Escherichia phage vB_EcoP_24B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sepvirinae; Traversvirus; Escherichia virus 24B

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G3CFI5|G3CFI5_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoP_24B OX=866553 GN=vb_24B_11c PE=4 SV=1
MM1 pKa = 7.47SNIKK5 pKa = 10.19KK6 pKa = 10.63YY7 pKa = 10.7IIDD10 pKa = 4.21YY11 pKa = 9.73DD12 pKa = 3.52WKK14 pKa = 10.79ASIEE18 pKa = 3.99IEE20 pKa = 3.8IDD22 pKa = 3.1HH23 pKa = 7.35DD24 pKa = 4.32VMTEE28 pKa = 3.99EE29 pKa = 5.57KK30 pKa = 10.35LHH32 pKa = 6.08QINNFWSDD40 pKa = 2.68SEE42 pKa = 4.22YY43 pKa = 11.07RR44 pKa = 11.84LNKK47 pKa = 9.52HH48 pKa = 5.94GSVLNAVLIMLAQHH62 pKa = 6.9ALLIAISSDD71 pKa = 2.92LNAYY75 pKa = 9.62GVVCEE80 pKa = 4.87FDD82 pKa = 3.48WNDD85 pKa = 3.26GNGQEE90 pKa = 5.08GWPSMDD96 pKa = 2.65GSEE99 pKa = 4.97GIRR102 pKa = 11.84ITDD105 pKa = 3.36IDD107 pKa = 3.67TSGIFDD113 pKa = 4.38SDD115 pKa = 3.93DD116 pKa = 3.52MTIKK120 pKa = 10.65AAA122 pKa = 3.9

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G3CFH2|G3CFH2_9CAUD DUF551 domain-containing protein OS=Escherichia phage vB_EcoP_24B OX=866553 GN=vb_24B_6c PE=4 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84RR3 pKa = 11.84GNRR6 pKa = 11.84HH7 pKa = 4.34TGCRR11 pKa = 11.84YY12 pKa = 8.08RR13 pKa = 11.84NRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84LTVANLQLARR28 pKa = 11.84SGIRR32 pKa = 11.84FVPIPITADD41 pKa = 3.23AEE43 pKa = 4.09LHH45 pKa = 6.09LFGEE49 pKa = 4.28ILSRR53 pKa = 11.84KK54 pKa = 9.52LDD56 pKa = 3.7EE57 pKa = 4.59LEE59 pKa = 4.25KK60 pKa = 10.79LVEE63 pKa = 4.24EE64 pKa = 5.24ADD66 pKa = 3.69TSPTVV71 pKa = 3.02

Molecular weight: 8.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

18903

22

2807

214.8

24.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.009 ± 0.431

1.508 ± 0.249

5.581 ± 0.204

6.517 ± 0.252

3.534 ± 0.223

7.385 ± 0.55

2.021 ± 0.201

5.11 ± 0.301

5.814 ± 0.186

7.798 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.777 ± 0.137

4.306 ± 0.181

4.576 ± 0.266

4.539 ± 0.348

6.925 ± 0.274

6.29 ± 0.214

5.56 ± 0.223

6.29 ± 0.265

1.524 ± 0.134

2.936 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski