Tobacco leaf curl Cuba virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I9ZKR0|A0A1I9ZKR0_9GEMI Movement protein BC1 OS=Tobacco leaf curl Cuba virus OX=336987 GN=BC1 PE=3 SV=1
MM1 pKa = 7.34PRR3 pKa = 11.84KK4 pKa = 9.88GSSIANAKK12 pKa = 10.35NYY14 pKa = 9.83FLTYY18 pKa = 8.4PQCSLTKK25 pKa = 10.58EE26 pKa = 4.04EE27 pKa = 5.55ALSQLKK33 pKa = 10.26KK34 pKa = 11.02LNTPVNKK41 pKa = 10.08KK42 pKa = 9.89FIKK45 pKa = 9.7ICRR48 pKa = 11.84EE49 pKa = 3.55LHH51 pKa = 6.01QDD53 pKa = 4.0GKK55 pKa = 9.97PHH57 pKa = 6.22LHH59 pKa = 6.21VLIQFEE65 pKa = 4.49GKK67 pKa = 10.13YY68 pKa = 10.33NCTNNRR74 pKa = 11.84FFDD77 pKa = 3.9LVSPSRR83 pKa = 11.84SAHH86 pKa = 4.9FHH88 pKa = 6.45PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.7KK106 pKa = 11.33DD107 pKa = 3.49GDD109 pKa = 3.61TLEE112 pKa = 4.09WGKK115 pKa = 9.64FQIDD119 pKa = 3.2GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQTTNDD132 pKa = 3.94TYY134 pKa = 11.88AKK136 pKa = 10.31ALNASSAEE144 pKa = 4.0EE145 pKa = 3.51ALQIIKK151 pKa = 10.28EE152 pKa = 4.13EE153 pKa = 4.13QPQHH157 pKa = 6.73FFLQHH162 pKa = 6.07HH163 pKa = 6.02NLVANAHH170 pKa = 6.8RR171 pKa = 11.84IFQKK175 pKa = 10.76APEE178 pKa = 4.11PWVPPFQLSSFTNVPEE194 pKa = 4.75EE195 pKa = 4.06MQDD198 pKa = 2.95WADD201 pKa = 3.6EE202 pKa = 4.26YY203 pKa = 11.08FGRR206 pKa = 11.84GSAARR211 pKa = 11.84PEE213 pKa = 4.25DD214 pKa = 3.41QQ215 pKa = 4.52

Molecular weight: 24.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I9ZKP1|A0A1I9ZKP1_9GEMI Transcriptional activator protein OS=Tobacco leaf curl Cuba virus OX=336987 GN=AC2 PE=3 SV=1
MM1 pKa = 7.2YY2 pKa = 9.7PLRR5 pKa = 11.84NKK7 pKa = 9.92RR8 pKa = 11.84GSYY11 pKa = 8.03FTARR15 pKa = 11.84RR16 pKa = 11.84YY17 pKa = 9.85YY18 pKa = 9.9PRR20 pKa = 11.84NTFSRR25 pKa = 11.84RR26 pKa = 11.84PSTSKK31 pKa = 10.62KK32 pKa = 9.34QDD34 pKa = 2.89GKK36 pKa = 10.84RR37 pKa = 11.84RR38 pKa = 11.84AVNTNKK44 pKa = 9.93TNDD47 pKa = 3.81EE48 pKa = 4.39PKK50 pKa = 9.89MSAQRR55 pKa = 11.84IHH57 pKa = 6.58EE58 pKa = 4.15NQYY61 pKa = 11.02GPDD64 pKa = 3.08FVMAHH69 pKa = 5.33NTAISTFISYY79 pKa = 9.73PGLGSTLPNRR89 pKa = 11.84SRR91 pKa = 11.84SYY93 pKa = 10.98IKK95 pKa = 10.57LKK97 pKa = 8.43QLRR100 pKa = 11.84FKK102 pKa = 10.43GTVKK106 pKa = 10.11IEE108 pKa = 3.98RR109 pKa = 11.84VQSDD113 pKa = 3.91LNMDD117 pKa = 3.77GSTPKK122 pKa = 10.35VEE124 pKa = 4.64GVFSLVVVVDD134 pKa = 4.97RR135 pKa = 11.84KK136 pKa = 9.68PHH138 pKa = 5.5VGPSGCLQSFDD149 pKa = 4.11EE150 pKa = 4.66LFGARR155 pKa = 11.84IHH157 pKa = 6.19SHH159 pKa = 6.03GNLNVTSALKK169 pKa = 9.41DD170 pKa = 3.22RR171 pKa = 11.84YY172 pKa = 10.14YY173 pKa = 10.27IRR175 pKa = 11.84HH176 pKa = 4.2VCKK179 pKa = 10.24RR180 pKa = 11.84VFSVEE185 pKa = 3.95KK186 pKa = 9.13DD187 pKa = 3.12TLMVDD192 pKa = 3.36VEE194 pKa = 4.49GFIPLSNKK202 pKa = 9.46RR203 pKa = 11.84FNCWSTFKK211 pKa = 11.21DD212 pKa = 4.26LDD214 pKa = 3.81RR215 pKa = 11.84DD216 pKa = 3.98SCKK219 pKa = 10.63GVYY222 pKa = 10.46DD223 pKa = 4.52NISKK227 pKa = 10.38NALLVYY233 pKa = 8.04YY234 pKa = 9.89CWMSDD239 pKa = 3.29TMSTASTFVSFDD251 pKa = 3.39LDD253 pKa = 3.91YY254 pKa = 11.72VGG256 pKa = 5.46

Molecular weight: 29.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1361

85

293

194.4

22.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.511 ± 0.567

1.91 ± 0.284

5.143 ± 0.516

4.555 ± 0.625

4.115 ± 0.529

5.07 ± 0.381

3.894 ± 0.45

5.731 ± 0.577

5.584 ± 0.668

6.686 ± 0.82

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.572 ± 0.51

5.584 ± 0.605

4.996 ± 0.677

4.115 ± 0.684

7.788 ± 0.79

8.964 ± 1.02

6.025 ± 0.646

5.952 ± 0.927

1.396 ± 0.147

4.409 ± 0.843
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski