Ageratum yellow vein Hualian virus-[Taiwan:Hualian4:2000]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Ageratum yellow vein Hualian virus

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q09M35|Q09M35_9GEMI Transcriptional activator protein OS=Ageratum yellow vein Hualian virus-[Taiwan:Hualian4:2000] OX=437061 GN=AC2 PE=3 SV=1
MM1 pKa = 7.69APPRR5 pKa = 11.84QFKK8 pKa = 10.62INAKK12 pKa = 10.14NYY14 pKa = 8.77FLTYY18 pKa = 8.4PQCSLTKK25 pKa = 10.58EE26 pKa = 4.02EE27 pKa = 5.64ALSQLQNLNTPTNKK41 pKa = 10.12KK42 pKa = 9.69YY43 pKa = 10.84IKK45 pKa = 9.6ICKK48 pKa = 8.67EE49 pKa = 3.65LHH51 pKa = 6.4EE52 pKa = 5.71DD53 pKa = 4.28GSPHH57 pKa = 6.11LHH59 pKa = 6.16VLIQFEE65 pKa = 5.07GKK67 pKa = 8.95YY68 pKa = 9.35QCQNQRR74 pKa = 11.84FFDD77 pKa = 4.04LVSPTRR83 pKa = 11.84SAHH86 pKa = 4.73FHH88 pKa = 6.48PNIQRR93 pKa = 11.84AKK95 pKa = 9.77SSSDD99 pKa = 2.99VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.7KK106 pKa = 11.33DD107 pKa = 3.49GDD109 pKa = 3.67TLEE112 pKa = 4.1WGEE115 pKa = 3.95FQVDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQSANDD132 pKa = 4.11AYY134 pKa = 10.88AKK136 pKa = 10.41AINSGSKK143 pKa = 9.09TEE145 pKa = 3.95ALNVIRR151 pKa = 11.84EE152 pKa = 4.45LAPKK156 pKa = 10.23DD157 pKa = 3.59YY158 pKa = 11.19VLQFHH163 pKa = 6.73NLNSNLDD170 pKa = 3.99RR171 pKa = 11.84IFAPSLEE178 pKa = 4.28VFVCPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 3.95EE195 pKa = 4.04LEE197 pKa = 3.96EE198 pKa = 4.17WVSEE202 pKa = 4.24NVSDD206 pKa = 4.45AAARR210 pKa = 11.84PWRR213 pKa = 11.84PISIVIEE220 pKa = 4.23GDD222 pKa = 3.2SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.26YY287 pKa = 10.63GKK289 pKa = 9.49PIQIKK294 pKa = 10.42GGIPTIFLCNPGPTSSYY311 pKa = 11.03KK312 pKa = 10.58EE313 pKa = 3.93YY314 pKa = 11.1LNEE317 pKa = 4.81DD318 pKa = 3.49KK319 pKa = 11.27NSALKK324 pKa = 10.21EE325 pKa = 3.97WALKK329 pKa = 9.75NAEE332 pKa = 4.14FVTLNRR338 pKa = 11.84QLYY341 pKa = 10.38SGTNQSATQISQEE354 pKa = 4.2EE355 pKa = 4.26ASSQAEE361 pKa = 4.18SS362 pKa = 3.21

Molecular weight: 41.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q09M38|Q09M38_9GEMI Protein V2 OS=Ageratum yellow vein Hualian virus-[Taiwan:Hualian4:2000] OX=437061 GN=AV2 PE=3 SV=1
MM1 pKa = 7.73SKK3 pKa = 10.46RR4 pKa = 11.84PTDD7 pKa = 3.54IVISTPVSKK16 pKa = 10.55VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.27SPYY27 pKa = 8.86MSRR30 pKa = 11.84AAAPTVLVTNKK41 pKa = 9.37RR42 pKa = 11.84RR43 pKa = 11.84TWTNRR48 pKa = 11.84PMYY51 pKa = 9.79RR52 pKa = 11.84KK53 pKa = 8.55PRR55 pKa = 11.84LYY57 pKa = 10.82RR58 pKa = 11.84MYY60 pKa = 9.71RR61 pKa = 11.84TPDD64 pKa = 3.19VPKK67 pKa = 10.64GCEE70 pKa = 4.39GPCKK74 pKa = 9.35VQSYY78 pKa = 6.94EE79 pKa = 3.74QRR81 pKa = 11.84HH82 pKa = 6.38DD83 pKa = 3.16ISHH86 pKa = 5.75VGKK89 pKa = 9.75VLCVSDD95 pKa = 3.49VTRR98 pKa = 11.84GNGLTHH104 pKa = 6.89RR105 pKa = 11.84VGKK108 pKa = 9.66RR109 pKa = 11.84FCVKK113 pKa = 9.94SVYY116 pKa = 10.52VLGKK120 pKa = 9.41IWMDD124 pKa = 3.35EE125 pKa = 4.04NIKK128 pKa = 9.32TKK130 pKa = 10.59NHH132 pKa = 5.56TNTVIFFLVRR142 pKa = 11.84DD143 pKa = 3.76RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 10.16GSAMDD152 pKa = 4.13FGQVFNMYY160 pKa = 10.66DD161 pKa = 3.86NEE163 pKa = 4.36PSTATIKK170 pKa = 10.89NDD172 pKa = 3.0LRR174 pKa = 11.84DD175 pKa = 3.56RR176 pKa = 11.84YY177 pKa = 9.81QVLRR181 pKa = 11.84KK182 pKa = 9.77FSSTVTGGQYY192 pKa = 10.82ASKK195 pKa = 10.24EE196 pKa = 3.73QALVKK201 pKa = 10.62KK202 pKa = 10.37FMKK205 pKa = 9.91INNYY209 pKa = 8.31VVYY212 pKa = 10.35NHH214 pKa = 6.06QEE216 pKa = 3.36AAKK219 pKa = 10.14YY220 pKa = 10.5DD221 pKa = 3.75NHH223 pKa = 6.53TEE225 pKa = 4.0NALLFYY231 pKa = 8.02MACTHH236 pKa = 7.13ASNPVYY242 pKa = 9.86ATLKK246 pKa = 9.47IRR248 pKa = 11.84IYY250 pKa = 10.65FYY252 pKa = 11.33DD253 pKa = 3.43SVQNN257 pKa = 3.87

Molecular weight: 29.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1100

96

362

183.3

21.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.0 ± 0.712

2.545 ± 0.433

5.182 ± 0.282

4.727 ± 0.802

3.818 ± 0.283

4.273 ± 0.476

3.455 ± 0.434

5.182 ± 0.646

5.636 ± 0.856

7.545 ± 0.633

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.0 ± 0.508

6.364 ± 0.269

5.636 ± 0.523

5.636 ± 0.69

6.727 ± 1.095

8.0 ± 1.152

6.636 ± 1.062

5.727 ± 1.118

1.545 ± 0.343

4.364 ± 0.677
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski