Rice grassy stunt tenuivirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Tenuivirus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O89174|O89174_9VIRU p4V protein OS=Rice grassy stunt tenuivirus OX=66266 GN=p4V PE=4 SV=1
MM1 pKa = 7.48FSLSDD6 pKa = 3.64FLSEE10 pKa = 3.53IGNVIRR16 pKa = 11.84EE17 pKa = 4.26VEE19 pKa = 4.09DD20 pKa = 3.61KK21 pKa = 11.13DD22 pKa = 3.54NKK24 pKa = 10.46EE25 pKa = 4.0YY26 pKa = 10.71EE27 pKa = 4.06DD28 pKa = 4.33QIRR31 pKa = 11.84EE32 pKa = 4.04IQNLHH37 pKa = 6.47RR38 pKa = 11.84DD39 pKa = 3.69MNHH42 pKa = 5.32VEE44 pKa = 4.39LKK46 pKa = 10.07VSEE49 pKa = 4.59NIKK52 pKa = 10.08KK53 pKa = 10.23ACPEE57 pKa = 3.95LTEE60 pKa = 4.34TEE62 pKa = 4.5ANLSSDD68 pKa = 3.31NTIIDD73 pKa = 4.28LKK75 pKa = 11.2DD76 pKa = 3.6DD77 pKa = 4.05ALTEE81 pKa = 4.1YY82 pKa = 11.01KK83 pKa = 10.55EE84 pKa = 4.25IVWKK88 pKa = 10.25IDD90 pKa = 3.1NDD92 pKa = 3.65EE93 pKa = 4.1SVEE96 pKa = 4.03MSKK99 pKa = 8.57NTIKK103 pKa = 10.92EE104 pKa = 4.07MVFKK108 pKa = 11.23NKK110 pKa = 9.85DD111 pKa = 3.3DD112 pKa = 3.87EE113 pKa = 5.52LIISSNLDD121 pKa = 3.05LDD123 pKa = 4.31QNVCNGNSDD132 pKa = 4.06CDD134 pKa = 3.58VSNAKK139 pKa = 10.24KK140 pKa = 10.63SEE142 pKa = 3.86NGEE145 pKa = 4.17NFLEE149 pKa = 4.48THH151 pKa = 6.5TDD153 pKa = 3.83MIPPINTSDD162 pKa = 3.65CNHH165 pKa = 6.42INEE168 pKa = 4.48ILDD171 pKa = 3.46NGTYY175 pKa = 10.03EE176 pKa = 4.66RR177 pKa = 11.84IAIFSNPITINLDD190 pKa = 3.43DD191 pKa = 4.05GRR193 pKa = 11.84KK194 pKa = 9.28LIKK197 pKa = 9.64VTIADD202 pKa = 3.75EE203 pKa = 4.49NIKK206 pKa = 10.68KK207 pKa = 10.31KK208 pKa = 10.92VEE210 pKa = 4.14SSVKK214 pKa = 10.23EE215 pKa = 3.82ITCIPIYY222 pKa = 10.59KK223 pKa = 9.9LGNRR227 pKa = 11.84GDD229 pKa = 3.76YY230 pKa = 11.01DD231 pKa = 5.03SIISNCMSMDD241 pKa = 3.48GVIKK245 pKa = 10.73KK246 pKa = 8.3LTGTLIIYY254 pKa = 10.07CSLDD258 pKa = 4.11DD259 pKa = 3.84VFSWLILKK267 pKa = 8.55SVPNDD272 pKa = 3.14

Molecular weight: 30.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O89162|O89162_9VIRU 18.9K protein OS=Rice grassy stunt tenuivirus OX=66266 GN=pV1 PE=4 SV=1
MM1 pKa = 7.89ALLQKK6 pKa = 10.59LGSSKK11 pKa = 10.45VSSKK15 pKa = 10.69RR16 pKa = 11.84MSPAMIPLDD25 pKa = 5.03SINQDD30 pKa = 3.14LVDD33 pKa = 4.12PQQEE37 pKa = 3.68KK38 pKa = 10.33DD39 pKa = 3.35AKK41 pKa = 10.28NKK43 pKa = 10.45KK44 pKa = 8.77EE45 pKa = 4.11GKK47 pKa = 10.16KK48 pKa = 9.96KK49 pKa = 10.7DD50 pKa = 4.13LDD52 pKa = 3.65VSMDD56 pKa = 3.94PLTGKK61 pKa = 10.57LPLGKK66 pKa = 9.91KK67 pKa = 9.49KK68 pKa = 10.4QVDD71 pKa = 3.58TGGIAYY77 pKa = 10.21LEE79 pKa = 4.23NALMQLDD86 pKa = 3.83LHH88 pKa = 6.91DD89 pKa = 4.87FSFDD93 pKa = 3.7SIRR96 pKa = 11.84PRR98 pKa = 11.84TKK100 pKa = 8.32TFHH103 pKa = 6.77MKK105 pKa = 9.86RR106 pKa = 11.84QHH108 pKa = 6.16FKK110 pKa = 10.33ISTVNSRR117 pKa = 11.84FRR119 pKa = 11.84LDD121 pKa = 3.38VEE123 pKa = 4.29KK124 pKa = 10.27TGLFSKK130 pKa = 8.16TLKK133 pKa = 10.28YY134 pKa = 10.96SRR136 pKa = 11.84ICTLCLAFLGIKK148 pKa = 9.83NRR150 pKa = 11.84AQGTISFTFRR160 pKa = 11.84DD161 pKa = 3.68LSYY164 pKa = 11.02LSEE167 pKa = 4.15NDD169 pKa = 3.47QIDD172 pKa = 4.08FKK174 pKa = 11.44VKK176 pKa = 10.34NRR178 pKa = 11.84ISKK181 pKa = 9.99SFSAIASFPAPIFNDD196 pKa = 3.3DD197 pKa = 4.59LGNLICDD204 pKa = 4.22FEE206 pKa = 4.69IEE208 pKa = 4.21NASVNGVVIGDD219 pKa = 4.06LLVLLGIEE227 pKa = 4.21QSDD230 pKa = 4.08LPVCYY235 pKa = 9.66EE236 pKa = 3.8PQKK239 pKa = 11.46AKK241 pKa = 10.38IFEE244 pKa = 4.32YY245 pKa = 10.78KK246 pKa = 10.35PLTEE250 pKa = 4.7KK251 pKa = 10.96GLNKK255 pKa = 9.87ISNFAGYY262 pKa = 10.15VDD264 pKa = 4.49NVLKK268 pKa = 10.73AAINHH273 pKa = 6.57RR274 pKa = 11.84EE275 pKa = 4.04GEE277 pKa = 4.07DD278 pKa = 3.42DD279 pKa = 3.97GFSTEE284 pKa = 3.92GLGVLVHH291 pKa = 6.14PRR293 pKa = 11.84VKK295 pKa = 10.64QIDD298 pKa = 3.38NSIPIKK304 pKa = 10.61SLEE307 pKa = 4.07NKK309 pKa = 7.42PQKK312 pKa = 10.33MLMRR316 pKa = 11.84DD317 pKa = 3.43GSYY320 pKa = 11.28LDD322 pKa = 3.81VNPP325 pKa = 4.65

Molecular weight: 36.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

6290

165

2925

524.2

60.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.529 ± 0.515

2.003 ± 0.331

6.979 ± 0.438

6.439 ± 0.517

4.165 ± 0.296

4.785 ± 0.254

2.226 ± 0.129

7.107 ± 0.622

7.393 ± 0.353

9.905 ± 0.324

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.275 ± 0.328

5.517 ± 0.443

3.084 ± 0.217

3.18 ± 0.28

4.992 ± 0.291

8.378 ± 0.378

5.501 ± 0.387

6.216 ± 0.512

1.065 ± 0.095

4.261 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski