Amino acid dipepetide frequency for Sphaeramia orbicularis (orbiculate cardinalfish)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.65AlaAla: 5.65 ± 0.022	1.271AlaCys: 1.271 ± 0.007	3.149AlaAsp: 3.149 ± 0.013	4.409AlaGlu: 4.409 ± 0.018	2.48AlaPhe: 2.48 ± 0.008	4.022AlaGly: 4.022 ± 0.016	1.486AlaHis: 1.486 ± 0.006	2.846AlaIle: 2.846 ± 0.011	3.382AlaLys: 3.382 ± 0.015	6.498AlaLeu: 6.498 ± 0.019	1.603AlaMet: 1.603 ± 0.008	2.19AlaAsn: 2.19 ± 0.008	3.086AlaPro: 3.086 ± 0.014	2.829AlaGln: 2.829 ± 0.012	2.967AlaArg: 2.967 ± 0.01	4.887AlaSer: 4.887 ± 0.014	3.302AlaThr: 3.302 ± 0.01	4.892AlaVal: 4.892 ± 0.014	0.659AlaTrp: 0.659 ± 0.005	1.622AlaTyr: 1.622 ± 0.008	0.0AlaXaa: 0.0 ± 0.0
Cys	1.19CysAla: 1.19 ± 0.008	0.645CysCys: 0.645 ± 0.006	1.265CysAsp: 1.265 ± 0.013	1.28CysGlu: 1.28 ± 0.01	1.011CysPhe: 1.011 ± 0.006	1.572CysGly: 1.572 ± 0.011	0.688CysHis: 0.688 ± 0.006	1.109CysIle: 1.109 ± 0.007	1.184CysLys: 1.184 ± 0.008	2.36CysLeu: 2.36 ± 0.014	0.498CysMet: 0.498 ± 0.004	0.872CysAsn: 0.872 ± 0.006	1.296CysPro: 1.296 ± 0.01	1.063CysGln: 1.063 ± 0.009	1.313CysArg: 1.313 ± 0.008	2.125CysSer: 2.125 ± 0.013	1.258CysThr: 1.258 ± 0.009	1.711CysVal: 1.711 ± 0.011	0.305CysTrp: 0.305 ± 0.003	0.649CysTyr: 0.649 ± 0.005	0.0CysXaa: 0.0 ± 0.0
Asp	3.02AspAla: 3.02 ± 0.012	1.19AspCys: 1.19 ± 0.008	3.169AspAsp: 3.169 ± 0.019	3.713AspGlu: 3.713 ± 0.014	2.24AspPhe: 2.24 ± 0.01	3.679AspGly: 3.679 ± 0.013	1.26AspHis: 1.26 ± 0.006	2.898AspIle: 2.898 ± 0.012	2.872AspLys: 2.872 ± 0.012	5.213AspLeu: 5.213 ± 0.016	1.363AspMet: 1.363 ± 0.006	2.056AspAsn: 2.056 ± 0.008	2.963AspPro: 2.963 ± 0.013	2.08AspGln: 2.08 ± 0.01	2.794AspArg: 2.794 ± 0.012	4.222AspSer: 4.222 ± 0.015	2.712AspThr: 2.712 ± 0.01	3.426AspVal: 3.426 ± 0.011	0.704AspTrp: 0.704 ± 0.005	1.657AspTyr: 1.657 ± 0.008	0.0AspXaa: 0.0 ± 0.0
Glu	4.615GluAla: 4.615 ± 0.019	1.317GluCys: 1.317 ± 0.011	4.27GluAsp: 4.27 ± 0.013	7.096GluGlu: 7.096 ± 0.032	2.078GluPhe: 2.078 ± 0.008	4.017GluGly: 4.017 ± 0.016	1.476GluHis: 1.476 ± 0.008	2.941GluIle: 2.941 ± 0.01	4.575GluLys: 4.575 ± 0.019	6.29GluLeu: 6.29 ± 0.021	1.746GluMet: 1.746 ± 0.008	2.755GluAsn: 2.755 ± 0.009	2.623GluPro: 2.623 ± 0.013	3.027GluGln: 3.027 ± 0.013	4.026GluArg: 4.026 ± 0.018	3.953GluSer: 3.953 ± 0.012	3.307GluThr: 3.307 ± 0.014	4.418GluVal: 4.418 ± 0.013	0.718GluTrp: 0.718 ± 0.005	1.734GluTyr: 1.734 ± 0.007	0.0GluXaa: 0.0 ± 0.0
Phe	2.024PheAla: 2.024 ± 0.008	1.073PheCys: 1.073 ± 0.007	1.93PheAsp: 1.93 ± 0.009	2.002PheGlu: 2.002 ± 0.009	1.896PhePhe: 1.896 ± 0.009	2.318PheGly: 2.318 ± 0.01	1.118PheHis: 1.118 ± 0.007	2.189PheIle: 2.189 ± 0.01	1.934PheLys: 1.934 ± 0.009	4.149PheLeu: 4.149 ± 0.015	0.899PheMet: 0.899 ± 0.005	1.625PheAsn: 1.625 ± 0.007	1.836PhePro: 1.836 ± 0.009	1.708PheGln: 1.708 ± 0.008	1.924PheArg: 1.924 ± 0.009	3.493PheSer: 3.493 ± 0.011	2.533PheThr: 2.533 ± 0.011	2.415PheVal: 2.415 ± 0.011	0.506PheTrp: 0.506 ± 0.004	1.369PheTyr: 1.369 ± 0.007	0.0PheXaa: 0.0 ± 0.0
Gly	3.839GlyAla: 3.839 ± 0.016	1.243GlyCys: 1.243 ± 0.008	3.197GlyAsp: 3.197 ± 0.012	3.892GlyGlu: 3.892 ± 0.014	2.495GlyPhe: 2.495 ± 0.011	5.053GlyGly: 5.053 ± 0.026	1.686GlyHis: 1.686 ± 0.008	2.742GlyIle: 2.742 ± 0.011	3.612GlyLys: 3.612 ± 0.013	5.539GlyLeu: 5.539 ± 0.026	1.482GlyMet: 1.482 ± 0.009	2.484GlyAsn: 2.484 ± 0.012	3.116GlyPro: 3.116 ± 0.024	2.716GlyGln: 2.716 ± 0.013	3.479GlyArg: 3.479 ± 0.012	5.322GlySer: 5.322 ± 0.024	3.421GlyThr: 3.421 ± 0.016	4.068GlyVal: 4.068 ± 0.014	0.785GlyTrp: 0.785 ± 0.005	1.927GlyTyr: 1.927 ± 0.01	0.0GlyXaa: 0.0 ± 0.0
His	1.305HisAla: 1.305 ± 0.007	0.812HisCys: 0.812 ± 0.007	1.034HisAsp: 1.034 ± 0.006	1.252HisGlu: 1.252 ± 0.006	1.169HisPhe: 1.169 ± 0.006	1.549HisGly: 1.549 ± 0.008	0.996HisHis: 0.996 ± 0.008	1.467HisIle: 1.467 ± 0.008	1.362HisLys: 1.362 ± 0.007	2.828HisLeu: 2.828 ± 0.012	0.716HisMet: 0.716 ± 0.005	1.069HisAsn: 1.069 ± 0.006	1.554HisPro: 1.554 ± 0.008	1.268HisGln: 1.268 ± 0.007	1.619HisArg: 1.619 ± 0.008	2.301HisSer: 2.301 ± 0.01	1.705HisThr: 1.705 ± 0.016	1.523HisVal: 1.523 ± 0.008	0.364HisTrp: 0.364 ± 0.003	0.913HisTyr: 0.913 ± 0.006	0.0HisXaa: 0.0 ± 0.0
Ile	2.635IleAla: 2.635 ± 0.01	1.173IleCys: 1.173 ± 0.006	2.329IleAsp: 2.329 ± 0.009	2.536IleGlu: 2.536 ± 0.01	2.028IlePhe: 2.028 ± 0.01	2.398IleGly: 2.398 ± 0.01	1.471IleHis: 1.471 ± 0.009	2.695IleIle: 2.695 ± 0.011	2.712IleLys: 2.712 ± 0.01	4.633IleLeu: 4.633 ± 0.015	1.171IleMet: 1.171 ± 0.006	2.149IleAsn: 2.149 ± 0.007	2.502IlePro: 2.502 ± 0.009	2.35IleGln: 2.35 ± 0.008	2.622IleArg: 2.622 ± 0.01	3.826IleSer: 3.826 ± 0.01	2.958IleThr: 2.958 ± 0.011	2.732IleVal: 2.732 ± 0.011	0.52IleTrp: 0.52 ± 0.004	1.588IleTyr: 1.588 ± 0.009	0.0IleXaa: 0.0 ± 0.0
Lys	3.763LysAla: 3.763 ± 0.016	1.075LysCys: 1.075 ± 0.007	3.361LysAsp: 3.361 ± 0.011	4.638LysGlu: 4.638 ± 0.02	1.774LysPhe: 1.774 ± 0.008	3.215LysGly: 3.215 ± 0.014	1.467LysHis: 1.467 ± 0.008	2.673LysIle: 2.673 ± 0.011	4.436LysLys: 4.436 ± 0.021	5.192LysLeu: 5.192 ± 0.016	1.512LysMet: 1.512 ± 0.007	2.423LysAsn: 2.423 ± 0.01	2.72LysPro: 2.72 ± 0.011	2.579LysGln: 2.579 ± 0.012	3.269LysArg: 3.269 ± 0.012	3.618LysSer: 3.618 ± 0.012	3.318LysThr: 3.318 ± 0.014	3.687LysVal: 3.687 ± 0.012	0.606LysTrp: 0.606 ± 0.005	1.638LysTyr: 1.638 ± 0.009	0.0LysXaa: 0.0 ± 0.0
Leu	5.981LeuAla: 5.981 ± 0.017	2.367LeuCys: 2.367 ± 0.009	5.134LeuAsp: 5.134 ± 0.016	6.445LeuGlu: 6.445 ± 0.024	3.799LeuPhe: 3.799 ± 0.016	5.148LeuGly: 5.148 ± 0.015	2.808LeuHis: 2.808 ± 0.011	4.159LeuIle: 4.159 ± 0.012	5.882LeuLys: 5.882 ± 0.017	10.274LeuLeu: 10.274 ± 0.027	2.273LeuMet: 2.273 ± 0.007	3.848LeuAsn: 3.848 ± 0.022	4.99LeuPro: 4.99 ± 0.014	5.476LeuGln: 5.476 ± 0.017	5.65LeuArg: 5.65 ± 0.018	8.113LeuSer: 8.113 ± 0.021	5.408LeuThr: 5.408 ± 0.013	5.743LeuVal: 5.743 ± 0.014	1.125LeuTrp: 1.125 ± 0.005	2.847LeuTyr: 2.847 ± 0.01	0.0LeuXaa: 0.0 ± 0.0
Met	1.891MetAla: 1.891 ± 0.007	0.533MetCys: 0.533 ± 0.004	1.514MetAsp: 1.514 ± 0.008	2.005MetGlu: 2.005 ± 0.009	0.962MetPhe: 0.962 ± 0.006	1.461MetGly: 1.461 ± 0.008	0.524MetHis: 0.524 ± 0.004	0.962MetIle: 0.962 ± 0.005	1.575MetLys: 1.575 ± 0.007	2.163MetLeu: 2.163 ± 0.01	0.723MetMet: 0.723 ± 0.005	1.001MetAsn: 1.001 ± 0.005	1.043MetPro: 1.043 ± 0.008	0.985MetGln: 0.985 ± 0.005	1.208MetArg: 1.208 ± 0.006	1.86MetSer: 1.86 ± 0.007	1.326MetThr: 1.326 ± 0.007	1.646MetVal: 1.646 ± 0.007	0.286MetTrp: 0.286 ± 0.003	0.704MetTyr: 0.704 ± 0.005	0.0MetXaa: 0.0 ± 0.0
Asn	2.209AsnAla: 2.209 ± 0.009	0.929AsnCys: 0.929 ± 0.006	1.806AsnAsp: 1.806 ± 0.008	2.159AsnGlu: 2.159 ± 0.01	1.504AsnPhe: 1.504 ± 0.007	2.741AsnGly: 2.741 ± 0.015	1.045AsnHis: 1.045 ± 0.006	2.361AsnIle: 2.361 ± 0.01	2.325AsnLys: 2.325 ± 0.009	3.799AsnLeu: 3.799 ± 0.011	1.106AsnMet: 1.106 ± 0.007	1.861AsnAsn: 1.861 ± 0.01	2.322AsnPro: 2.322 ± 0.012	1.779AsnGln: 1.779 ± 0.008	2.025AsnArg: 2.025 ± 0.009	3.128AsnSer: 3.128 ± 0.011	2.387AsnThr: 2.387 ± 0.01	2.435AsnVal: 2.435 ± 0.014	0.477AsnTrp: 0.477 ± 0.003	1.234AsnTyr: 1.234 ± 0.008	0.0AsnXaa: 0.0 ± 0.0
Pro	3.539ProAla: 3.539 ± 0.016	1.046ProCys: 1.046 ± 0.009	2.881ProAsp: 2.881 ± 0.01	3.635ProGlu: 3.635 ± 0.018	1.832ProPhe: 1.832 ± 0.009	3.804ProGly: 3.804 ± 0.026	1.469ProHis: 1.469 ± 0.008	1.907ProIle: 1.907 ± 0.008	2.41ProLys: 2.41 ± 0.011	4.698ProLeu: 4.698 ± 0.016	1.008ProMet: 1.008 ± 0.006	1.907ProAsn: 1.907 ± 0.008	4.816ProPro: 4.816 ± 0.034	2.445ProGln: 2.445 ± 0.011	2.505ProArg: 2.505 ± 0.01	4.881ProSer: 4.881 ± 0.024	2.985ProThr: 2.985 ± 0.022	3.675ProVal: 3.675 ± 0.015	0.573ProTrp: 0.573 ± 0.004	1.459ProTyr: 1.459 ± 0.008	0.0ProXaa: 0.0 ± 0.0
Gln	3.049GlnAla: 3.049 ± 0.013	1.108GlnCys: 1.108 ± 0.01	2.359GlnAsp: 2.359 ± 0.009	3.347GlnGlu: 3.347 ± 0.013	1.482GlnPhe: 1.482 ± 0.008	2.667GlnGly: 2.667 ± 0.012	1.285GlnHis: 1.285 ± 0.007	2.11GlnIle: 2.11 ± 0.009	2.596GlnLys: 2.596 ± 0.012	4.501GlnLeu: 4.501 ± 0.016	1.192GlnMet: 1.192 ± 0.007	1.791GlnAsn: 1.791 ± 0.008	2.292GlnPro: 2.292 ± 0.014	3.027GlnGln: 3.027 ± 0.025	2.996GlnArg: 2.996 ± 0.01	3.239GlnSer: 3.239 ± 0.012	2.638GlnThr: 2.638 ± 0.018	2.936GlnVal: 2.936 ± 0.012	0.59GlnTrp: 0.59 ± 0.005	1.359GlnTyr: 1.359 ± 0.007	0.0GlnXaa: 0.0 ± 0.0
Arg	3.2ArgAla: 3.2 ± 0.01	1.282ArgCys: 1.282 ± 0.01	2.913ArgAsp: 2.913 ± 0.011	3.634ArgGlu: 3.634 ± 0.015	2.049ArgPhe: 2.049 ± 0.009	3.266ArgGly: 3.266 ± 0.013	1.533ArgHis: 1.533 ± 0.007	2.546ArgIle: 2.546 ± 0.01	3.425ArgLys: 3.425 ± 0.011	5.37ArgLeu: 5.37 ± 0.015	1.328ArgMet: 1.328 ± 0.007	2.163ArgAsn: 2.163 ± 0.008	2.792ArgPro: 2.792 ± 0.012	2.629ArgGln: 2.629 ± 0.01	3.973ArgArg: 3.973 ± 0.015	4.172ArgSer: 4.172 ± 0.016	2.938ArgThr: 2.938 ± 0.012	3.319ArgVal: 3.319 ± 0.011	0.705ArgTrp: 0.705 ± 0.005	1.62ArgTyr: 1.62 ± 0.007	0.0ArgXaa: 0.0 ± 0.0
Ser	5.089SerAla: 5.089 ± 0.015	1.923SerCys: 1.923 ± 0.012	4.233SerAsp: 4.233 ± 0.014	4.554SerGlu: 4.554 ± 0.014	3.139SerPhe: 3.139 ± 0.011	5.329SerGly: 5.329 ± 0.019	2.123SerHis: 2.123 ± 0.008	3.406SerIle: 3.406 ± 0.011	3.836SerLys: 3.836 ± 0.011	7.895SerLeu: 7.895 ± 0.02	1.767SerMet: 1.767 ± 0.008	2.94SerAsn: 2.94 ± 0.01	5.1SerPro: 5.1 ± 0.022	3.554SerGln: 3.554 ± 0.019	4.115SerArg: 4.115 ± 0.015	8.888SerSer: 8.888 ± 0.042	4.653SerThr: 4.653 ± 0.026	5.452SerVal: 5.452 ± 0.016	0.973SerTrp: 0.973 ± 0.006	2.178SerTyr: 2.178 ± 0.009	0.0SerXaa: 0.0 ± 0.0
Thr	3.897ThrAla: 3.897 ± 0.012	1.444ThrCys: 1.444 ± 0.013	3.071ThrAsp: 3.071 ± 0.012	3.768ThrGlu: 3.768 ± 0.012	2.251ThrPhe: 2.251 ± 0.009	3.853ThrGly: 3.853 ± 0.019	1.535ThrHis: 1.535 ± 0.015	2.562ThrIle: 2.562 ± 0.01	2.717ThrLys: 2.717 ± 0.011	5.521ThrLeu: 5.521 ± 0.015	1.258ThrMet: 1.258 ± 0.006	2.031ThrAsn: 2.031 ± 0.011	3.49ThrPro: 3.49 ± 0.025	2.39ThrGln: 2.39 ± 0.01	2.463ThrArg: 2.463 ± 0.008	4.546ThrSer: 4.546 ± 0.024	3.429ThrThr: 3.429 ± 0.064	4.339ThrVal: 4.339 ± 0.016	0.692ThrTrp: 0.692 ± 0.006	1.562ThrTyr: 1.562 ± 0.008	0.0ThrXaa: 0.0 ± 0.0
Val	4.121ValAla: 4.121 ± 0.013	1.938ValCys: 1.938 ± 0.018	3.417ValAsp: 3.417 ± 0.012	4.099ValGlu: 4.099 ± 0.014	2.916ValPhe: 2.916 ± 0.01	3.588ValGly: 3.588 ± 0.019	1.717ValHis: 1.717 ± 0.007	3.187ValIle: 3.187 ± 0.011	3.729ValLys: 3.729 ± 0.014	6.477ValLeu: 6.477 ± 0.016	1.605ValMet: 1.605 ± 0.007	2.631ValAsn: 2.631 ± 0.01	3.174ValPro: 3.174 ± 0.011	2.851ValGln: 2.851 ± 0.012	3.409ValArg: 3.409 ± 0.014	5.236ValSer: 5.236 ± 0.015	4.011ValThr: 4.011 ± 0.013	4.566ValVal: 4.566 ± 0.016	0.822ValTrp: 0.822 ± 0.004	1.971ValTyr: 1.971 ± 0.009	0.0ValXaa: 0.0 ± 0.0
Trp	0.701TrpAla: 0.701 ± 0.005	0.265TrpCys: 0.265 ± 0.003	0.683TrpAsp: 0.683 ± 0.005	0.771TrpGlu: 0.771 ± 0.006	0.495TrpPhe: 0.495 ± 0.004	0.642TrpGly: 0.642 ± 0.004	0.274TrpHis: 0.274 ± 0.003	0.606TrpIle: 0.606 ± 0.005	0.768TrpLys: 0.768 ± 0.005	1.184TrpLeu: 1.184 ± 0.006	0.38TrpMet: 0.38 ± 0.003	0.539TrpAsn: 0.539 ± 0.004	0.427TrpPro: 0.427 ± 0.003	0.5TrpGln: 0.5 ± 0.004	0.736TrpArg: 0.736 ± 0.005	0.983TrpSer: 0.983 ± 0.007	0.765TrpThr: 0.765 ± 0.006	0.753TrpVal: 0.753 ± 0.005	0.22TrpTrp: 0.22 ± 0.003	0.364TrpTyr: 0.364 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.513TyrAla: 1.513 ± 0.007	0.763TyrCys: 0.763 ± 0.005	1.516TyrAsp: 1.516 ± 0.007	1.745TyrGlu: 1.745 ± 0.008	1.341TyrPhe: 1.341 ± 0.006	1.777TyrGly: 1.777 ± 0.009	0.843TyrHis: 0.843 ± 0.005	1.662TyrIle: 1.662 ± 0.008	1.633TyrLys: 1.633 ± 0.01	2.884TyrLeu: 2.884 ± 0.011	0.739TyrMet: 0.739 ± 0.005	1.296TyrAsn: 1.296 ± 0.006	1.334TyrPro: 1.334 ± 0.008	1.307TyrGln: 1.307 ± 0.005	1.764TyrArg: 1.764 ± 0.008	2.355TyrSer: 2.355 ± 0.01	1.766TyrThr: 1.766 ± 0.008	1.73TyrVal: 1.73 ± 0.009	0.423TyrTrp: 0.423 ± 0.005	1.079TyrTyr: 1.079 ± 0.007	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.005XaaXaa: 0.005 ± 0.002

Statistics based on 57747 proteins (36437828 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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