Rickettsiales endosymbiont of Peranema trichophorum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; unclassified Rickettsiales

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q7DHE3|A0A4Q7DHE3_9RICK 50S ribosomal protein L3 OS=Rickettsiales endosymbiont of Peranema trichophorum OX=2486577 GN=rplC PE=3 SV=1
MM1 pKa = 7.41EE2 pKa = 5.78ARR4 pKa = 11.84LEE6 pKa = 4.01MCMKK10 pKa = 9.29GTEE13 pKa = 3.87RR14 pKa = 11.84KK15 pKa = 9.47LWVDD19 pKa = 3.05ADD21 pKa = 3.69MDD23 pKa = 4.02NDD25 pKa = 3.54IDD27 pKa = 3.95VLVYY31 pKa = 8.53TRR33 pKa = 11.84RR34 pKa = 11.84DD35 pKa = 3.45GSSSICYY42 pKa = 9.41CEE44 pKa = 5.39SMDD47 pKa = 5.84DD48 pKa = 4.0EE49 pKa = 5.31LSSSAYY55 pKa = 9.55GHH57 pKa = 6.05EE58 pKa = 4.17NCVTVDD64 pKa = 3.45EE65 pKa = 4.82LLL67 pKa = 4.36

Molecular weight: 7.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7DM92|A0A4Q7DM92_9RICK ParA family protein OS=Rickettsiales endosymbiont of Peranema trichophorum OX=2486577 GN=EDM53_01630 PE=4 SV=1
MM1 pKa = 6.85ATKK4 pKa = 9.46RR5 pKa = 11.84TYY7 pKa = 10.2QPSVLVRR14 pKa = 11.84KK15 pKa = 9.31RR16 pKa = 11.84RR17 pKa = 11.84HH18 pKa = 4.46GFRR21 pKa = 11.84ARR23 pKa = 11.84MSTVGGRR30 pKa = 11.84KK31 pKa = 9.23VISNRR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 10.18GRR41 pKa = 11.84HH42 pKa = 5.33ALSAA46 pKa = 3.9

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1129

0

1129

390504

25

3162

345.9

38.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.685 ± 0.065

1.286 ± 0.026

5.348 ± 0.053

6.499 ± 0.093

3.92 ± 0.045

6.725 ± 0.092

2.295 ± 0.039

7.683 ± 0.074

6.696 ± 0.065

10.109 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.543 ± 0.038

4.418 ± 0.044

3.198 ± 0.056

3.833 ± 0.081

4.641 ± 0.056

7.195 ± 0.051

5.195 ± 0.062

7.271 ± 0.07

0.874 ± 0.027

3.587 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski