Amino acid dipepetide frequency for Thermogladius calderae (strain DSM 22663 / VKM B-2946 / 1633)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.527AlaAla: 5.527 ± 0.156
0.498AlaCys: 0.498 ± 0.035
2.875AlaAsp: 2.875 ± 0.083
4.644AlaGlu: 4.644 ± 0.129
2.672AlaPhe: 2.672 ± 0.094
6.381AlaGly: 6.381 ± 0.131
1.04AlaHis: 1.04 ± 0.049
4.309AlaIle: 4.309 ± 0.113
3.819AlaLys: 3.819 ± 0.119
9.707AlaLeu: 9.707 ± 0.207
1.64AlaMet: 1.64 ± 0.057
1.674AlaAsn: 1.674 ± 0.071
2.775AlaPro: 2.775 ± 0.094
1.386AlaGln: 1.386 ± 0.061
5.991AlaArg: 5.991 ± 0.132
5.634AlaSer: 5.634 ± 0.129
3.638AlaThr: 3.638 ± 0.111
8.006AlaVal: 8.006 ± 0.174
1.042AlaTrp: 1.042 ± 0.061
3.311AlaTyr: 3.311 ± 0.096
0.0AlaXaa: 0.0 ± 0.0
Cys
0.329CysAla: 0.329 ± 0.035
0.073CysCys: 0.073 ± 0.013
0.317CysAsp: 0.317 ± 0.03
0.378CysGlu: 0.378 ± 0.033
0.227CysPhe: 0.227 ± 0.023
0.859CysGly: 0.859 ± 0.056
0.137CysHis: 0.137 ± 0.016
0.276CysIle: 0.276 ± 0.025
0.232CysLys: 0.232 ± 0.026
0.632CysLeu: 0.632 ± 0.042
0.081CysMet: 0.081 ± 0.012
0.178CysAsn: 0.178 ± 0.023
0.673CysPro: 0.673 ± 0.056
0.139CysGln: 0.139 ± 0.019
0.591CysArg: 0.591 ± 0.04
0.51CysSer: 0.51 ± 0.04
0.286CysThr: 0.286 ± 0.028
0.608CysVal: 0.608 ± 0.04
0.107CysTrp: 0.107 ± 0.018
0.224CysTyr: 0.224 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
3.16AspAla: 3.16 ± 0.084
0.3AspCys: 0.3 ± 0.028
1.73AspAsp: 1.73 ± 0.069
3.655AspGlu: 3.655 ± 0.099
1.857AspPhe: 1.857 ± 0.069
2.577AspGly: 2.577 ± 0.084
0.676AspHis: 0.676 ± 0.041
2.848AspIle: 2.848 ± 0.086
2.721AspLys: 2.721 ± 0.096
5.315AspLeu: 5.315 ± 0.099
0.9AspMet: 0.9 ± 0.048
1.115AspAsn: 1.115 ± 0.055
2.914AspPro: 2.914 ± 0.091
0.766AspGln: 0.766 ± 0.047
2.533AspArg: 2.533 ± 0.08
2.25AspSer: 2.25 ± 0.08
1.82AspThr: 1.82 ± 0.072
5.205AspVal: 5.205 ± 0.12
0.661AspTrp: 0.661 ± 0.043
2.523AspTyr: 2.523 ± 0.084
0.0AspXaa: 0.0 ± 0.0
Glu
6.127GluAla: 6.127 ± 0.135
0.41GluCys: 0.41 ± 0.035
3.043GluAsp: 3.043 ± 0.087
6.537GluGlu: 6.537 ± 0.139
2.35GluPhe: 2.35 ± 0.08
4.8GluGly: 4.8 ± 0.115
1.071GluHis: 1.071 ± 0.052
3.716GluIle: 3.716 ± 0.111
4.834GluLys: 4.834 ± 0.142
7.889GluLeu: 7.889 ± 0.161
1.281GluMet: 1.281 ± 0.062
2.079GluAsn: 2.079 ± 0.074
2.54GluPro: 2.54 ± 0.087
1.186GluGln: 1.186 ± 0.054
4.815GluArg: 4.815 ± 0.144
3.524GluSer: 3.524 ± 0.094
2.992GluThr: 2.992 ± 0.079
7.611GluVal: 7.611 ± 0.174
0.774GluTrp: 0.774 ± 0.039
2.857GluTyr: 2.857 ± 0.09
0.0GluXaa: 0.0 ± 0.0
Phe
2.362PheAla: 2.362 ± 0.087
0.281PheCys: 0.281 ± 0.025
1.762PheAsp: 1.762 ± 0.068
2.35PheGlu: 2.35 ± 0.07
1.337PhePhe: 1.337 ± 0.06
2.184PheGly: 2.184 ± 0.085
0.588PheHis: 0.588 ± 0.035
2.218PheIle: 2.218 ± 0.075
2.528PheLys: 2.528 ± 0.084
3.36PheLeu: 3.36 ± 0.099
0.883PheMet: 0.883 ± 0.042
1.481PheAsn: 1.481 ± 0.067
1.176PhePro: 1.176 ± 0.052
0.703PheGln: 0.703 ± 0.034
2.038PheArg: 2.038 ± 0.08
2.367PheSer: 2.367 ± 0.074
2.267PheThr: 2.267 ± 0.086
3.424PheVal: 3.424 ± 0.092
0.351PheTrp: 0.351 ± 0.032
1.845PheTyr: 1.845 ± 0.063
0.0PheXaa: 0.0 ± 0.0
Gly
4.636GlyAla: 4.636 ± 0.113
0.683GlyCys: 0.683 ± 0.051
3.758GlyAsp: 3.758 ± 0.107
5.273GlyGlu: 5.273 ± 0.116
4.107GlyPhe: 4.107 ± 0.109
6.127GlyGly: 6.127 ± 0.134
1.144GlyHis: 1.144 ± 0.062
3.826GlyIle: 3.826 ± 0.09
3.851GlyLys: 3.851 ± 0.106
10.073GlyLeu: 10.073 ± 0.176
1.503GlyMet: 1.503 ± 0.069
1.779GlyAsn: 1.779 ± 0.071
2.318GlyPro: 2.318 ± 0.082
1.162GlyGln: 1.162 ± 0.058
5.29GlyArg: 5.29 ± 0.137
4.839GlySer: 4.839 ± 0.109
2.714GlyThr: 2.714 ± 0.098
9.509GlyVal: 9.509 ± 0.196
1.086GlyTrp: 1.086 ± 0.055
3.38GlyTyr: 3.38 ± 0.098
0.0GlyXaa: 0.0 ± 0.0
His
1.142HisAla: 1.142 ± 0.053
0.122HisCys: 0.122 ± 0.02
0.695HisAsp: 0.695 ± 0.038
1.035HisGlu: 1.035 ± 0.055
0.5HisPhe: 0.5 ± 0.039
1.44HisGly: 1.44 ± 0.061
0.31HisHis: 0.31 ± 0.032
0.864HisIle: 0.864 ± 0.049
0.632HisLys: 0.632 ± 0.04
1.501HisLeu: 1.501 ± 0.057
0.303HisMet: 0.303 ± 0.028
0.427HisAsn: 0.427 ± 0.032
1.03HisPro: 1.03 ± 0.052
0.295HisGln: 0.295 ± 0.028
0.9HisArg: 0.9 ± 0.05
0.822HisSer: 0.822 ± 0.042
0.6HisThr: 0.6 ± 0.039
1.586HisVal: 1.586 ± 0.064
0.173HisTrp: 0.173 ± 0.021
0.654HisTyr: 0.654 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
5.434IleAla: 5.434 ± 0.138
0.286IleCys: 0.286 ± 0.027
2.887IleAsp: 2.887 ± 0.099
3.807IleGlu: 3.807 ± 0.098
1.413IlePhe: 1.413 ± 0.061
4.466IleGly: 4.466 ± 0.124
0.998IleHis: 0.998 ± 0.049
3.001IleIle: 3.001 ± 0.095
2.85IleLys: 2.85 ± 0.109
5.254IleLeu: 5.254 ± 0.115
1.013IleMet: 1.013 ± 0.053
1.584IleAsn: 1.584 ± 0.073
3.053IlePro: 3.053 ± 0.088
1.049IleGln: 1.049 ± 0.058
3.111IleArg: 3.111 ± 0.098
3.216IleSer: 3.216 ± 0.093
2.867IleThr: 2.867 ± 0.09
6.179IleVal: 6.179 ± 0.101
0.388IleTrp: 0.388 ± 0.029
1.986IleTyr: 1.986 ± 0.066
0.0IleXaa: 0.0 ± 0.0
Lys
4.392LysAla: 4.392 ± 0.115
0.517LysCys: 0.517 ± 0.04
2.084LysAsp: 2.084 ± 0.067
3.375LysGlu: 3.375 ± 0.098
1.362LysPhe: 1.362 ± 0.056
3.997LysGly: 3.997 ± 0.102
0.857LysHis: 0.857 ± 0.044
3.206LysIle: 3.206 ± 0.095
3.292LysLys: 3.292 ± 0.106
5.861LysLeu: 5.861 ± 0.147
1.176LysMet: 1.176 ± 0.052
1.501LysAsn: 1.501 ± 0.061
3.004LysPro: 3.004 ± 0.092
1.061LysGln: 1.061 ± 0.055
3.895LysArg: 3.895 ± 0.115
3.028LysSer: 3.028 ± 0.088
3.099LysThr: 3.099 ± 0.084
5.176LysVal: 5.176 ± 0.119
0.644LysTrp: 0.644 ± 0.046
2.552LysTyr: 2.552 ± 0.094
0.0LysXaa: 0.0 ± 0.0
Leu
10.344LeuAla: 10.344 ± 0.214
0.498LeuCys: 0.498 ± 0.037
5.478LeuAsp: 5.478 ± 0.128
8.101LeuGlu: 8.101 ± 0.151
3.609LeuPhe: 3.609 ± 0.111
8.455LeuGly: 8.455 ± 0.178
1.545LeuHis: 1.545 ± 0.063
5.327LeuIle: 5.327 ± 0.121
6.488LeuLys: 6.488 ± 0.142
11.981LeuLeu: 11.981 ± 0.228
2.194LeuMet: 2.194 ± 0.082
3.433LeuAsn: 3.433 ± 0.111
4.405LeuPro: 4.405 ± 0.094
1.818LeuGln: 1.818 ± 0.067
7.616LeuArg: 7.616 ± 0.18
8.04LeuSer: 8.04 ± 0.162
5.483LeuThr: 5.483 ± 0.128
12.013LeuVal: 12.013 ± 0.173
0.971LeuTrp: 0.971 ± 0.047
4.417LeuTyr: 4.417 ± 0.114
0.0LeuXaa: 0.0 ± 0.0
Met
1.53MetAla: 1.53 ± 0.064
0.112MetCys: 0.112 ± 0.018
0.854MetAsp: 0.854 ± 0.048
1.276MetGlu: 1.276 ± 0.06
0.625MetPhe: 0.625 ± 0.041
1.642MetGly: 1.642 ± 0.068
0.261MetHis: 0.261 ± 0.023
1.157MetIle: 1.157 ± 0.058
1.137MetLys: 1.137 ± 0.056
1.981MetLeu: 1.981 ± 0.082
0.376MetMet: 0.376 ± 0.031
0.539MetAsn: 0.539 ± 0.03
1.003MetPro: 1.003 ± 0.054
0.229MetGln: 0.229 ± 0.022
1.532MetArg: 1.532 ± 0.059
1.718MetSer: 1.718 ± 0.063
0.935MetThr: 0.935 ± 0.045
2.064MetVal: 2.064 ± 0.069
0.176MetTrp: 0.176 ± 0.019
0.752MetTyr: 0.752 ± 0.046
0.0MetXaa: 0.0 ± 0.0
Asn
2.206AsnAla: 2.206 ± 0.084
0.173AsnCys: 0.173 ± 0.021
0.883AsnAsp: 0.883 ± 0.048
1.513AsnGlu: 1.513 ± 0.06
0.766AsnPhe: 0.766 ± 0.046
1.774AsnGly: 1.774 ± 0.074
0.322AsnHis: 0.322 ± 0.027
2.086AsnIle: 2.086 ± 0.071
1.467AsnLys: 1.467 ± 0.061
2.977AsnLeu: 2.977 ± 0.096
0.632AsnMet: 0.632 ± 0.038
0.874AsnAsn: 0.874 ± 0.05
2.184AsnPro: 2.184 ± 0.082
0.527AsnGln: 0.527 ± 0.039
1.374AsnArg: 1.374 ± 0.062
1.484AsnSer: 1.484 ± 0.075
1.657AsnThr: 1.657 ± 0.074
2.894AsnVal: 2.894 ± 0.095
0.339AsnTrp: 0.339 ± 0.028
1.237AsnTyr: 1.237 ± 0.056
0.0AsnXaa: 0.0 ± 0.0
Pro
2.943ProAla: 2.943 ± 0.088
0.334ProCys: 0.334 ± 0.033
2.416ProAsp: 2.416 ± 0.085
3.336ProGlu: 3.336 ± 0.111
1.791ProPhe: 1.791 ± 0.062
4.214ProGly: 4.214 ± 0.116
0.803ProHis: 0.803 ± 0.048
2.13ProIle: 2.13 ± 0.077
1.559ProLys: 1.559 ± 0.059
4.919ProLeu: 4.919 ± 0.1
0.786ProMet: 0.786 ± 0.045
1.032ProAsn: 1.032 ± 0.055
2.945ProPro: 2.945 ± 0.096
0.993ProGln: 0.993 ± 0.046
2.953ProArg: 2.953 ± 0.092
3.389ProSer: 3.389 ± 0.103
2.155ProThr: 2.155 ± 0.105
4.797ProVal: 4.797 ± 0.11
0.703ProTrp: 0.703 ± 0.044
1.986ProTyr: 1.986 ± 0.074
0.0ProXaa: 0.0 ± 0.0
Gln
1.769GlnAla: 1.769 ± 0.081
0.098GlnCys: 0.098 ± 0.015
0.612GlnAsp: 0.612 ± 0.035
1.059GlnGlu: 1.059 ± 0.05
0.534GlnPhe: 0.534 ± 0.037
1.471GlnGly: 1.471 ± 0.061
0.256GlnHis: 0.256 ± 0.026
0.888GlnIle: 0.888 ± 0.048
0.756GlnLys: 0.756 ± 0.047
2.008GlnLeu: 2.008 ± 0.062
0.376GlnMet: 0.376 ± 0.03
0.429GlnAsn: 0.429 ± 0.036
1.171GlnPro: 1.171 ± 0.059
0.598GlnGln: 0.598 ± 0.044
1.015GlnArg: 1.015 ± 0.052
0.952GlnSer: 0.952 ± 0.048
0.822GlnThr: 0.822 ± 0.055
1.923GlnVal: 1.923 ± 0.057
0.21GlnTrp: 0.21 ± 0.022
0.752GlnTyr: 0.752 ± 0.051
0.0GlnXaa: 0.0 ± 0.0
Arg
4.9ArgAla: 4.9 ± 0.104
0.5ArgCys: 0.5 ± 0.046
3.341ArgAsp: 3.341 ± 0.089
5.808ArgGlu: 5.808 ± 0.135
2.225ArgPhe: 2.225 ± 0.076
5.835ArgGly: 5.835 ± 0.145
1.064ArgHis: 1.064 ± 0.053
3.319ArgIle: 3.319 ± 0.099
2.965ArgLys: 2.965 ± 0.099
8.08ArgLeu: 8.08 ± 0.156
1.247ArgMet: 1.247 ± 0.053
1.359ArgAsn: 1.359 ± 0.054
2.958ArgPro: 2.958 ± 0.097
1.079ArgGln: 1.079 ± 0.049
5.288ArgArg: 5.288 ± 0.156
3.472ArgSer: 3.472 ± 0.1
2.152ArgThr: 2.152 ± 0.073
8.219ArgVal: 8.219 ± 0.156
0.632ArgTrp: 0.632 ± 0.039
2.15ArgTyr: 2.15 ± 0.078
0.0ArgXaa: 0.0 ± 0.0
Ser
3.885SerAla: 3.885 ± 0.111
0.422SerCys: 0.422 ± 0.037
2.516SerAsp: 2.516 ± 0.087
3.804SerGlu: 3.804 ± 0.107
2.326SerPhe: 2.326 ± 0.066
5.124SerGly: 5.124 ± 0.108
0.91SerHis: 0.91 ± 0.044
4.307SerIle: 4.307 ± 0.109
3.594SerLys: 3.594 ± 0.093
7.677SerLeu: 7.677 ± 0.157
1.608SerMet: 1.608 ± 0.061
1.811SerAsn: 1.811 ± 0.076
3.036SerPro: 3.036 ± 0.081
1.181SerGln: 1.181 ± 0.061
4.846SerArg: 4.846 ± 0.109
4.617SerSer: 4.617 ± 0.127
3.448SerThr: 3.448 ± 0.104
5.764SerVal: 5.764 ± 0.13
0.727SerTrp: 0.727 ± 0.037
2.482SerTyr: 2.482 ± 0.099
0.0SerXaa: 0.0 ± 0.0
Thr
3.585ThrAla: 3.585 ± 0.111
0.322ThrCys: 0.322 ± 0.032
1.562ThrAsp: 1.562 ± 0.06
2.062ThrGlu: 2.062 ± 0.068
1.418ThrPhe: 1.418 ± 0.058
4.81ThrGly: 4.81 ± 0.12
0.81ThrHis: 0.81 ± 0.043
2.831ThrIle: 2.831 ± 0.089
1.794ThrLys: 1.794 ± 0.066
5.554ThrLeu: 5.554 ± 0.125
0.837ThrMet: 0.837 ± 0.045
1.122ThrAsn: 1.122 ± 0.062
3.153ThrPro: 3.153 ± 0.098
0.91ThrGln: 0.91 ± 0.051
3.053ThrArg: 3.053 ± 0.084
3.348ThrSer: 3.348 ± 0.121
3.287ThrThr: 3.287 ± 0.227
4.666ThrVal: 4.666 ± 0.125
0.576ThrTrp: 0.576 ± 0.037
1.884ThrTyr: 1.884 ± 0.086
0.0ThrXaa: 0.0 ± 0.0
Val
8.363ValAla: 8.363 ± 0.154
0.774ValCys: 0.774 ± 0.045
6.218ValAsp: 6.218 ± 0.118
9.229ValGlu: 9.229 ± 0.19
4.265ValPhe: 4.265 ± 0.106
6.784ValGly: 6.784 ± 0.155
1.471ValHis: 1.471 ± 0.065
5.581ValIle: 5.581 ± 0.125
6.889ValLys: 6.889 ± 0.139
11.44ValLeu: 11.44 ± 0.193
1.869ValMet: 1.869 ± 0.06
3.131ValAsn: 3.131 ± 0.092
3.492ValPro: 3.492 ± 0.097
1.603ValGln: 1.603 ± 0.059
6.235ValArg: 6.235 ± 0.147
7.067ValSer: 7.067 ± 0.156
4.414ValThr: 4.414 ± 0.115
13.292ValVal: 13.292 ± 0.223
0.959ValTrp: 0.959 ± 0.049
5.261ValTyr: 5.261 ± 0.122
0.0ValXaa: 0.0 ± 0.0
Trp
0.805TrpAla: 0.805 ± 0.045
0.083TrpCys: 0.083 ± 0.015
0.566TrpAsp: 0.566 ± 0.038
0.659TrpGlu: 0.659 ± 0.037
0.561TrpPhe: 0.561 ± 0.036
0.844TrpGly: 0.844 ± 0.05
0.178TrpHis: 0.178 ± 0.021
0.581TrpIle: 0.581 ± 0.045
0.493TrpLys: 0.493 ± 0.035
1.342TrpLeu: 1.342 ± 0.059
0.295TrpMet: 0.295 ± 0.023
0.337TrpAsn: 0.337 ± 0.034
0.361TrpPro: 0.361 ± 0.028
0.195TrpGln: 0.195 ± 0.019
0.91TrpArg: 0.91 ± 0.049
0.883TrpSer: 0.883 ± 0.04
0.427TrpThr: 0.427 ± 0.031
1.086TrpVal: 1.086 ± 0.045
0.229TrpTrp: 0.229 ± 0.026
0.459TrpTyr: 0.459 ± 0.036
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.128TyrAla: 3.128 ± 0.095
0.371TyrCys: 0.371 ± 0.03
1.93TyrAsp: 1.93 ± 0.072
2.535TyrGlu: 2.535 ± 0.082
1.559TyrPhe: 1.559 ± 0.064
3.275TyrGly: 3.275 ± 0.104
0.591TyrHis: 0.591 ± 0.038
2.369TyrIle: 2.369 ± 0.074
2.179TyrLys: 2.179 ± 0.076
4.668TyrLeu: 4.668 ± 0.111
0.898TyrMet: 0.898 ± 0.045
1.484TyrAsn: 1.484 ± 0.063
1.996TyrPro: 1.996 ± 0.076
0.82TyrGln: 0.82 ± 0.048
2.606TyrArg: 2.606 ± 0.083
2.923TyrSer: 2.923 ± 0.087
2.518TyrThr: 2.518 ± 0.084
4.314TyrVal: 4.314 ± 0.098
0.547TyrTrp: 0.547 ± 0.037
2.121TyrTyr: 2.121 ± 0.093
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1414 proteins (409803 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski