Proteus phage PM87

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PRB1|A0A2H4PRB1_9CAUD Putative DNA modification protein Mom-like protein OS=Proteus phage PM87 OX=2048007 PE=4 SV=1
MM1 pKa = 7.87AEE3 pKa = 3.8LAYY6 pKa = 9.35TKK8 pKa = 10.86ASIFQSIVDD17 pKa = 4.18DD18 pKa = 4.38VNTGLSKK25 pKa = 9.96EE26 pKa = 4.39TSLDD30 pKa = 3.31TRR32 pKa = 11.84GDD34 pKa = 3.58FEE36 pKa = 4.22NQLRR40 pKa = 11.84DD41 pKa = 3.57LFEE44 pKa = 5.04EE45 pKa = 4.29NNLMNAPEE53 pKa = 5.22CISVDD58 pKa = 3.34HH59 pKa = 6.9YY60 pKa = 11.41GDD62 pKa = 2.78AWEE65 pKa = 4.69IVQSSEE71 pKa = 3.66FDD73 pKa = 3.62SVEE76 pKa = 4.18LEE78 pKa = 4.23TPLDD82 pKa = 3.83FSGCEE87 pKa = 3.7SSLNCLIIEE96 pKa = 4.25ANEE99 pKa = 4.47LIQASWRR106 pKa = 11.84EE107 pKa = 3.85QVEE110 pKa = 4.23AYY112 pKa = 9.77IEE114 pKa = 4.01EE115 pKa = 4.16LAEE118 pKa = 4.03ALEE121 pKa = 4.34EE122 pKa = 4.18VIEE125 pKa = 4.51GCEE128 pKa = 3.73EE129 pKa = 4.07FGTIDD134 pKa = 3.86EE135 pKa = 4.6LVITDD140 pKa = 4.03GCRR143 pKa = 11.84FGHH146 pKa = 6.32IPHH149 pKa = 6.68LRR151 pKa = 11.84EE152 pKa = 3.31FDD154 pKa = 3.64AGDD157 pKa = 3.68FSVCMWAPNEE167 pKa = 4.12AGEE170 pKa = 4.69SEE172 pKa = 3.88IYY174 pKa = 10.74YY175 pKa = 10.17NFRR178 pKa = 11.84GLEE181 pKa = 3.89LRR183 pKa = 11.84GVVYY187 pKa = 10.63HH188 pKa = 6.78GG189 pKa = 3.79

Molecular weight: 21.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PR94|A0A2H4PR94_9CAUD Uncharacterized protein OS=Proteus phage PM87 OX=2048007 PE=4 SV=1
MM1 pKa = 7.48FFLLEE6 pKa = 3.91HH7 pKa = 7.01LATEE11 pKa = 4.04RR12 pKa = 11.84AIIHH16 pKa = 5.43KK17 pKa = 9.6HH18 pKa = 4.47RR19 pKa = 11.84KK20 pKa = 8.43IRR22 pKa = 11.84LSYY25 pKa = 9.94FFIEE29 pKa = 4.46RR30 pKa = 11.84PVFPPVLVVRR40 pKa = 11.84EE41 pKa = 3.96EE42 pKa = 3.96LKK44 pKa = 10.94EE45 pKa = 3.78VGRR48 pKa = 11.84CHH50 pKa = 6.99RR51 pKa = 11.84FVSHH55 pKa = 7.08DD56 pKa = 3.43VRR58 pKa = 11.84FSFPPHH64 pKa = 6.04RR65 pKa = 11.84VYY67 pKa = 11.25SSAFRR72 pKa = 3.97

Molecular weight: 8.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

17019

54

1445

298.6

33.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.562 ± 0.523

0.899 ± 0.118

6.51 ± 0.246

6.998 ± 0.321

4.066 ± 0.207

7.139 ± 0.27

1.551 ± 0.141

5.999 ± 0.177

6.24 ± 0.379

7.55 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.124

4.707 ± 0.173

4.577 ± 0.396

3.173 ± 0.226

5.717 ± 0.205

6.687 ± 0.36

5.993 ± 0.255

7.133 ± 0.336

1.434 ± 0.147

3.367 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski