Pseudomonas phage VW-6S

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GNF2|A0A2D1GNF2_9CAUD Tail tube protein OS=Pseudomonas phage VW-6S OX=2041487 GN=VW6S_40 PE=4 SV=1
MM1 pKa = 6.76TTPSSIDD8 pKa = 3.25SFEE11 pKa = 4.82AEE13 pKa = 3.43ISRR16 pKa = 11.84FYY18 pKa = 11.53ARR20 pKa = 11.84LADD23 pKa = 3.78SQVDD27 pKa = 3.61LGHH30 pKa = 6.53EE31 pKa = 4.27YY32 pKa = 9.56EE33 pKa = 4.59TVLLANISDD42 pKa = 4.43LYY44 pKa = 11.27EE45 pKa = 3.94EE46 pKa = 4.51

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GNN8|A0A2D1GNN8_9CAUD Defense against restriction protein A OS=Pseudomonas phage VW-6S OX=2041487 GN=VW6S_23 PE=4 SV=1
MM1 pKa = 7.38AARR4 pKa = 11.84RR5 pKa = 11.84SRR7 pKa = 11.84MSGDD11 pKa = 3.03FKK13 pKa = 11.12LRR15 pKa = 11.84RR16 pKa = 11.84TLRR19 pKa = 11.84TLHH22 pKa = 6.09QSMDD26 pKa = 3.54NEE28 pKa = 4.37LAPVMRR34 pKa = 11.84DD35 pKa = 2.92SAEE38 pKa = 4.54RR39 pKa = 11.84ILSTMKK45 pKa = 10.8NLIPKK50 pKa = 8.54DD51 pKa = 3.37TGAAAAALTVFVSQSGLDD69 pKa = 3.44AQIGIRR75 pKa = 11.84GKK77 pKa = 10.49KK78 pKa = 7.95NKK80 pKa = 9.15QRR82 pKa = 11.84FFYY85 pKa = 10.83LRR87 pKa = 11.84FVEE90 pKa = 4.7YY91 pKa = 8.86GTKK94 pKa = 10.43GYY96 pKa = 8.78TGNKK100 pKa = 7.92RR101 pKa = 11.84AGGRR105 pKa = 11.84TRR107 pKa = 11.84RR108 pKa = 11.84PTNKK112 pKa = 9.71ADD114 pKa = 3.12GSNFFGKK121 pKa = 10.74YY122 pKa = 8.68PDD124 pKa = 3.27IPARR128 pKa = 11.84PSHH131 pKa = 6.4PWLRR135 pKa = 11.84PAKK138 pKa = 9.62DD139 pKa = 3.26VNRR142 pKa = 11.84EE143 pKa = 3.78YY144 pKa = 11.19VIASIEE150 pKa = 3.88AAIARR155 pKa = 11.84TLSKK159 pKa = 10.65ASKK162 pKa = 10.58GLTNGG167 pKa = 3.39

Molecular weight: 18.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11431

46

1209

219.8

24.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.206 ± 0.807

0.936 ± 0.159

5.931 ± 0.214

5.756 ± 0.248

3.324 ± 0.205

8.319 ± 0.275

1.767 ± 0.234

4.706 ± 0.234

5.433 ± 0.303

8.696 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.799 ± 0.16

3.797 ± 0.203

4.427 ± 0.327

4.339 ± 0.277

5.651 ± 0.278

6.019 ± 0.296

6.124 ± 0.277

6.526 ± 0.35

1.68 ± 0.154

2.563 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski