Hypholoma sublateritium (strain FD-334 SS-4)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Strophariaceae; Hypholoma; Hypholoma sublateritium

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17638 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D2KQ62|A0A0D2KQ62_HYPSF Uncharacterized protein OS=Hypholoma sublateritium (strain FD-334 SS-4) OX=945553 GN=HYPSUDRAFT_71116 PE=4 SV=1
MM1 pKa = 7.15PQVPARR7 pKa = 11.84PPPRR11 pKa = 11.84SSVPTEE17 pKa = 3.94SVFDD21 pKa = 4.64DD22 pKa = 4.28DD23 pKa = 4.46AASAFSFSIYY33 pKa = 10.44EE34 pKa = 3.9VDD36 pKa = 4.7LGDD39 pKa = 3.88ARR41 pKa = 11.84SGGSSSSASAYY52 pKa = 8.82SQPSFDD58 pKa = 4.38AFAAHH63 pKa = 7.21DD64 pKa = 3.76VAFDD68 pKa = 4.06LDD70 pKa = 4.1GGMMLPVSLPATPNDD85 pKa = 3.34IDD87 pKa = 6.24DD88 pKa = 3.8LTGWDD93 pKa = 4.29DD94 pKa = 3.51EE95 pKa = 5.02VEE97 pKa = 4.36FVFDD101 pKa = 3.94EE102 pKa = 4.35EE103 pKa = 4.64EE104 pKa = 4.74EE105 pKa = 4.08IHH107 pKa = 8.03SMDD110 pKa = 3.49LEE112 pKa = 4.32IYY114 pKa = 10.07IVRR117 pKa = 11.84TTINLMNSAGIYY129 pKa = 9.54IVRR132 pKa = 11.84TTRR135 pKa = 11.84DD136 pKa = 3.53DD137 pKa = 3.77YY138 pKa = 11.49YY139 pKa = 9.96EE140 pKa = 4.46TYY142 pKa = 10.62SSYY145 pKa = 10.75IYY147 pKa = 9.32RR148 pKa = 11.84TQVQQ152 pKa = 3.14

Molecular weight: 16.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D2PAU8|A0A0D2PAU8_HYPSF Uncharacterized protein OS=Hypholoma sublateritium (strain FD-334 SS-4) OX=945553 GN=HYPSUDRAFT_34322 PE=4 SV=1
MM1 pKa = 7.45AVSTPQTPLCHH12 pKa = 6.88ALLTTHH18 pKa = 6.83SARR21 pKa = 11.84TAPARR26 pKa = 11.84ASAATSRR33 pKa = 11.84ARR35 pKa = 11.84ASAASSTASPTRR47 pKa = 11.84ASTASARR54 pKa = 11.84SARR57 pKa = 11.84RR58 pKa = 11.84ASSAGRR64 pKa = 11.84TAARR68 pKa = 11.84TRR70 pKa = 11.84ATPPPPLPTPPSRR83 pKa = 11.84RR84 pKa = 11.84PPSPSGAQVHH94 pKa = 5.05RR95 pKa = 11.84RR96 pKa = 11.84RR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84TRR102 pKa = 11.84PAPRR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 11.84RR109 pKa = 11.84PPPPPPRR116 pKa = 11.84CTPSRR121 pKa = 11.84STRR124 pKa = 11.84TRR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84RR129 pKa = 11.84GTTPCTTPRR138 pKa = 11.84PARR141 pKa = 11.84SCRR144 pKa = 11.84TRR146 pKa = 11.84TPRR149 pKa = 11.84RR150 pKa = 11.84RR151 pKa = 11.84ARR153 pKa = 11.84APRR156 pKa = 11.84ATRR159 pKa = 11.84PRR161 pKa = 11.84LPMRR165 pKa = 11.84PRR167 pKa = 11.84TRR169 pKa = 11.84RR170 pKa = 11.84TGSRR174 pKa = 11.84SCRR177 pKa = 11.84TTSTPGGTRR186 pKa = 11.84RR187 pKa = 11.84SRR189 pKa = 11.84TTRR192 pKa = 11.84RR193 pKa = 11.84CTPRR197 pKa = 11.84SNSRR201 pKa = 11.84RR202 pKa = 11.84GRR204 pKa = 11.84RR205 pKa = 11.84PQARR209 pKa = 11.84RR210 pKa = 11.84PPPPLRR216 pKa = 11.84LHH218 pKa = 5.71PQRR221 pKa = 11.84LRR223 pKa = 11.84RR224 pKa = 11.84TRR226 pKa = 11.84PRR228 pKa = 11.84RR229 pKa = 11.84RR230 pKa = 11.84RR231 pKa = 11.84SAPLIPQAHH240 pKa = 5.48RR241 pKa = 11.84TPRR244 pKa = 11.84RR245 pKa = 11.84RR246 pKa = 11.84ARR248 pKa = 11.84SRR250 pKa = 11.84RR251 pKa = 11.84RR252 pKa = 11.84RR253 pKa = 11.84RR254 pKa = 11.84RR255 pKa = 11.84PLTEE259 pKa = 3.54MGRR262 pKa = 11.84SARR265 pKa = 11.84AWARR269 pKa = 11.84TASRR273 pKa = 11.84ARR275 pKa = 11.84RR276 pKa = 11.84RR277 pKa = 11.84RR278 pKa = 11.84RR279 pKa = 11.84ARR281 pKa = 11.84RR282 pKa = 11.84ARR284 pKa = 11.84GGVRR288 pKa = 11.84RR289 pKa = 11.84RR290 pKa = 11.84MHH292 pKa = 6.3SLSHH296 pKa = 6.9PPLPSPLPLPIRR308 pKa = 11.84RR309 pKa = 11.84RR310 pKa = 11.84MAARR314 pKa = 11.84RR315 pKa = 11.84RR316 pKa = 11.84GEE318 pKa = 3.85QEE320 pKa = 3.6EE321 pKa = 4.82GG322 pKa = 3.27

Molecular weight: 36.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17633

5

17638

6809349

49

3938

386.1

42.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.328 ± 0.026

1.369 ± 0.008

5.38 ± 0.015

5.426 ± 0.02

3.742 ± 0.011

6.34 ± 0.019

2.688 ± 0.01

5.033 ± 0.015

4.272 ± 0.016

9.122 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.024 ± 0.007

3.485 ± 0.01

6.767 ± 0.023

3.647 ± 0.014

6.458 ± 0.017

8.512 ± 0.025

6.152 ± 0.014

6.186 ± 0.015

1.387 ± 0.006

2.662 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski