Sodalis endosymbiont of Henestaris halophilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Bruguierivoracaceae; Sodalis; unclassified Sodalis

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 712 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2C8CWW4|A0A2C8CWW4_9GAMM Uncharacterized protein OS=Sodalis endosymbiont of Henestaris halophilus OX=1929246 GN=HBA_0196 PE=4 SV=1
MM1 pKa = 7.64FDD3 pKa = 3.14VLIYY7 pKa = 10.85LFEE10 pKa = 4.45TYY12 pKa = 9.07IHH14 pKa = 5.86AVEE17 pKa = 4.26MRR19 pKa = 11.84VDD21 pKa = 3.62QDD23 pKa = 4.01KK24 pKa = 9.64LTDD27 pKa = 3.71DD28 pKa = 3.84LTRR31 pKa = 11.84VGFHH35 pKa = 6.65QDD37 pKa = 3.36DD38 pKa = 4.38IYY40 pKa = 11.84NALNWLEE47 pKa = 4.41KK48 pKa = 10.75LADD51 pKa = 3.96LQDD54 pKa = 3.84GQGRR58 pKa = 11.84AVAFNADD65 pKa = 3.78PLAMRR70 pKa = 11.84IYY72 pKa = 10.58SEE74 pKa = 4.58EE75 pKa = 4.11EE76 pKa = 3.6NHH78 pKa = 7.33CLDD81 pKa = 3.5TCCRR85 pKa = 11.84GFLLFLEE92 pKa = 4.79QIQVLNLEE100 pKa = 4.22TRR102 pKa = 11.84EE103 pKa = 3.96MVIDD107 pKa = 3.81RR108 pKa = 11.84VMALDD113 pKa = 3.6AVEE116 pKa = 5.61FDD118 pKa = 5.21LEE120 pKa = 4.13DD121 pKa = 4.08LKK123 pKa = 11.14WVVLMVLFNIPGCEE137 pKa = 3.64NAYY140 pKa = 9.97QQMEE144 pKa = 4.16DD145 pKa = 3.7LVFEE149 pKa = 4.16EE150 pKa = 4.86HH151 pKa = 6.71VKK153 pKa = 10.51HH154 pKa = 6.39LHH156 pKa = 5.54

Molecular weight:
18.34 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2C8CXS0|A0A2C8CXS0_9GAMM 50S ribosomal protein L22 OS=Sodalis endosymbiont of Henestaris halophilus OX=1929246 GN=rplV PE=3 SV=1
MM1 pKa = 7.23NVIINLAPSEE11 pKa = 4.39WFQEE15 pKa = 4.06MRR17 pKa = 11.84VGRR20 pKa = 11.84YY21 pKa = 8.15SKK23 pKa = 10.95CDD25 pKa = 3.39IILGMTVKK33 pKa = 10.57LVLVARR39 pKa = 11.84ALINLTRR46 pKa = 11.84QLCISSAINAAHH58 pKa = 8.37RR59 pKa = 11.84IRR61 pKa = 11.84TSMSATSLL69 pKa = 3.56

Molecular weight:
7.69 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

712

0

712

200869

30

1406

282.1

31.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.061 ± 0.09

1.362 ± 0.03

4.991 ± 0.063

5.234 ± 0.082

3.782 ± 0.062

6.703 ± 0.082

2.502 ± 0.041

7.478 ± 0.08

5.209 ± 0.076

10.85 ± 0.118

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.872 ± 0.042

4.536 ± 0.059

3.805 ± 0.047

4.154 ± 0.062

5.919 ± 0.071

5.959 ± 0.06

5.281 ± 0.052

7.062 ± 0.077

1.126 ± 0.034

3.114 ± 0.055

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski