Muribacter muris

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2190 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J5S163|A0A0J5S163_9PAST Uncharacterized protein OS=Muribacter muris OX=67855 GN=RO21_10775 PE=4 SV=1
MM1 pKa = 8.32SEE3 pKa = 4.11MNIPLIFTDD12 pKa = 3.81AAANKK17 pKa = 9.16VKK19 pKa = 10.76SLIEE23 pKa = 4.5GEE25 pKa = 4.46DD26 pKa = 3.6NPNLRR31 pKa = 11.84LRR33 pKa = 11.84VYY35 pKa = 9.12ITGGGCSGFQYY46 pKa = 11.03GFTFDD51 pKa = 4.83DD52 pKa = 3.68QVNDD56 pKa = 3.5GDD58 pKa = 4.74LTIEE62 pKa = 4.06NQNVGLVVDD71 pKa = 4.64PMSLQYY77 pKa = 11.06LIGGTVDD84 pKa = 3.54YY85 pKa = 11.18IEE87 pKa = 4.62GLEE90 pKa = 4.0GSRR93 pKa = 11.84FIVNNPNASSTCGCGSSFSII113 pKa = 5.02

Molecular weight: 12.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J5P9H0|A0A0J5P9H0_9PAST Ferrous iron transporter OS=Muribacter muris OX=67855 GN=fieF PE=4 SV=1
MM1 pKa = 7.71LLLKK5 pKa = 10.54LQIRR9 pKa = 11.84PLLQTVRR16 pKa = 11.84QKK18 pKa = 10.7PQAHH22 pKa = 5.94RR23 pKa = 11.84QMQRR27 pKa = 11.84TAPQLKK33 pKa = 9.26PIHH36 pKa = 5.91LQPPQVLQQLPHH48 pKa = 4.65QTQRR52 pKa = 11.84PLQTVRR58 pKa = 11.84QQRR61 pKa = 11.84QVPPQVQQTALQIKK75 pKa = 9.63PILQQLPHH83 pKa = 6.5QIQHH87 pKa = 7.13LLQIVRR93 pKa = 11.84QQRR96 pKa = 11.84QVPLQVQRR104 pKa = 11.84IALQLKK110 pKa = 9.53RR111 pKa = 11.84VLPQLPHH118 pKa = 5.96QIQRR122 pKa = 11.84LLQIVQQKK130 pKa = 9.76

Molecular weight: 15.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2190

0

2190

667018

25

1807

304.6

33.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.134 ± 0.062

1.029 ± 0.016

4.976 ± 0.035

6.01 ± 0.054

4.415 ± 0.044

6.696 ± 0.052

2.232 ± 0.023

6.882 ± 0.051

5.741 ± 0.05

10.778 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.026

4.414 ± 0.04

3.938 ± 0.03

5.137 ± 0.047

4.702 ± 0.047

5.56 ± 0.04

5.127 ± 0.037

6.471 ± 0.046

1.194 ± 0.019

3.205 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski