Rhizophagus sp. RF1 medium virus

Taxonomy: Viruses; Riboviria; dsRNA viruses; unclassified dsRNA viruses

Average proteome isoelectric point is 7.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|E2RWR2|E2RWR2_9VIRU Putative coat protein OS=Rhizophagus sp. RF1 medium virus OX=758865 GN=CP PE=4 SV=1
MM1 pKa = 8.01RR2 pKa = 11.84LPPFKK7 pKa = 9.8PDD9 pKa = 2.95GVIYY13 pKa = 10.3EE14 pKa = 4.55SYY16 pKa = 11.04CSLFNLNDD24 pKa = 3.24IKK26 pKa = 11.38DD27 pKa = 4.01VFNAPANFQIMKK39 pKa = 9.72YY40 pKa = 10.02EE41 pKa = 4.58PIFVNSRR48 pKa = 11.84PRR50 pKa = 11.84YY51 pKa = 9.26LYY53 pKa = 10.01PHH55 pKa = 7.24DD56 pKa = 3.63WDD58 pKa = 3.98YY59 pKa = 11.94TDD61 pKa = 4.46GAGAGQSIEE70 pKa = 4.26VNGVRR75 pKa = 11.84CFFSDD80 pKa = 3.88IYY82 pKa = 9.8SCRR85 pKa = 11.84WNRR88 pKa = 11.84FNLNLYY94 pKa = 9.56SDD96 pKa = 4.16SVKK99 pKa = 10.64LVNPKK104 pKa = 10.11SQTVDD109 pKa = 3.1MTTLTPEE116 pKa = 4.29DD117 pKa = 4.84LMDD120 pKa = 4.47IDD122 pKa = 4.18QDD124 pKa = 3.38NTRR127 pKa = 11.84IYY129 pKa = 11.14VFGRR133 pKa = 11.84LDD135 pKa = 3.24LSHH138 pKa = 7.75PYY140 pKa = 9.98FSLDD144 pKa = 3.52FPSLEE149 pKa = 4.11SAATKK154 pKa = 9.98IRR156 pKa = 11.84PSLYY160 pKa = 8.85FARR163 pKa = 11.84DD164 pKa = 3.16GRR166 pKa = 11.84RR167 pKa = 11.84LVVPILASAIGKK179 pKa = 9.88NYY181 pKa = 8.64LTYY184 pKa = 10.62QNIRR188 pKa = 11.84NVIYY192 pKa = 10.6GCLDD196 pKa = 3.37DD197 pKa = 5.96RR198 pKa = 11.84DD199 pKa = 4.35NLPEE203 pKa = 3.92VYY205 pKa = 9.8KK206 pKa = 11.06FNVSWVCQQPFLPQDD221 pKa = 3.11KK222 pKa = 10.94DD223 pKa = 3.67FFSFNQEE230 pKa = 3.26VGRR233 pKa = 11.84WCFVATVNEE242 pKa = 4.05NTPLHH247 pKa = 6.12IKK249 pKa = 10.57YY250 pKa = 8.17LTKK253 pKa = 10.86NLLDD257 pKa = 3.58QMRR260 pKa = 11.84KK261 pKa = 8.96QMTNDD266 pKa = 2.65SSLRR270 pKa = 11.84HH271 pKa = 4.85YY272 pKa = 10.94QSTEE276 pKa = 3.57LSHH279 pKa = 7.02LNHH282 pKa = 6.2EE283 pKa = 4.74QFANWVVKK291 pKa = 10.15VKK293 pKa = 10.62NIKK296 pKa = 9.76GLHH299 pKa = 5.61EE300 pKa = 4.33KK301 pKa = 8.81TWDD304 pKa = 3.33KK305 pKa = 11.36YY306 pKa = 11.18RR307 pKa = 11.84LGSDD311 pKa = 3.57SVIDD315 pKa = 3.61QYY317 pKa = 11.82VINYY321 pKa = 7.39DD322 pKa = 3.75TAIKK326 pKa = 9.99EE327 pKa = 4.12YY328 pKa = 10.52QKK330 pKa = 11.63LLGSYY335 pKa = 8.89VNKK338 pKa = 10.1EE339 pKa = 4.11RR340 pKa = 11.84IDD342 pKa = 3.47IDD344 pKa = 4.11SNLVDD349 pKa = 4.07EE350 pKa = 5.02LSGLGSVNEE359 pKa = 3.89KK360 pKa = 10.67VKK362 pKa = 10.26WDD364 pKa = 3.46RR365 pKa = 11.84CGDD368 pKa = 3.46NRR370 pKa = 11.84DD371 pKa = 3.64RR372 pKa = 11.84LPFKK376 pKa = 10.9DD377 pKa = 4.95DD378 pKa = 4.18FYY380 pKa = 11.88NPDD383 pKa = 3.22EE384 pKa = 4.46SGRR387 pKa = 11.84RR388 pKa = 11.84YY389 pKa = 10.41SHH391 pKa = 6.6VFMNPSFKK399 pKa = 10.56IFGVRR404 pKa = 11.84LQRR407 pKa = 11.84LLSMLDD413 pKa = 3.3CVIDD417 pKa = 4.06VKK419 pKa = 10.77HH420 pKa = 6.88DD421 pKa = 3.62KK422 pKa = 10.72YY423 pKa = 11.18LQDD426 pKa = 3.57VFSMVYY432 pKa = 9.9CVAEE436 pKa = 3.79VSRR439 pKa = 11.84RR440 pKa = 11.84FKK442 pKa = 11.25LLGDD446 pKa = 3.71FMKK449 pKa = 10.34KK450 pKa = 10.28YY451 pKa = 10.72GSNLTKK457 pKa = 10.46DD458 pKa = 2.79WYY460 pKa = 11.13VYY462 pKa = 11.25VNLEE466 pKa = 3.75TAIGYY471 pKa = 7.59KK472 pKa = 9.1TALPTDD478 pKa = 4.09EE479 pKa = 4.31FVHH482 pKa = 6.46MGGEE486 pKa = 4.2WIVGDD491 pKa = 4.23IEE493 pKa = 5.51HH494 pKa = 7.29IAPVPNGQLKK504 pKa = 9.45FYY506 pKa = 9.04EE507 pKa = 4.13WLVEE511 pKa = 4.55GIEE514 pKa = 4.09STLRR518 pKa = 11.84LKK520 pKa = 10.86GKK522 pKa = 10.14SKK524 pKa = 10.41IMYY527 pKa = 7.1EE528 pKa = 4.03KK529 pKa = 10.23PIEE532 pKa = 4.17FDD534 pKa = 3.03EE535 pKa = 5.18HH536 pKa = 6.13GNPMEE541 pKa = 4.3TLDD544 pKa = 3.54QFSYY548 pKa = 11.12DD549 pKa = 3.18VSKK552 pKa = 10.72QLSYY556 pKa = 11.0RR557 pKa = 11.84DD558 pKa = 3.67YY559 pKa = 11.45VYY561 pKa = 10.61GCTWSRR567 pKa = 11.84RR568 pKa = 11.84GSSSIKK574 pKa = 8.28TKK576 pKa = 10.65LRR578 pKa = 11.84AEE580 pKa = 4.11INGKK584 pKa = 8.31KK585 pKa = 10.22VKK587 pKa = 10.61LSEE590 pKa = 4.38TKK592 pKa = 9.17NTLAIYY598 pKa = 10.72GDD600 pKa = 3.85LEE602 pKa = 5.03KK603 pKa = 10.5ILKK606 pKa = 10.42DD607 pKa = 3.34SLDD610 pKa = 3.54PDD612 pKa = 3.71RR613 pKa = 11.84PQWGKK618 pKa = 10.81AIRR621 pKa = 11.84KK622 pKa = 9.16RR623 pKa = 11.84EE624 pKa = 3.87RR625 pKa = 11.84GKK627 pKa = 10.1IRR629 pKa = 11.84AVLGMDD635 pKa = 3.49VEE637 pKa = 5.31TYY639 pKa = 11.24LPMSYY644 pKa = 9.45IDD646 pKa = 6.28DD647 pKa = 4.91IIQPYY652 pKa = 10.11IIGNEE657 pKa = 3.96LSNLWEE663 pKa = 4.41TNFQRR668 pKa = 11.84HH669 pKa = 5.56AKK671 pKa = 10.3DD672 pKa = 2.96VDD674 pKa = 3.69RR675 pKa = 11.84LILSIEE681 pKa = 3.92KK682 pKa = 9.31TKK684 pKa = 10.52WFIPLDD690 pKa = 3.45QSGFDD695 pKa = 3.45HH696 pKa = 7.15QINNNMLLLTLVIIKK711 pKa = 9.84SWLLSRR717 pKa = 11.84VKK719 pKa = 10.96YY720 pKa = 10.51KK721 pKa = 11.11GDD723 pKa = 3.33VEE725 pKa = 4.25RR726 pKa = 11.84CIEE729 pKa = 4.2IIIKK733 pKa = 10.32RR734 pKa = 11.84LALTNSEE741 pKa = 4.41VNVDD745 pKa = 3.85DD746 pKa = 5.43KK747 pKa = 11.75INIPVEE753 pKa = 4.0RR754 pKa = 11.84GILSGWKK761 pKa = 7.04WTAFMDD767 pKa = 3.57TLINIGEE774 pKa = 4.36VNVALRR780 pKa = 11.84TCEE783 pKa = 3.91YY784 pKa = 10.62YY785 pKa = 11.23GLVCNYY791 pKa = 10.41KK792 pKa = 10.36LDD794 pKa = 4.0AQGDD798 pKa = 3.95DD799 pKa = 4.38DD800 pKa = 6.96DD801 pKa = 6.99IIIDD805 pKa = 4.15DD806 pKa = 4.76LLTGLVMCEE815 pKa = 3.49AYY817 pKa = 10.08RR818 pKa = 11.84YY819 pKa = 9.91CNLKK823 pKa = 10.23INPAKK828 pKa = 10.46FFIKK832 pKa = 10.74NNIDD836 pKa = 3.08EE837 pKa = 4.48YY838 pKa = 11.03LRR840 pKa = 11.84RR841 pKa = 11.84VYY843 pKa = 11.11VDD845 pKa = 4.0GVSLGYY851 pKa = 8.48PARR854 pKa = 11.84MFLTFFEE861 pKa = 5.1SNPIRR866 pKa = 11.84NVPLSEE872 pKa = 3.9MSEE875 pKa = 3.9LRR877 pKa = 11.84SVIKK881 pKa = 10.7NWMDD885 pKa = 4.77LIRR888 pKa = 11.84RR889 pKa = 11.84RR890 pKa = 11.84SLNNEE895 pKa = 3.47RR896 pKa = 11.84WVKK899 pKa = 10.02LVKK902 pKa = 10.67LMEE905 pKa = 4.75RR906 pKa = 11.84DD907 pKa = 2.89ILIRR911 pKa = 11.84VNKK914 pKa = 8.77NRR916 pKa = 11.84KK917 pKa = 8.77ISSLSLEE924 pKa = 4.57TILL927 pKa = 5.58

Molecular weight:
108.29 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|E2RWR2|E2RWR2_9VIRU Putative coat protein OS=Rhizophagus sp. RF1 medium virus OX=758865 GN=CP PE=4 SV=1
MM1 pKa = 7.71SLRR4 pKa = 11.84LRR6 pKa = 11.84INTKK10 pKa = 9.77GLAVVIPSGMGKK22 pKa = 7.1TTLSNKK28 pKa = 9.55FPSLFLDD35 pKa = 3.73IDD37 pKa = 4.63DD38 pKa = 5.05IVWKK42 pKa = 9.71KK43 pKa = 10.32HH44 pKa = 5.94KK45 pKa = 10.24ILIGIINKK53 pKa = 9.32AFAWKK58 pKa = 10.18FGSDD62 pKa = 3.14LTKK65 pKa = 10.92NILRR69 pKa = 11.84RR70 pKa = 11.84KK71 pKa = 8.02WLWNDD76 pKa = 3.22TLSRR80 pKa = 11.84KK81 pKa = 9.35VLLCHH86 pKa = 7.01SPDD89 pKa = 3.54QLPSGVRR96 pKa = 11.84VLCILNSNSLNVNKK110 pKa = 10.09IKK112 pKa = 10.74KK113 pKa = 8.27EE114 pKa = 3.8RR115 pKa = 11.84KK116 pKa = 8.42ADD118 pKa = 3.5NVRR121 pKa = 11.84ISLQNRR127 pKa = 11.84HH128 pKa = 5.88SLWEE132 pKa = 3.89NYY134 pKa = 9.46RR135 pKa = 11.84DD136 pKa = 3.49EE137 pKa = 4.52EE138 pKa = 4.72NYY140 pKa = 10.53FEE142 pKa = 4.26VQSNKK147 pKa = 9.65VMLDD151 pKa = 3.33TIINCLIKK159 pKa = 10.92YY160 pKa = 10.39VDD162 pKa = 4.21FEE164 pKa = 4.57KK165 pKa = 11.12PNDD168 pKa = 3.61NTKK171 pKa = 10.9LLVPSKK177 pKa = 10.79DD178 pKa = 3.73YY179 pKa = 10.59IGKK182 pKa = 9.1FVRR185 pKa = 11.84KK186 pKa = 8.95EE187 pKa = 3.18RR188 pKa = 11.84YY189 pKa = 8.8LRR191 pKa = 11.84YY192 pKa = 9.19KK193 pKa = 9.98VKK195 pKa = 10.81YY196 pKa = 9.55DD197 pKa = 3.04ISEE200 pKa = 4.77PIVMSVRR207 pKa = 11.84EE208 pKa = 3.84YY209 pKa = 11.43MMIKK213 pKa = 10.25DD214 pKa = 4.01YY215 pKa = 10.89WRR217 pKa = 11.84DD218 pKa = 3.38LYY220 pKa = 10.16VHH222 pKa = 6.92RR223 pKa = 11.84KK224 pKa = 4.96WFKK227 pKa = 9.26IEE229 pKa = 4.54SYY231 pKa = 11.25GKK233 pKa = 9.73DD234 pKa = 3.29EE235 pKa = 4.2KK236 pKa = 11.14HH237 pKa = 6.58SLTDD241 pKa = 3.35EE242 pKa = 4.35SVNDD246 pKa = 3.72QIVTKK251 pKa = 10.29ILPLIDD257 pKa = 4.38KK258 pKa = 7.63EE259 pKa = 4.62TLFVLKK265 pKa = 10.1EE266 pKa = 3.97YY267 pKa = 10.39RR268 pKa = 11.84WDD270 pKa = 3.69RR271 pKa = 11.84MITNLMGIKK280 pKa = 10.24NSEE283 pKa = 4.17LVTNWMYY290 pKa = 10.87TPAYY294 pKa = 9.18LGGVGIQLDD303 pKa = 4.3GLRR306 pKa = 11.84KK307 pKa = 8.16PMSYY311 pKa = 8.39ITSSEE316 pKa = 4.27SIITPFVKK324 pKa = 10.08YY325 pKa = 9.55PKK327 pKa = 9.83SEE329 pKa = 3.97RR330 pKa = 11.84VEE332 pKa = 4.26NLTDD336 pKa = 3.98MILKK340 pKa = 9.97RR341 pKa = 11.84SKK343 pKa = 10.69FSVVDD348 pKa = 3.96NIQVYY353 pKa = 10.01EE354 pKa = 4.54GSQSTNKK361 pKa = 9.44IFKK364 pKa = 10.27SVGILRR370 pKa = 11.84FHH372 pKa = 7.07KK373 pKa = 10.95VIDD376 pKa = 4.27NINFRR381 pKa = 11.84KK382 pKa = 9.14FEE384 pKa = 4.01WMISGVDD391 pKa = 3.95FCKK394 pKa = 10.95SMIRR398 pKa = 11.84GVEE401 pKa = 4.0LPIKK405 pKa = 9.61MIRR408 pKa = 11.84PIFNSNIRR416 pKa = 11.84INQHH420 pKa = 5.77AQTIWINGLMRR431 pKa = 11.84AKK433 pKa = 10.61DD434 pKa = 3.6EE435 pKa = 4.87EE436 pKa = 4.32ITNEE440 pKa = 3.72ILSEE444 pKa = 4.07LFVNVSEE451 pKa = 5.62IIDD454 pKa = 3.46KK455 pKa = 10.61RR456 pKa = 11.84RR457 pKa = 11.84NFSKK461 pKa = 10.55IFWRR465 pKa = 11.84DD466 pKa = 3.23WLCGRR471 pKa = 11.84LEE473 pKa = 4.05YY474 pKa = 10.45QKK476 pKa = 10.33PINMWINDD484 pKa = 4.4NIVSTYY490 pKa = 10.33FIINLNRR497 pKa = 11.84LLSLNCNVDD506 pKa = 3.33YY507 pKa = 11.52GMLKK511 pKa = 10.51SMCKK515 pKa = 9.71IIEE518 pKa = 4.13KK519 pKa = 9.89TEE521 pKa = 3.87LNNIIRR527 pKa = 11.84YY528 pKa = 8.74LRR530 pKa = 11.84EE531 pKa = 3.63RR532 pKa = 11.84KK533 pKa = 7.96ITLMM537 pKa = 4.36

Molecular weight:
63.59 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1464

537

927

732.0

85.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.391 ± 0.589

1.503 ± 0.108

7.104 ± 0.823

5.464 ± 0.066

4.167 ± 0.139

4.508 ± 0.425

1.434 ± 0.071

8.675 ± 1.254

8.06 ± 0.88

9.973 ± 0.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.801 ± 0.4

6.967 ± 0.463

3.415 ± 0.337

2.459 ± 0.324

6.352 ± 0.09

6.899 ± 0.197

3.962 ± 0.073

6.831 ± 0.17

2.254 ± 0.191

4.781 ± 0.472

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski