISVAGAVLSNVARR151 pKa = 11.84TAVGVWLMDD160 pKa = 3.77APSLFIQLPAVCIFSVCAGIVIGVLAVFLIKK191 pKa = 10.42YY192 pKa = 9.87LPEE195 pKa = 4.68RR196 pKa = 11.84LTKK199 pKa = 10.37QEE201 pKa = 3.77

Molecular weight: 21.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2214

0

2214

794369

29

2940

358.8

39.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.821 ± 0.064

1.665 ± 0.022

5.707 ± 0.04

7.125 ± 0.043

4.833 ± 0.038

7.481 ± 0.052

1.385 ± 0.019

6.225 ± 0.04

6.582 ± 0.041

8.954 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.501 ± 0.022

4.354 ± 0.04

3.288 ± 0.023

2.607 ± 0.023

4.746 ± 0.053

6.0 ± 0.035

5.28 ± 0.055

7.206 ± 0.04

0.907 ± 0.019

4.332 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski