Colombian potato soil-borne virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Pomovirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U3CCW0|A0A0U3CCW0_9VIRU Putative coat protein OS=Colombian potato soil-borne virus OX=1758139 GN=CP-RT PE=4 SV=1
MM1 pKa = 8.05DD2 pKa = 5.53PPVIIHH8 pKa = 6.5SPNCSCQYY16 pKa = 10.23CSSEE20 pKa = 4.19LPSTHH25 pKa = 6.3TCGSQDD31 pKa = 4.7PIAQSHH37 pKa = 6.53AGGIAARR44 pKa = 11.84ITGANSEE51 pKa = 4.5YY52 pKa = 10.67FSLSYY57 pKa = 10.88VLLVAVVSVLLGFSACVYY75 pKa = 9.59IKK77 pKa = 10.56SVSNEE82 pKa = 3.81DD83 pKa = 3.85PADD86 pKa = 3.38MTYY89 pKa = 10.84YY90 pKa = 10.6YY91 pKa = 10.77QDD93 pKa = 3.82LNSVEE98 pKa = 4.15IKK100 pKa = 10.47LGKK103 pKa = 10.1NPLDD107 pKa = 3.71PEE109 pKa = 4.51VIKK112 pKa = 10.69AIHH115 pKa = 6.28SFQEE119 pKa = 4.18YY120 pKa = 8.77PFGYY124 pKa = 9.8VPSIRR129 pKa = 11.84GGPEE133 pKa = 3.46HH134 pKa = 6.74EE135 pKa = 4.48VSNEE139 pKa = 3.4GSGAVALTDD148 pKa = 3.43SRR150 pKa = 11.84NVRR153 pKa = 11.84QVDD156 pKa = 3.93DD157 pKa = 4.37SPCAHH162 pKa = 6.39STLTSLWKK170 pKa = 10.25DD171 pKa = 3.37DD172 pKa = 4.21LSFTIIAVTVLALGVVFAPRR192 pKa = 3.2

Molecular weight: 20.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U3ALY5|A0A0U3ALY5_9VIRU Methyltransferase/RNA helicase OS=Colombian potato soil-borne virus OX=1758139 GN=ORF2 PE=4 SV=1
MM1 pKa = 6.98VRR3 pKa = 11.84SNEE6 pKa = 3.43IGARR10 pKa = 11.84PNKK13 pKa = 8.92YY14 pKa = 8.3WPVVAAVVAICLFGFLTLTNQKK36 pKa = 10.04HH37 pKa = 4.89ATQSGDD43 pKa = 3.78NIHH46 pKa = 6.78KK47 pKa = 9.54FANGGQYY54 pKa = 10.13RR55 pKa = 11.84DD56 pKa = 3.35GSKK59 pKa = 10.37SIKK62 pKa = 9.99YY63 pKa = 9.27NCNNPRR69 pKa = 11.84AYY71 pKa = 10.55NGSSSNNTFSQLFLPVLLLGAALYY95 pKa = 10.43AYY97 pKa = 10.64LWFTRR102 pKa = 11.84PDD104 pKa = 3.34CSVTCRR110 pKa = 11.84GDD112 pKa = 3.17CCKK115 pKa = 10.85NYY117 pKa = 10.53GGQQQ121 pKa = 3.0

Molecular weight: 13.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

1

7

5012

121

1850

716.0

80.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.003 ± 0.585

1.816 ± 0.164

6.584 ± 0.504

7.482 ± 0.409

4.01 ± 0.214

6.524 ± 0.46

1.856 ± 0.164

4.569 ± 0.131

6.265 ± 0.737

9.058 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.388

4.27 ± 0.339

3.571 ± 0.291

3.272 ± 0.299

6.006 ± 0.36

7.941 ± 0.628

5.188 ± 0.159

7.841 ± 0.45

1.057 ± 0.104

3.392 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski