Comamonas sp. BIGb0124

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Comamonas; unclassified Comamonas

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4360 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N1WZI9|A0A3N1WZI9_9BURK GTPase Era OS=Comamonas sp. BIGb0124 OX=2485130 GN=era PE=3 SV=1
MM1 pKa = 7.27SAVAEE6 pKa = 4.27QIVDD10 pKa = 3.84STVSSTDD17 pKa = 3.23EE18 pKa = 3.91MPLPFVFTDD27 pKa = 3.64SAAAKK32 pKa = 10.02VADD35 pKa = 5.45LIAEE39 pKa = 4.31EE40 pKa = 4.82GNPDD44 pKa = 2.99LKK46 pKa = 11.13LRR48 pKa = 11.84VFVQGGGCSGFQYY61 pKa = 10.94GFTFDD66 pKa = 4.69EE67 pKa = 4.93IVNEE71 pKa = 4.72DD72 pKa = 3.86DD73 pKa = 3.47TTLSKK78 pKa = 11.31NGVSLLIDD86 pKa = 3.54AMSYY90 pKa = 10.32QYY92 pKa = 11.5LVGAEE97 pKa = 3.79IDD99 pKa = 3.77YY100 pKa = 11.37KK101 pKa = 11.13EE102 pKa = 4.6DD103 pKa = 3.47LEE105 pKa = 4.54GAQFVIKK112 pKa = 10.53NPNATSTCGCGSSFSTT128 pKa = 4.55

Molecular weight: 13.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N1X7A4|A0A3N1X7A4_9BURK Putative aminoacrylate hydrolase RutD OS=Comamonas sp. BIGb0124 OX=2485130 GN=rutD PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4360

0

4360

1468464

29

2893

336.8

36.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.93 ± 0.052

0.832 ± 0.012

5.324 ± 0.029

4.933 ± 0.03

3.331 ± 0.024

8.492 ± 0.033

2.282 ± 0.021

4.306 ± 0.027

2.761 ± 0.036

10.934 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.308 ± 0.02

2.46 ± 0.024

5.48 ± 0.026

4.246 ± 0.024

7.082 ± 0.033

5.64 ± 0.03

5.242 ± 0.024

7.669 ± 0.032

1.468 ± 0.016

2.279 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski