Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Xanthobacteraceae; Azorhizobium; Azorhizobium caulinodans

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4708 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8IDJ4|A8IDJ4_AZOC5 Peptidase OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571) OX=438753 GN=AZC_2950 PE=3 SV=1
MM1 pKa = 7.8AKK3 pKa = 7.34TTTITWSWGTNSVIAFDD20 pKa = 4.23PASDD24 pKa = 3.37ILDD27 pKa = 4.09FGWFQAGQFTVSEE40 pKa = 4.61VNGSVVISIPTNNQTYY56 pKa = 8.17TLTGVTLSEE65 pKa = 4.37LSLSNILAKK74 pKa = 10.76DD75 pKa = 3.42SGAVTEE81 pKa = 4.38WTTALGGASAGSGGSSGGATSGGATSGGTSTGGSAGGSPASGTTDD126 pKa = 2.81GGTSGSSGGAGHH138 pKa = 6.78VGDD141 pKa = 4.79GLATVTTISWAWGTHH156 pKa = 3.84TALNFDD162 pKa = 3.9TALDD166 pKa = 3.72KK167 pKa = 11.51LDD169 pKa = 4.68FGWLSADD176 pKa = 3.03NFTVSEE182 pKa = 4.47VNGSVVIAIPSNSQTYY198 pKa = 8.14TLTGVTLSEE207 pKa = 4.23LSLANIVAKK216 pKa = 10.64DD217 pKa = 3.79GSALAEE223 pKa = 4.03WTTALSGHH231 pKa = 6.28SGGSSGTSGGSTGGSTSGGGSSSAGGSTSTGGGSTGGGSTGGSSTGGSTGGTSGGGTSGGDD292 pKa = 2.96AALYY296 pKa = 10.41AEE298 pKa = 4.91AWSASKK304 pKa = 10.66VYY306 pKa = 10.63QGGDD310 pKa = 2.98HH311 pKa = 6.81AAVGNNVYY319 pKa = 9.38EE320 pKa = 4.29AHH322 pKa = 7.05WWTQGTDD329 pKa = 3.3PSTHH333 pKa = 6.22NGGDD337 pKa = 3.2GSGQVWTLVGHH348 pKa = 7.46LDD350 pKa = 3.54TTTVVPNAPADD361 pKa = 3.77LFATNVSDD369 pKa = 4.32TSALLVWDD377 pKa = 3.76KK378 pKa = 11.58AVINGAGTVSAYY390 pKa = 9.99EE391 pKa = 3.88IYY393 pKa = 10.85EE394 pKa = 4.13NGTLVGTTSGTSYY407 pKa = 11.2KK408 pKa = 9.5VTGLGASTAYY418 pKa = 10.51SFTVVAVDD426 pKa = 3.7EE427 pKa = 5.24AGHH430 pKa = 6.03SPAAVPISVTTDD442 pKa = 3.36PVHH445 pKa = 6.45TSTLEE450 pKa = 3.9QTYY453 pKa = 10.58SPYY456 pKa = 10.25IDD458 pKa = 3.85MSLSTSQDD466 pKa = 3.26LLSIAKK472 pKa = 10.2ASGVTDD478 pKa = 4.04FTLAFVLSSGTDD490 pKa = 3.21TLGWGGVGTLGNDD503 pKa = 3.65TLPSGSSIHH512 pKa = 6.58DD513 pKa = 3.17QVAAVQAIGGDD524 pKa = 3.35ITISFGGANGQEE536 pKa = 3.95AALTFSSATKK546 pKa = 9.2LTAAYY551 pKa = 10.1QSVLDD556 pKa = 4.11TYY558 pKa = 10.73HH559 pKa = 6.18VNKK562 pKa = 9.86IDD564 pKa = 3.68FDD566 pKa = 4.11IEE568 pKa = 3.78GGAIANTSANHH579 pKa = 6.26LRR581 pKa = 11.84DD582 pKa = 3.38QALVALEE589 pKa = 4.25AANPDD594 pKa = 3.66LKK596 pKa = 11.33VSFTLPVLPTGLTNDD611 pKa = 3.93GLNLLKK617 pKa = 10.46QALTDD622 pKa = 3.8GVHH625 pKa = 6.56IDD627 pKa = 3.86TVNIMAMDD635 pKa = 4.02YY636 pKa = 10.56GASVDD641 pKa = 3.93SGDD644 pKa = 3.95MGTDD648 pKa = 4.03AISAAQATLAQMHH661 pKa = 5.97TLGLDD666 pKa = 3.01AKK668 pKa = 10.64LGVTVMVGMNDD679 pKa = 3.23VQSEE683 pKa = 4.72VFTLSDD689 pKa = 3.38AQQLLDD695 pKa = 3.66YY696 pKa = 10.85AQGNEE701 pKa = 4.6DD702 pKa = 3.95ISGLSIWSVGRR713 pKa = 11.84DD714 pKa = 3.13NGSTVGTVSPLGSGIQQTAYY734 pKa = 9.67EE735 pKa = 4.11FSHH738 pKa = 7.1IFGHH742 pKa = 6.8II743 pKa = 3.2

Molecular weight: 73.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8IM23|A8IM23_AZOC5 Formyl-CoA:oxalate CoA-transferase OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571) OX=438753 GN=frc PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27NGRR28 pKa = 11.84KK29 pKa = 9.24IIAARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.12GRR39 pKa = 11.84KK40 pKa = 9.3RR41 pKa = 11.84LSAA44 pKa = 4.01

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4708

0

4708

1602785

30

5585

340.4

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.567 ± 0.053

0.814 ± 0.011

5.277 ± 0.03

5.336 ± 0.045

3.626 ± 0.022

8.886 ± 0.071

1.949 ± 0.02

4.665 ± 0.027

2.929 ± 0.035

10.449 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.36 ± 0.017

2.35 ± 0.026

5.664 ± 0.039

2.978 ± 0.02

7.229 ± 0.052

5.288 ± 0.046

5.492 ± 0.061

7.796 ± 0.029

1.246 ± 0.015

2.1 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski