Archaeal BJ1 virus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 4.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0ZYN7|A0ZYN7_9CAUD Uncharacterized protein OS=Archaeal BJ1 virus OX=416419 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84ATILGLARR10 pKa = 11.84DD11 pKa = 4.67EE12 pKa = 4.5IADD15 pKa = 3.82PSIEE19 pKa = 4.08SHH21 pKa = 6.49IEE23 pKa = 3.59NATYY27 pKa = 9.14PAIDD31 pKa = 3.28VTAYY35 pKa = 9.71EE36 pKa = 4.41RR37 pKa = 11.84LDD39 pKa = 3.43GTTVYY44 pKa = 10.82AGDD47 pKa = 3.32AAGRR51 pKa = 11.84IDD53 pKa = 3.74VEE55 pKa = 4.04QEE57 pKa = 3.5EE58 pKa = 5.43IYY60 pKa = 10.63ISDD63 pKa = 3.17SSIIVEE69 pKa = 4.26PEE71 pKa = 3.55LVTDD75 pKa = 3.59YY76 pKa = 11.27SYY78 pKa = 11.72CEE80 pKa = 4.26WIADD84 pKa = 3.95LTEE87 pKa = 3.7GWLGVNRR94 pKa = 11.84SDD96 pKa = 5.2GDD98 pKa = 3.77WLFDD102 pKa = 3.28TLGAVKK108 pKa = 9.2GTLIHH113 pKa = 6.68RR114 pKa = 11.84AEE116 pKa = 3.93IDD118 pKa = 2.86IDD120 pKa = 3.96GFASYY125 pKa = 11.07LSDD128 pKa = 4.0YY129 pKa = 10.46PNASAWNTTQTRR141 pKa = 11.84SLDD144 pKa = 3.68ADD146 pKa = 4.05DD147 pKa = 5.35DD148 pKa = 4.67AEE150 pKa = 4.13EE151 pKa = 4.26STIKK155 pKa = 10.85YY156 pKa = 10.03HH157 pKa = 7.58DD158 pKa = 4.04AANLAEE164 pKa = 4.48TGGSGQTTMLGFEE177 pKa = 4.51YY178 pKa = 9.93FWNDD182 pKa = 2.85GYY184 pKa = 9.87TRR186 pKa = 11.84GVVSEE191 pKa = 4.24SGYY194 pKa = 10.0VALFEE199 pKa = 5.18GGDD202 pKa = 3.57RR203 pKa = 11.84PGTYY207 pKa = 10.2AAFINSEE214 pKa = 4.04ILPFASLPEE223 pKa = 4.36EE224 pKa = 4.15YY225 pKa = 10.05QGALTEE231 pKa = 4.22VVEE234 pKa = 4.4TDD236 pKa = 3.41DD237 pKa = 5.68HH238 pKa = 6.81GGQADD243 pKa = 4.08GEE245 pKa = 4.41

Molecular weight: 26.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0ZYQ4|A0ZYQ4_9CAUD Uncharacterized protein OS=Archaeal BJ1 virus OX=416419 PE=4 SV=1
MM1 pKa = 7.46SDD3 pKa = 2.98KK4 pKa = 10.31MALGGRR10 pKa = 11.84LKK12 pKa = 10.66GWAKK16 pKa = 10.33QGSGDD21 pKa = 4.3DD22 pKa = 3.86SPATGDD28 pKa = 4.45LMPRR32 pKa = 11.84TQGSVRR38 pKa = 11.84QGRR41 pKa = 11.84RR42 pKa = 11.84DD43 pKa = 3.42GRR45 pKa = 11.84PGPFCTSIHH54 pKa = 6.06HH55 pKa = 6.49AA56 pKa = 4.06

Molecular weight: 5.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13687

30

916

195.5

21.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.593 ± 0.29

0.738 ± 0.15

9.995 ± 0.284

9.14 ± 0.385

2.36 ± 0.149

8.307 ± 0.601

2.177 ± 0.216

3.975 ± 0.23

2.17 ± 0.243

7.511 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.556 ± 0.142

2.572 ± 0.214

4.734 ± 0.207

3.134 ± 0.184

7.05 ± 0.384

6.159 ± 0.288

6.758 ± 0.336

7.781 ± 0.33

1.425 ± 0.117

2.864 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski