Bean necrotic mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Tospoviridae; Orthotospovirus; Bean necrotic mosaic orthotospovirus

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|I3PCT2|I3PCT2_9VIRU Non-structural movement protein OS=Bean necrotic mosaic virus OX=1033976 GN=NSm PE=4 SV=1
MM1 pKa = 7.61GFRR4 pKa = 11.84EE5 pKa = 4.3VFVIICLFCLNLVFLDD21 pKa = 4.47FSINAHH27 pKa = 4.93TRR29 pKa = 11.84GDD31 pKa = 3.76HH32 pKa = 6.28EE33 pKa = 4.68LSQEE37 pKa = 3.74ISFEE41 pKa = 4.25DD42 pKa = 3.88LSKK45 pKa = 11.29DD46 pKa = 3.39AVEE49 pKa = 4.34SATLLGVDD57 pKa = 4.74GKK59 pKa = 10.88FKK61 pKa = 10.88SGPLLDD67 pKa = 5.34KK68 pKa = 10.9PDD70 pKa = 4.83IDD72 pKa = 5.03DD73 pKa = 3.78QMIPNRR79 pKa = 11.84GKK81 pKa = 8.94RR82 pKa = 11.84TEE84 pKa = 4.39PSSSSPPSSEE94 pKa = 3.6QLTCKK99 pKa = 10.45RR100 pKa = 11.84INSNNCKK107 pKa = 9.82IVGKK111 pKa = 8.87TSFNFYY117 pKa = 10.53YY118 pKa = 10.32QISDD122 pKa = 3.55NEE124 pKa = 4.55TTVSCLSSEE133 pKa = 4.18SQDD136 pKa = 3.53YY137 pKa = 10.71KK138 pKa = 11.16SCNPDD143 pKa = 2.72AMQEE147 pKa = 4.06FLSADD152 pKa = 3.6VLPITTMLNKK162 pKa = 9.98RR163 pKa = 11.84VLSVGSKK170 pKa = 10.26YY171 pKa = 10.66FILNSADD178 pKa = 3.32PSSYY182 pKa = 9.98PVQDD186 pKa = 3.54HH187 pKa = 6.69GSVQLGLGNIYY198 pKa = 10.21NVRR201 pKa = 11.84LTGGCNITKK210 pKa = 10.05VSLANPFYY218 pKa = 10.01ITLSSTAKK226 pKa = 10.34KK227 pKa = 10.32CLCCKK232 pKa = 10.41KK233 pKa = 10.31IGSEE237 pKa = 4.23EE238 pKa = 4.05VNLMKK243 pKa = 10.59FSSEE247 pKa = 3.75KK248 pKa = 10.19TIEE251 pKa = 4.0LSEE254 pKa = 4.74DD255 pKa = 3.71SLDD258 pKa = 3.76GTHH261 pKa = 6.92VLLCGDD267 pKa = 4.22RR268 pKa = 11.84VSEE271 pKa = 4.56IPKK274 pKa = 9.62IDD276 pKa = 3.3INKK279 pKa = 9.51RR280 pKa = 11.84NCLVKK285 pKa = 10.93YY286 pKa = 9.93KK287 pKa = 10.54GKK289 pKa = 10.46RR290 pKa = 11.84YY291 pKa = 8.06QQSACVHH298 pKa = 5.94FNLLRR303 pKa = 11.84YY304 pKa = 9.71LIVSIILFFPVSWFLNKK321 pKa = 9.54TKK323 pKa = 10.81EE324 pKa = 5.11SMFLWYY330 pKa = 9.95DD331 pKa = 2.88ILGIVLYY338 pKa = 9.88PLLYY342 pKa = 10.06ALNWIWQYY350 pKa = 11.16FPLKK354 pKa = 10.32CSCCGNFSLITHH366 pKa = 6.83KK367 pKa = 10.76CYY369 pKa = 10.44EE370 pKa = 4.13KK371 pKa = 10.77CVCNLSKK378 pKa = 10.75AKK380 pKa = 10.17KK381 pKa = 8.88EE382 pKa = 3.96HH383 pKa = 7.27AEE385 pKa = 3.98TCQILTGKK393 pKa = 8.55IFKK396 pKa = 9.59MKK398 pKa = 9.57EE399 pKa = 3.49VKK401 pKa = 10.27AEE403 pKa = 3.8KK404 pKa = 10.35RR405 pKa = 11.84EE406 pKa = 4.31AITPDD411 pKa = 3.72EE412 pKa = 4.13IVSLNSEE419 pKa = 4.09QEE421 pKa = 3.94KK422 pKa = 10.51PPVIKK427 pKa = 10.41SSRR430 pKa = 11.84FQTFQTIINTKK441 pKa = 9.71ISSEE445 pKa = 4.11TLLLITKK452 pKa = 9.77ILLGFLIFSQIPKK465 pKa = 8.83TMAMSNEE472 pKa = 3.56QALCVNYY479 pKa = 10.1CRR481 pKa = 11.84AQIGCSKK488 pKa = 10.09FLWKK492 pKa = 10.72NEE494 pKa = 3.85EE495 pKa = 4.09TCISRR500 pKa = 11.84KK501 pKa = 8.59DD502 pKa = 3.65LKK504 pKa = 10.7CNCVIGKK511 pKa = 8.41EE512 pKa = 4.25LISEE516 pKa = 3.97EE517 pKa = 4.33AYY519 pKa = 10.12INGTRR524 pKa = 11.84YY525 pKa = 9.55AVSMPNTCMDD535 pKa = 3.84DD536 pKa = 3.58TCDD539 pKa = 3.49VAEE542 pKa = 4.76GDD544 pKa = 3.87VEE546 pKa = 4.13NLIVCRR552 pKa = 11.84LGCKK556 pKa = 9.8KK557 pKa = 10.28LRR559 pKa = 11.84SLKK562 pKa = 8.65TAKK565 pKa = 9.98LSKK568 pKa = 9.48RR569 pKa = 11.84TFSTGIFSQSLSMNQIYY586 pKa = 8.05STLRR590 pKa = 11.84MQDD593 pKa = 3.19GYY595 pKa = 10.71IDD597 pKa = 3.81SLEE600 pKa = 4.22ALSTLGDD607 pKa = 3.77PPSDD611 pKa = 3.07KK612 pKa = 10.71VVQIDD617 pKa = 3.59DD618 pKa = 5.51DD619 pKa = 4.02IPEE622 pKa = 5.29GILPRR627 pKa = 11.84QSFVYY632 pKa = 10.48SSVVDD637 pKa = 3.84GKK639 pKa = 10.96YY640 pKa = 9.88RR641 pKa = 11.84YY642 pKa = 10.12LMSFDD647 pKa = 5.65AIQSSGYY654 pKa = 8.56VYY656 pKa = 10.95SMKK659 pKa = 9.71MLKK662 pKa = 8.59WHH664 pKa = 7.13PPQEE668 pKa = 3.85LVVYY672 pKa = 9.66VKK674 pKa = 10.52EE675 pKa = 4.22AGVTYY680 pKa = 9.21TIKK683 pKa = 10.48PLYY686 pKa = 8.21HH687 pKa = 6.44TAPISSTHH695 pKa = 3.84THH697 pKa = 6.18VYY699 pKa = 6.48TTCTGNCEE707 pKa = 4.41TCRR710 pKa = 11.84KK711 pKa = 7.53EE712 pKa = 4.37HH713 pKa = 6.76PLSGYY718 pKa = 9.12QDD720 pKa = 3.78YY721 pKa = 11.11CISPTSNWGCEE732 pKa = 4.03EE733 pKa = 4.63LGCLAIGEE741 pKa = 4.66GSTCGYY747 pKa = 9.08CRR749 pKa = 11.84NVYY752 pKa = 9.95DD753 pKa = 4.19LSKK756 pKa = 9.92MYY758 pKa = 10.51SINQVLSSHH767 pKa = 5.38VTVKK771 pKa = 10.58VCFKK775 pKa = 10.39GFAGATCQTITDD787 pKa = 4.85EE788 pKa = 4.58IPYY791 pKa = 9.79QNSYY795 pKa = 9.12YY796 pKa = 10.34QISLDD801 pKa = 3.9ADD803 pKa = 3.8LHH805 pKa = 6.72NDD807 pKa = 3.56DD808 pKa = 5.42LGSGSRR814 pKa = 11.84IAIDD818 pKa = 3.64PNGLIMKK825 pKa = 9.71GNIANLKK832 pKa = 10.19DD833 pKa = 3.1SSTAFGHH840 pKa = 5.9PQLDD844 pKa = 3.63KK845 pKa = 10.87TGKK848 pKa = 10.32LLFGKK853 pKa = 10.51ANLDD857 pKa = 3.78LNDD860 pKa = 4.07FTWSCAVIGSKK871 pKa = 10.46HH872 pKa = 5.9VDD874 pKa = 3.02IKK876 pKa = 11.06KK877 pKa = 10.21CGYY880 pKa = 8.35DD881 pKa = 3.18TYY883 pKa = 10.7HH884 pKa = 6.8QYY886 pKa = 10.67IGLEE890 pKa = 4.46PISNHH895 pKa = 5.13YY896 pKa = 10.4ASEE899 pKa = 4.08ISDD902 pKa = 3.17EE903 pKa = 4.13KK904 pKa = 11.45LFVKK908 pKa = 10.41KK909 pKa = 10.34DD910 pKa = 3.81FKK912 pKa = 11.01VGKK915 pKa = 9.92INLIVDD921 pKa = 4.46LPSEE925 pKa = 4.35LFKK928 pKa = 11.3QPAKK932 pKa = 10.09KK933 pKa = 10.15PKK935 pKa = 9.4VSLINSEE942 pKa = 4.45CKK944 pKa = 10.48GCLSCGSGIKK954 pKa = 9.1CTLEE958 pKa = 3.87MTSDD962 pKa = 3.91SIFAGNINWEE972 pKa = 4.33HH973 pKa = 6.19CTSEE977 pKa = 4.11PEE979 pKa = 4.05YY980 pKa = 10.69IAMKK984 pKa = 10.11KK985 pKa = 9.89GSNIIKK991 pKa = 10.53VNMFCLKK998 pKa = 10.5NPSLTKK1004 pKa = 10.1MILEE1008 pKa = 4.27PSGDD1012 pKa = 3.52SDD1014 pKa = 4.67LSLEE1018 pKa = 4.14FEE1020 pKa = 4.31TRR1022 pKa = 11.84NVEE1025 pKa = 4.14IIDD1028 pKa = 3.82PEE1030 pKa = 4.43TIIDD1034 pKa = 3.98HH1035 pKa = 6.9NDD1037 pKa = 3.07DD1038 pKa = 3.8AFNEE1042 pKa = 4.22EE1043 pKa = 3.5VDD1045 pKa = 3.8YY1046 pKa = 11.77DD1047 pKa = 3.76NDD1049 pKa = 3.75TSFKK1053 pKa = 11.12SLWDD1057 pKa = 3.63YY1058 pKa = 11.37LKK1060 pKa = 11.28SPFNWIASFFGDD1072 pKa = 3.47FFEE1075 pKa = 4.37IVRR1078 pKa = 11.84VLLVLACCVFAAICLSKK1095 pKa = 10.74LFEE1098 pKa = 4.19ICRR1101 pKa = 11.84EE1102 pKa = 4.08YY1103 pKa = 11.12YY1104 pKa = 9.97KK1105 pKa = 10.29QEE1107 pKa = 3.98HH1108 pKa = 5.84YY1109 pKa = 10.39KK1110 pKa = 10.79KK1111 pKa = 10.41SVKK1114 pKa = 10.14SDD1116 pKa = 3.32LKK1118 pKa = 11.59DD1119 pKa = 3.23MDD1121 pKa = 4.4EE1122 pKa = 4.95EE1123 pKa = 5.15DD1124 pKa = 4.51PEE1126 pKa = 4.79DD1127 pKa = 4.63PEE1129 pKa = 7.43DD1130 pKa = 4.32ILGNLKK1136 pKa = 9.95NQITEE1141 pKa = 3.87DD1142 pKa = 3.03TLYY1145 pKa = 10.88RR1146 pKa = 11.84RR1147 pKa = 11.84NLKK1150 pKa = 9.62PKK1152 pKa = 9.93KK1153 pKa = 10.3SPFDD1157 pKa = 3.52SVMTT1161 pKa = 4.39

Molecular weight:
130.71 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|I3PCT0|I3PCT0_9VIRU Non-structural silencing protein OS=Bean necrotic mosaic virus OX=1033976 GN=NSs PE=4 SV=1
MM1 pKa = 7.62GSNALKK7 pKa = 10.91LNAEE11 pKa = 4.11NLKK14 pKa = 10.72KK15 pKa = 10.66LLLFNDD21 pKa = 3.6EE22 pKa = 4.85VEE24 pKa = 4.98FEE26 pKa = 4.17QQSTGFKK33 pKa = 10.56FVDD36 pKa = 4.12FCKK39 pKa = 10.43AHH41 pKa = 6.73EE42 pKa = 4.21KK43 pKa = 11.11DD44 pKa = 3.38KK45 pKa = 11.32FNPTSALTFLKK56 pKa = 10.23NRR58 pKa = 11.84KK59 pKa = 9.03AIYY62 pKa = 9.37SLCKK66 pKa = 10.41ASTFNYY72 pKa = 9.58GVYY75 pKa = 9.98EE76 pKa = 3.99IKK78 pKa = 10.68KK79 pKa = 9.76GDD81 pKa = 3.58KK82 pKa = 10.15ATDD85 pKa = 3.02TDD87 pKa = 4.46FTFKK91 pKa = 10.94RR92 pKa = 11.84LDD94 pKa = 2.99AFLRR98 pKa = 11.84VKK100 pKa = 10.82LLTHH104 pKa = 6.11CTKK107 pKa = 10.11IWDD110 pKa = 4.08GTDD113 pKa = 3.41PNAQTALCSEE123 pKa = 4.7LAKK126 pKa = 10.52IPIVKK131 pKa = 10.23AYY133 pKa = 9.87GLEE136 pKa = 4.08VSDD139 pKa = 4.84KK140 pKa = 8.6QKK142 pKa = 11.11CYY144 pKa = 10.27IAIVLGGNLSLLASLPGCEE163 pKa = 4.22VACFALAIFQDD174 pKa = 3.87LKK176 pKa = 11.25KK177 pKa = 10.61NEE179 pKa = 4.5LGIKK183 pKa = 10.03SDD185 pKa = 5.3FDD187 pKa = 3.91TKK189 pKa = 11.04DD190 pKa = 2.87QAGRR194 pKa = 11.84VAAVLDD200 pKa = 3.69AKK202 pKa = 10.92NFVFGEE208 pKa = 4.22PEE210 pKa = 3.69NEE212 pKa = 4.03KK213 pKa = 10.54LKK215 pKa = 10.92KK216 pKa = 9.43IAGILKK222 pKa = 10.03EE223 pKa = 4.03MTPTRR228 pKa = 11.84RR229 pKa = 11.84GHH231 pKa = 6.78AALTKK236 pKa = 10.07YY237 pKa = 10.63AEE239 pKa = 4.1QLSIISGVIGVTFEE253 pKa = 4.04MPGTSKK259 pKa = 10.94AKK261 pKa = 9.39EE262 pKa = 4.31SKK264 pKa = 10.18NHH266 pKa = 5.44GFMSS270 pKa = 4.01

Molecular weight:
29.83 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

5119

270

2932

1023.8

116.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.77 ± 0.726

2.559 ± 0.664

5.958 ± 0.247

6.896 ± 0.354

4.61 ± 0.415

4.884 ± 0.614

1.739 ± 0.15

7.111 ± 0.249

9.142 ± 0.449

9.807 ± 0.411

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.087 ± 0.554

5.997 ± 0.379

3.028 ± 0.38

2.774 ± 0.126

3.126 ± 0.431

9.631 ± 0.544

5.802 ± 0.417

5.45 ± 0.532

0.664 ± 0.165

3.966 ± 0.369

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski